CBMA

Study of Lactoferrin's anticancer mechanism of action.

Phosphoregulation of Bax-dependent cell death.

Pereira CS, Guedes JP, Gonçalves M, Loureiro L, Castro L, Gerós H, Rodrigues LR, Côrte-Real M (2016) Lactoferrin selectively triggers apoptosis in highly metastatic breast cancer cells through inhibition of plasmalemmal V-H+-ATPase. Oncotarget. DOI 10.18632/oncotarget.11394

Guedes JP, Pereira CS, Rodrigues LR, Côrte-Real M (2018) Bovine Milk Lactoferrin Selectively Kills Highly Metastatic Prostate Cancer PC-3 and Osteosarcoma MG-63 Cells In Vitro. Frontiers in Oncology. doi.org/10.3389/fonc.2018.00200

Acosta-Zaldívar M, Andrés MT, Rego A, Pereira CS, Fierro JF, Côrte-Real M (2015) Human lactoferrin triggers a mitochondrial- and caspase-dependent regulated cell death in Saccharomyces cerevisiae. Apoptosis. DOI 10.1007/s10495-015-1199-9.

Study of the anticancer activity of lactoferrin

 "Phosphoregulation of Bax-dependent cell death", FCT-ANR/BEX-BCM/0175/2012 

Guedes JP, Pereira CS, Rodrigues LR, Côrte-Real M (2018) Bovine Milk Lactoferrin Selectively Kills Highly Metastatic Prostate Cancer PC-3 and Osteosarcoma MG-63 Cells In Vitro. Frontiers in Oncology. doi.org/10.3389/fonc.2018.00200

Pereira CS, Guedes JP, Gonçalves M, Loureiro L, Castro L, Gerós H, Rodrigues LR, Côrte-Real M (2016) Lactoferrin selectively triggers apoptosis in highly metastatic breast cancer cells through inhibition of plasmalemmal V-H+-ATPase. Oncotarget. DOI 10.18632/oncotarget.11394

Acosta-Zaldívar M, Andrés MT, Rego A, Pereira CS, Fierro JF, Côrte-Real M (2015) Human lactoferrin triggers a mitochondrial- and caspase-dependent regulated cell death in Saccharomyces cerevisiae. Apoptosis. DOI 10.1007/s10495-015-1199-9.

Rodrigues J*, Pereira CS*, Fontes N, Gerós H, Côrte-Real M (2018) Flow Cytometry and Fluorescence Microscopy as Tools for Structural and Functional Analysis of Vacuoles Isolated from Yeast and Plant Cells. In Cláudia Pereira (Eds), Plant Vacuolar Trafficking, vol. 1789, chapter 8. ISBN: 978-1-4939-7855-7. *Both authors contributed equally

Oral Communications:

5. Pereira CS, Guedes JP, Chaves SR, Gerós H, Rodrigues LR, Côrte-Real M. (2018) "Deciphering the mechanisms underlying bovine milk lactoferrin anticancer activity using yeast and cancer cell lines as complementary models”, XXI Jornadas de Biologia de Leveduras “Professor van Uden, 8-9/6, Braga, Portugal

 4. Pereira CS (2018) "How can a milk protein selectively kill cancer cells?". DBio seminairs, 7/2, Braga, Portugal (invited speaker)

3. Pereira CS, Guedes JP, Chaves SR, Gerós H, Rodrigues LR, Côrte-Real M (2017) "Understanding the interplay between the pro-apoptotic protein lactoferrin and biomembranes: from yeast to human cells". 12^th International meeting on yeast apoptosis (IMYA), 14/6 –18/6, Bari, Italy (FEMS grant)

2. Pereira CS, Gonçalves M, Loureiro L, Castro L, Gerós H, Rodrigues LR, Côrte-Real M. (2015) "Lactoferrin selectively triggers apoptosis in highly metastatic breast cancer cells through inhibition of plasmalemal V-H⁺-ATPase". III Symposium of the PhD Programme on Molecular and Environmental Biology, 26/11, Braga, Portugal. (Best presentation award)

1. Castro L, Alves S, Pereira CS, Chaves SR, Sousa MJ, Manon S, Côrte-Real M. (2015) "Yeast as a tool to identify novel kinases involved in regulation of Bax". 11^th International meeting on yeast apoptosis (IMYA), 29/9 – 3/10, Porto, Portugal.

Poster Communications:

20. Pereira CS, Guedes JP, Chaves SR, Gerós H, Rodrigues LR, Côrte-Real M. (2018) "Lactoferrin perturbs intracellular trafficking in yeast and highly metastatic cancer cells". 2nd Small Meeting on Endocytic Trafficking and Signalling (SMETS2), 10-12/7, Coimbra, Portugal.

19. Baptista V, Pereira CS, Guedes JP, Sousa MJ, Chaves SC and Côrte-Real M. (2018) " Yeast as a cell model to study the human pro-apoptotic protein Bax ", Jornadas de Biologia de Leveduras “XXI Professor van Uden, 8-9/6, Braga, Portugal

 18. Terra-Matos J, Pereira CS, Sousa MJ, Chaves SC, Gerós H and Côrte-Real M. (2018) " Role of vacuolar membrane proteins in acetic acid-induced cell death ", Jornadas de Biologia de Leveduras “XXI Professor van Uden, 8-9/6, Braga, Portugal

 17. Pereira CS, Guedes JP, Chaves SR, Gerós H, Rodrigues LR, Côrte-Real M. (2018) "Exploring a novel effec of lactoferrin on the plasma membrane towards the elucidation of the mechanisms of action: from yeast to human cells", Jornadas de Bioquímica – Da investigação à indústria, 10-11/4, Braga, Portugal (invitation)

16. Baptista V, Pereira CS, Guedes JP, Sousa MJ, Chaves SC and Côrte-Real M. (2018) "Yeast as a cell model to study the human pro-apoptotic protein Bax ", Jornadas de Bioquímica – Da investigação à indústria, 10-11/4, Braga, Portugal

15. Pereira CS, Guedes JP, Chaves SR, Gerós H, Rodrigues LR, Côrte-Real M. (2018) "Lactoferrin perturbs intracellular trafficking in yeast and highly metastatic cancer cells". 1^st Meeting on Vesicular Biology, 16-17/2, Coimbra, Portugal.

14. Pereira CS, Guedes JP, Chaves SR, Gerós H, Rodrigues LR, Côrte-Real M. (2017) "Exploring a novel effec of lactoferrin on the plasma membrane towards the elucidation of the mechanisms of action: from yeast to human cells". Microbiotec 17, 7-9/12, Porto, Portugal. (Best Poster Communication award)

13. Pereira H*, Alves S*, Pereira CS*, Oliveira CFS, Moreira T, Preto A, Chaves SR^#, Côrte-Real M^# (2017) "Mitochondrial  ROS accumulation sensitizes colorectal cancer cells to acetate and Cathepsin D inhibitors combination therapy". International symposium MitoPorto - Advances in mitochondrial research. 26/5, Porto, Portugal.

12. Pereira CS, Guedes JP, Gonçalves M, Loureiro L, Castro L, Gerós H, Rodrigues LR, Côrte-Real M. (2016) "Lactoferrin selectively triggers apoptosis in highly metastatic breast cancer cells through inhibition of plasmalemal V-H+-ATPase". XIX National Congress of Biochemistry, 8-10/12, Guimarães, Portugal.

11. Guedes JP, Pereira CS, Rodrigues LR, Côrte-Real M. (2016) "Insights on the Molecular Mechanisms Underlying the Anticancer Activity of Lactoferrin in Highly Metastatic Cancer Cell Lines". XIX National Congress of Biochemistry, 8-10/12, Guimarães, Portugal.

10. Pereira CS, Guedes JP, Gonçalves M, Loureiro L, Castro L, Gerós H, Rodrigues LR, Côrte-Real M. (2016) "Lactoferrin selectively triggers apoptosis in highly metastatic breast cancer cells through inhibition of plasmalemal V-H+-ATPase". 24th  congress of the European Cell Death Organization - "Cell Death in Health and Disease", 28-30/09, Barcelona, Spain.

9. Castro L, Alves S, Pereira CS, Chaves SR, Sousa MJ, Manon S, Côrte-Real M. (2015) "Yeast as a tool to identify novel kinases involved in regulation of Bax". Microbiotec, 10-12/12, Évora, Portugal. 

8. Castro L, Alves S, Pereira CS, Chaves SR, Sousa MJ, Manon S, Côrte-Real M. (2015) "Yeast as a system model to characterize Bax regulation by individual kinases". III Symposium of the PhD Programme on Molecular and Environmental Biology, 26/11, Braga, Portugal.

7. Pereira CS, Gonçalves M, Loureiro L, Castro L, Gerós H, Rodrigues LR, Côrte-Real M. (2015) "Lactoferrin selectively triggers apoptosis in highly metastatic breast cancer cells through inhibition of plasmalemal V-H+-ATPase". III Symposium of the PhD Programme on Molecular and Environmental Biology, 26/11, Braga, Portugal.

6. Castro L, Alves S, Pereira CS, Chaves SR, Sousa MJ, Manon S, Côrte-Real M. (2015) "Yeast as a tool to identify novel kinases involved in regulation of Bax". 11^th International meeting on yeast apoptosis (IMYA), 29/9 – 3/10, Porto, Portugal. 

5. Pereira CS, Gonçalves M, Loureiro L, Castro L, Gerós H, Rodrigues LR, Côrte-Real M. (2014) “Search for mechanisms underlying the anti-tumoral activity of lactoferrin”. II Symposium of the PhD Programme on Molecular and Environmental Biology, 27/11, Braga, Portugal.

4. Gonçalves M, Loureiro L, Pereira CS, Gerós H, Côrte-Real M. (2014) “An in vitro approach towards the elucidation of the mechanisms of action of lactoferrin”. II Symposium of the PhD Programme on Molecular and Environmental Biology, 27/11, Braga, Portugal.

3. Loureiro L, Gonçalves M, Pereira CS, Côrte-Real M, Gerós H. (2014) “Functional characterization of mitochondrial suspensions by flow cytometry towards the screening of mitochondrial drug targets”. II Symposium of the PhD Programme on Molecular and Environmental Biology, 27/11, Braga, Portugal.

2. Acosta-Zaldivar M, Andrés MT, Rego A, Pereira CS, Côrte-Real M, Fierro JF (2014) “Human lactoferrin triggers a caspase- and mitochondrial-dependent programmed cell death in Saccharomyces cerevisiae”. 10^th International meeting on yeast apoptosis (IMYA), 14-18/5, Gothenburg, Sweden.

1. Acosta-Zaldivar M, Andrés MT, Rego A, Pereira CS, Côrte-Real M, Fierro JF (2014) “Mitochondria involvement in human lactoferrin-induced programmed cell death in Saccharomyces cerevisiae”. XX Jornadas de Biologia de Leveduras Professor Nicolau van Uden, 11-12/7, Lisbon, Portugal.