CBMA

     At the present time I am interested in the identification and characterization of novel carboxylate transporters, with a potential biotechnological application. 

       Several carboxylic acids are among the top value added chemicals produced from biomass, as these building-blocks can be subsequently converted to a number of high-value bio-based chemicals or materials. Different strategies have been developed to improve the cell factory production of these acids; however the export of these molecules is still a major bottleneck. In our group the study of carboxylic acid transporters has been one of the major focuses of research for several years now. The characterization of these transporters is done both at the functional and molecular levels. Transporter proteins are characterized by heterologous expression in a Saccharomyces cerevisiae strain with no carrier-mediated carboxylate transport at the plasma membrane. In addition, structure-function studies are conducted to unravel the mechanisms of substrate recognition and specificity of these proteins.

ORCID ID: 0000-0001-8431-1567,

Scopus Author ID: 6506016645

Publons publons.com/a/1178742/

Researchgate Isabel_Soares-Silva

David Ribas, Joana Sá-Pessoa, Isabel Soares-Silva, Sandra Paiva, Yvonne Nygård, Laura Ruohonen, Merja Penttilä, Margarida Casal; Yeast as a tool to express sugar acid transporters with biotechnological interest. FEMS Yeast Res 2017 fox005. doi: 10.1093/femsyr/fox005.

Soares-Silva I, Ribas D, Foskolou IP, Barata B, Bessa D, Paiva S, Queirós O, Casal M. 2015 The Debaryomyces hansenii carboxylate transporters Jen1 homologues are functional in Saccharomyces cerevisiae. FEMS Yeast Res.  Dec;15(8). 

Soares-Silva I, Sá-Pessoa J, Myrianthopoulos V, Mikros E, Casal M, Diallinas G. 2011. A substrate translocation trajectory in a cytoplasm-facing topological model of the monocarboxylate/H(+) symporter Jen1p, Mol Microbiol 81(3):805-17

Casal, M., Paiva, S., Queirós, O., Soares-Silva, I. 2008. Transport of carboxylic acids in yeast, FEMS Microbiol Rev 32(6):974-94. 

Soares-Silva, I., Paiva,S., Diallinas, G. and Casal, M., 2007. The conserved sequence NXX[S/T]HX[S/T]QDXXXT of the lactate/pyruvate:H⁺ symporter subfamily defines the function of the substrate translocation pathway. Molecular Membrane Biology, 24, 464-74.

Soares-Silva, I., Paiva, S., Kötter, P., Entian, K.-D., Casal M. 2004. The disruption of JEN1 from Candida albicans impairs the transport of lactate. Molecular Membrane Biology, 21, 403-411. 

Soares-Silva, I., Schuller, D., Andrade, R. P., Baltazar, F., Cássio F., Casal M. 2003. Functional expression of the lactate permease Jen1p of Saccharomyces cerevisiae in Pichia pastoris. Biochemical Journal, 376, 781-787. 

Principal Investigator 

2014-2016

Establishment of an in vitro model system for the study of biofilm formation in hemodialysis tunneled catheters Financed by ESCMID Research Grants 2014

Team member

2016-2018

Trans-acids. Structural functional studies of carboxylic acid transporters: Novel tools in production optimization using industrial microbes (PTDC/BIA-MIC/5184/2014)

2016-2019

EcoAgriFood: Innovative green products and processes to promote Agri-Food BioEconomy  (operação NORTE-01-0145-FEDER-000009)

Team member

JENesis -Molecular and genetic analysis of monocarboxylic acid transporters in yeasts. Financed by Portuguese Foundation for Science (FCT) POCTI/1999/BME/36625.

2005-2008

PermYeast – Molecular biology of the monocarboxylic acids permeases in yeast: from functional to structural analysis. Financed by Portuguese Foundation for Science (FCT) POCTI/ BMC/57812/2004.

2008-2011

Increased cardiovascular risk in chronic renal insufficiency. Role of the novel monoamine-oxidase renalase in the sympathetic and renal dopaminergic activities Financed by Portuguese Foundation for Science (FCT) PIC/IC/83029/2007

2011-2013

Mechanisms of natriuretic peptide system dysfunction in nephrotic syndrome: relationship with reduced renal dopaminergic system activity and vitamin D3 deficiency Financed by Portuguese Nephrology Society 2011

2011-2013

Fibroblast Growth Factor 23 (FGF-23) in patients on peritoneal dialysis: relationship with peritoneal transport and cardiovascular outcome Financed by Baxter Clinical Evidence Council in 2011

2014-2015

Consolidação das Competências e Recursos de Investigação em Biotecnologia Celular e Molecular no CEB/IBB. Financed by Portuguese Foundation for Science (FCT) (FCT RECI/BBB-EBI/0179/2012)

1. Soares-Silva, I., Schuller, D., Andrade, R. P., Baltazar, F., Cássio F., Casal M. 2003. Functional expression of the lactate permease Jen1p of Saccharomyces cerevisiae in Pichia pastoris. Biochemical Journal, 376, 781-787. IF 4.101

2. Soares-Silva, I., Paiva, S., Kötter, P., Entian, K.-D., Casal M. 2004. The disruption of JEN1 from Candida albicans impairs the transport of lactate. Molecular Membrane Biology, 21, 403-411. IF 5.667

3. Soares-Silva, I., Paiva, S., Diallinas, G. and Casal, M., 2007. The conserved sequence NXX[S/T]HX[S/T]QDXXXT of the lactate/pyruvate:H⁺ symporter subfamily defines the function of the substrate translocation pathway. Molecular Membrane Biology, 24, 464-74. IF 3.870

4. Oliveira, J., Soares-Silva, I., Fokkema, I., Gonçalves, A., Cabral, A., Diogo, L., Galán, L., Guimarães, A., Fineza, I., den Dunnen, J. T., Santos, R. 2008. New synonymous substitution in POMGNT1 promotes exon skipping in a CMD patient, J Human Gen 53(6):565-72 IF 2.431

5. Oliveira J., Santos R., Soares-Silva I., Jorge P., Vieira E., Oliveira M., Moreira A., Coelho T., Ferreira J., Fonseca M., Barbosa C., Prats J., Aríztegui M., Martins M., Moreno T., Heinimann K., Barbot C., Pascual-Pascual S., Cabral A., Fineza I., Santos M., Bronze-da-Rocha E. 2008. LAMA2 gene analysis in a cohort of 26 congenital muscular dystrophy patients, Clin Genet 74(6):502-12 IF 3.206

6. Casal, M., Paiva, S., Queirós, O., Soares-Silva, I. 2008. Transport of carboxylic acids in yeast, FEMS Microbiol Rev 32(6):974-94. IF 7.963

7. Soares-Silva I, Sá-Pessoa J, Myrianthopoulos V, Mikros E, Casal M, Diallinas G. 2011. A substrate translocation trajectory in a cytoplasm-facing topological model of the monocarboxylate/H(+) symporter Jen1p, Mol Microbiol 81(3):805-17 IF 5.010

8. Simões-Silva, L., Moreira-Rodrigues, M., Quelhas-Santos, J., Fernandes-Cerqueira, C., Pestana, M., Soares-Silva, I., Sampaio-Maia, B. 2013 Intestinal and renal guanylin peptides system in hypertensive obese mice Exp Biol Med 238(1):90-7 IF 2012 2.226

9. Quelhas-Santos J; Sampaio-Maia B; Fernandes-Cerqueira C; Serrão P; Simões-Silva L; Moreira-Rodrigues M; Soares-Silva I; Pestana M, 2013 Sodium dependent decrease activity of renalase in the rat remnant kidney J Hypertens. 31(3):543-52. IF 2012 4.222

10. Quelhas-Santos J, Soares-Silva I, Fernandes-Cerqueira C, Simões-Silva L, Ferreira I, Carvalho C , Coentrão L,  Vaz R, Sampaio-Maia B; Pestana M, 2014 Plasma and Urine Renalase Levels and Activity During the Recovery of Renal Function in Kidney Transplant Recipients  Exp Biol Med ;239(4):502-811. IF 2012 2.803

11. Quelhas-Santos J, Serrão P, Soares-Silva I, Fernandes-Cerqueira C, Simões-Silva L, Pinho MJ, Remião F, Sampaio-Maia B, Desir G, Pestana M. 2015 Renalase regulates peripheral and central dopaminergic activities  American Journal of Physiology - Renal Physiology; 308 (2), F84-F91  IF 2014 3.248

12. Quelhas-Santos J, Sampaio-Maia B, Remião F, Serrão P, Soares-Silva I, Desir G, Pestana M 2015 “Assessment of renalase activity on catecholamines degradation” The Open Hypertension Journal; 7: 14-18. 

13. Soares-Silva I, Ribas D, Foskolou IP, Barata B, Bessa D, Paiva S, Queirós O, Casal M. 2015 The Debaryomyces hansenii carboxylate transporters Jen1 homologues are functional in Saccharomyces cerevisiae. FEMS Yeast Res. Dec;15(8).

14. Sarmento-Dias M , Santos-Araújo C, Poínhos R, Oliveira B, Sousa M, Simões Silva L, Soares Silva I, Correia F and Pestana M, 2015, Associations between Peritoneal Daily Phosphate Removal and Regulators of Phosphate Metabolism Including Fibroblast Growth Factor 23 in Peritoneal Dialysis Patients. J Kidney 1: 109. doi:10.4172/2427-1220.1000109 

15. Sarmento-Dias M., C. Santos-Araújo, R. Poínhos, B. Oliveira, M. Sousa, L. Simões-Silva, I. Soares-Silva, F. Correia, M. Pestana, 2016 Fibroblast Growth Factor 23 is associated with Left Ventricular Hypertrophy, Not with Uremic Vasculopathy in Peritoneal Dialysis Patients" Clin Nephrol. Mar;85(3):135-41. doi: 10.5414/CN108716.

16. Simões-Silva L, Correia I, Barbosa J, Sousa MJ, Santos-Araújo C, Pestana M, Soares-Silva, I; Sampaio-Maia, B, 2016 Asymptomatic effluent protozoa colonization in peritoneal dialysis patients Perit Dial Int September-October 2016 vol. 36 no. 5 566-569.

17. Correia I, Simões-Silva L, Santos-Araujo C, Sousa MJ, Pestana M, Soares-Silva I, Sampaio-Maia B, Barbosa J 2016 Low prevalence of oral protozoa colonization in Portuguese healthy and end-stage renal disease patients undergoing peritoneal dialysis Boletín de Malariología y Salud Ambiental, Vol. LVI (1): 11-18.

18. Sarmento-Dias M., C. Santos-Araújo, R. Poínhos, B. Oliveira, M. Sousa, L. Simões-Silva, I. Soares-Silva, F. Correia, M. Pestana, 2017 Phase angle predicts arterial stiffness and vascular calcification in peritoneal dialysis patients Perit Dial Int. 2017 pii: pdi.2015.00276. doi: 10.3747/pdi.2015.00276.

19. David Ribas, Joana Sá-Pessoa, Isabel Soares-Silva, Sandra Paiva, Yvonne Nygård, Laura Ruohonen, Merja Penttilä, Margarida Casal; Yeast as a tool to express sugar acid transporters with biotechnological interest. FEMS Yeast Res 2017 fox005. doi: 10.1093/femsyr/fox005.

20. Liliana Simões-Silva, Sara Silva, Carla Santos-Araujo, Joana Sousa, Manuel Pestana, Ricardo Araujo, Isabel Soares-Silva, and Benedita Sampaio-Maia 2017 “Oral Yeast Colonization and Fungal Infections in Peritoneal Dialysis Patients: A Pilot Study,” Canadian Journal of Infectious Diseases and Medical Microbiology, vol. 2017, Article ID 4846363, 7 pages. doi:10.1155/2017/4846363

21. Cátia Fernandes Cerqueira, Janete Santos, Benedita Sampaio-Maia, Liliana Simões-Silva, Isabel J. Soares-Silva, Roberto Roncon-Albuquerque Jr and Manuel Pestana, Calcitriol Prevents Cardiovascular Repercussions in Puromycin Aminonucleoside-induced Nephrotic Syndrome 2018 BioMed Research International Volume 2018, Article ID 3609645, 10 pages. doi:10.1155/2018/3609645.

22.  Liliana Simões-Silva, Ricardo Araujo, Manuel Pestana, Isabel Soares-Silva, Benedita Sampaio-Maia 2018 The microbiome in chronic kidney disease patients undergoing hemodialysis and peritoneal dialysis Pharmacological Research doi: 10.1016/j.phrs.2018.02.011

 1.Soares-Silva, I., Andrade, R.P., Schuller, D., Casal, M. 2003. Exploring genetic tools for the over-expression of the lactate permease Jen1p of Saccharomyces cerevisiae: constitutive expression in S. cerevisiae and heterologous expression in Pichia pastoris. Yeast, 20. Book of Abstracts. XXI^st Yeast Genetics and Molecular Biology Meeting. Gothenburg, Sweden.

2.Paiva, S., Soares-Silva, I., Kötter, P., Entian, K.-D., Casal, M. 2003. The disruption of JEN1 from Candida albicans impairs the transport of lactate. Yeast, 20. Book of Abstracts. XXI^st Yeast Genetics and Molecular Biology Meeting. Gothenburg, Sweden.

3.Oliveira, J, Soares-Silva, I, Fokkema, I., Gonçalves, A., Cabral, A, Diogo, L., L. Galán, L., Guimarães, A., Fineza, I., den Dunnen, J.T.,Santos, R. 2007. Silent exonic substitution in POMGNT1 promotes exon skipping in a CMD patient. Neuromuscular Disorders vol. 17 Nº. 9-10. 12^th International Congress of the World Muscle Society. Giardini Naxos – Taormina, Italy.

4.Oliveira, J., Soares-Silva, I., Jorge, P., Vieira, E., Oliveira, M., Santos, R. 2007. Widening the LAMA2 mutation spectrum in MDC1A patients: new mutations including gross deletions. Neuromuscular Disorders vol. 17 Nº. 9-10. 12^th International Congress of the World Muscle Society. Giardini Naxos – Taormina, Italy.

5.Quelhas-Santos J., Fernandes-Cerqueira C., Soares-Silva I., Serrão M.P., Simões-Silva L., Ferreira I., Gaião S., Pinho L., Carvalho C., Coentrão L., Pestana M. 2010. Inverse relationship between urinary renalase levels and renal dopaminergic activity in kidney transplant recipients. NDT Plus; 3: iii26-iii274

6.Quelhas-Santos, J., Serrão, P., Fernandes-Cerqueira, C., Simões-Silva, L., Soares-Silva, I., Sizova, D., Sampaio-Maia, B., Desir, G., Pestana, M., 2011. Renal dopaminergic activity is increased in the renalase knockout mouse model, Journal of Hypertension, Vol. 29, e-Supplement A e103

7.Diallinas G, Soares-Silva I, Sá-Pessoa J, Myrianthopoulos V, Mikros E, Casal M, Identification of a Substrate Translocation Trajectory in the Inward-Facing Conformation of the Monocarboxylate/H⁺ Symporter Jen1 2011 Biophysical Journal 100 (3) Pages: 246-246 Suppl 1

8.Simões-Silva, L., Moreira-Rodrigues, M., Quelhas-Santos, J., Fernandes-Cerqueira, C., Pestana, M., Soares-Silva, I., Sampaio-Maia, B. 2012. Assessment of guanylin peptides system in hypertensive obese mice, Journal of Hypertension. Vol. 30, e-Supplement A, e100-101

9.Simões-Silva, L., Moreira-Rodrigues, M., Quelhas-Santos, J., Fernandes-Cerqueira, C., Pestana, M., Soares-Silva, I., Sampaio-Maia, B. 2012 Renal and intestinal guanylin peptides system in a mouse model of diet-induced obesity, NDT, Vol 27, Issue supplii330-ii332

10. Quelhas-Santos, J., Serrão, P., Soares-Silva, I., Tang, L. Desir, G., Sampaio-Maia, B, Pestana, M., 2012 Effects of recombinant renalase on renal dopamine activity in the renalase knockout mouse model, NDT Vol 27, Issue suppl 2  ii330-ii332

11. Ferreira, S., Simões-Silva, L., Soares-Silva, I., Sousa, J., Santos-Araújo, C., Sampaio-Maia, B.  2012 Colonização oral por Staphylococcus em doentes renais crónicos em diálise peritoneal."
Rev Port Estomatol Med Dent Cir Maxilofac 53 : e2.

12. Silva, S Simões-Silva, L., Soares-Silva, I., Sousa, J., Santos-Araújo, C., Sampaio-Maia, B.  2012 Colonização fúngica oral em doentes renais crónicos em diálise peritoneal.  Rev Port Estomatol Med Dent Cir Maxilofac 53, e16-e17.

13. Correia, Inês; Simões-Silva, Liliana; Sousa, Maria João; Soares-Silva, Isabel; Barbosa, Joana; Sampaio-Maia, Benedita;  Avaliação da colonização oral protozoária em doentes renais crónicos em diálise peritonea Rev Port Estomatol Med Dent Cir Maxilofac. 2013;54(Supl.1).e28.

14. Silva, Liliana Simões; Sousa, Maria João; Araújo, Carla Santos; Pestana, Manuel; Silva, Isabel Soares; Maia, Benedita Sampaio; Colonização oral por Pseudomonas e Burkholderia em doentes em Diálise Peritoneal. Rev Port Estomatol Med Dent Cir Maxilofac. 2013;54(Supl.1).e30-e31.

15. Silva, Sara; Lopes, Otília Pereira; Silva, Liliana Simões; Silva, Isabel Soares; Felino, António; Maia, Benedita Sampaio; Comparação da prevalência de Candida sp. entre doentes em Diálise Peritoneal e Hemodiálise Rev Port Estomatol Med Dent Cir Maxilofac. 2013;54(Supl.1).e31.

16. M. Sarmento-Dias, C. Santos-Araújo, R. Poínhos, I. Soares Silva, L. Simões Silva, M. Sousa, F. Correia, M. Pestana, Fgf-23 blood levels predict peritoneal daily phosphate removal in patients on peritoneal dialysis Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association Volume: 29 Suppl 3 Pages: iii223-iii232 Published: 2014-May

17. Silva, Liliana Simões; Ferreira, Susana, Sousa, Maria João; Araújo, Carla Santos; Pestana, Manuel; Silva, Isabel Soares; Maia, Benedita Sampaio; Peritoneal dialysis: infectious agents or normal microbiota 2015; Nephrol. Dial. Transplant.  30 (suppl 3): iii547-iii548.

18..O.P. Lopes, L.S. Simões, J.C. Sousa, M. Tabaio, M.J. Sousa, C.S. Araujo, S. Sampaio, A.C. Braga, A. Felino, M. Pestana, I.S. Silva, B.S. Maia. The impact of hemodialysis or peritoneal dialysis on oral health status in endstage renal disease patients. Euro-Perio. London, UK. June 3-6. 2015. The impact of hemodialysis or peritoneal dialysis on oral health status in endstage renal disease patients. Journal of Clinical Periodontology, 42 (S17): 200.

1.  Benedita Sampaio-Maia, Liliana Simões-Silva, Manuel Pestana, Ricardo Araujo, Isabel Soares-Silva, The role of the gut microbiome on chronic kidney disease. Adv Appl Microbiol. 2016;96:65-94. 

 Poster communications

1.Paiva, S., Soares-Silva, I., Kötter, P., Entian, K.-D., Casal, M. The disruption of JEN1 from Candida albicans impairs the transport of lactate. 13º Congresso Nacional de Bioquímica, Lisboa, 2002.

2.Soares-Silva, I., Andrade, R.P., Casal, M. Heterologous expression of the lactate permease – Jen1p of Saccharomyces cerevisiae in Pichia pastoris. 13º Congresso Nacional de Bioquímica, Lisboa, 2002.

3.Soares-Silva, I., Paiva, S., Kötter P., Entian, Karl-Dieter, Casal M. The disruption of JEN1 from Candida albicans impairs the transport of lactate. MICRO´03, Tomar, 2003.

4.Soares-Silva, I., Schuller, D., Andrade, R. P., Baltazar, F., Cássio F., Casal M. 2003 Functional expression of the lactate permease Jen1p of Saccharomyces cerevisiae in Pichia pastoris. MICRO´03, Tomar, 2003.

5.Soares-Silva, I., Andrade, R., Schuller, D., Casal M. Exploring genetic tools for the over-expression of the lactate permease Jen1p of Saccharomyces cerevisiae: constitutive expression in S. cerevisiae and heterologous expression in Pichia pastoris. XXI^st International Conference on Yeast Genetics and Molecular Biology, Göteborg, Sweden, July 7-12th, 2003.

6.Paiva, S., Soares-Silva, I., Kötter P., Entian, Karl-Dieter, Casal M. The disruption of JEN1 from Candida albicans impairs the transport of lactate. XXI^st International Conference on Yeast Genetics and Molecular Biology, Göteborg, Sweden, July 7-12th, 2003.

7.Soares-Silva, I., Schuller, D., Andrade, R. P., Casal M. Exploring genetic tools for the overexpression of the lactate permease JEN1p of Saccharomyces cerevisiae. XXXI Jornadas de Genética, Oeiras, 2004.

8.Soares-Silva, I., Paiva, S., Kötter P., Entian, Karl-Dieter, Casal M. Cloning of the lactate permease from Candida albicans. XXXI Jornadas de Genética, Oeiras, 2004.

9.Soares-Silva, I., Diallinas, G., Casal, M. Functional analysis of a conserved domain located between helices 7 and 8 of the lactate permease Jen1p in Saccharomyces cerevisiae. Yeast Genetics and Molecular Biology Meeting, University of Washington, Seattle, July 27-August 1, 2004.

10. Soares-Silva, I., Foskolou, Z. and Casal, M. Study of Debaryomyces hansenii Jen1p homologs by heterologous expression in Saccharomyces cerevisiae. MICRO'07 BIOTEC'07-XXXIII JPG, Lisboa, 2007.

11. Oliveira, J., Soares-Silva, I., Jorge, P., Vieira, E., Oliveira, M., Santos, R., Widening the LAMA2 mutation spectrum in MDC1A patients: new mutations including gross deletions, 12^th International Congress of the World Muscle Society, Taormina, Italy, 2007.

12. Oliveira, J., Soares-Silva, I., Fokkema, I., Gonçalves, A., Cabral, A., Diogo, L., Galán, L., Guimarães, A., Fineza, I., den Dunnen, J.T., Santos, R., Silent exonic substitution in POMGNT1 promotes exon skipping in a CMD patient, 12^th International Congress of the World Muscle Society, Taormina, Italy, 2007.

13. Soares-Silva, I., Foskolou, Z., Diallinas, G., and Casal, M., The Jen1p homologs in yeasts: a functional/structural approach on the transport of carboxylic acids across the plasma membrane, 2008 Yeast Genetics and Molecular Biology Meeting, Toronto, Canada, 2008.

14. Soares-Silva, I., Foskolou, Z., Diallinas, G., and Casal, M., Structure/function studies on yeast carboxylate permeases, 26^th Small Meeting on Yeast Transport and Energetics (SMYTE), Braga, Portugal, 2008.

15. Bessa, D., Pacheco, A., Almeida, E., Moreira, R., Soares-Silva, I., Casal, M., Queirós, O. Estratégias para a produção de ácidos carboxílicos numa estirpe de Saccharomyces cerevisiae seleccionada com capacidade melhorada de crescimento em glicerol bruto derivado de biodiesel. III Jornadas de Ciências do ISCS-N, 2009.

16. Soares-Silva, I., Foskolou, Z., Diallinas, G., and Casal, M., Identification of a new domain of the lactate/pyruvate:H+ symporter subfamily critical for distinguishing mono- and di-carboxylate permeases, 24^th International Yeast Genetics and Molecular Biology Conference,  Manchester, United Kingdom, 2009.

17. Soares-Silva, I, Diallinas, G., Casal, M. The lactate permease from Saccharomyces cerevisiae and the lactose permease from Escherichia coli share a common structure that extends to the substrate binding pocket, MicroBiotec 09, Vilamoura, 2009.

18. Quelhas-Santos J., Fernandes-Cerqueira C., Soares-Silva I., Serrão M.P., Simões-Silva L., Ferreira I., Gaião S., Pinho L., Carvalho C., Coentrão L., Pestana M. Inverse relationship between urinary renalase levels and renal dopaminergic activity in kidney transplant recipients. XLVII ERA-EDTA Congress - II DgfN Congress, 25-28 June, Munich, Germany, 2010.

19. Diallinas, G,  Soares-Silva, I, Sá-Pessoa, J, Myrianthopoulos, V, Mikros E, Casal, M. Identification of a substrate translocation trajectory in the inward-facing conformation of the monocarboxylate/H+ symporter Jen1 Biophysical Society 55^th Annual Meeting , 5-9 March, Baltimore, USA, 2011.

20. Simões-Silva, L. Quelhas-Santos, J. Moreira-Rodrigues, M. Soares-Silva, I. Sampaio-Maia, B. Expressão génica da guanilina, uroguanilina e do receptor guanilato ciclase-C no cólon de ratinhos com diabetes tipo 2, IV Jornadas de Análises Clínicas e Saúde Pública, 27-28 de Maio, Porto, 2011.

21. Sá-Pessoa, J,  Soares-Silva, I, , Myrianthopoulos, V, Mikros E, Diallinas, G, Casal, M. Identification of amino acid residues critical for substrate translocation in lactate permease Jen1p of Saccharomyces cerevisiae,  Microbiotec 2011, 01-03 Dez, Braga,  2011.

22. Simões-Silva, L., Moreira-Rodrigues, M., Quelhas-Santos, J., Fernandes-Cerqueira, C., Pestana, M., Soares-Silva, I., Sampaio-Maia, B. Renal and intestinal guanylin peptides system in a mouse model of diet-induced obesity, 49th ERA-EDTA Congress, Paris France, May 24-27, 2012.

1. 23.  Quelhas-Santos, J., Serrão, P., Soares-Silva, I., Tang, L. Desir, G., Sampaio-Maia, B, Pestana, M., Effects of recombinant renalase on renal dopamine activity in the renalase knockout mouse model , 49th ERA-EDTA Congress, Paris, France, May 24-27, 2012.

24. Sara Silva MDD; Liliana Simões-Silva MSc; Isabel Soares-Silva PhD; João Sousa; Benedita Sampaio-Maia PhD; Carla Santos-Araújo MD.Colonização fúngica oral em doentes renais crónicos em diálise peritoneal, XXXII Congresso anual SPEMD, 12 e 13 de Outubro, Lisboa, Portugal 2012.

25.  Susana Ferreira, MDD; Liliana Simões-Silva MSc; Isabel Soares-Silva; João Sousa; Carla Santos-Araújo; Benedita Sampaio-Maia Colonização oral por Staphylococcus em doentes renais crónicos em Diálise Peritoneal, XXXII Congresso anual SPEMD, 12 e 13 de Outubro, Lisboa, Portugal 2012.

26. Sara Silva, Otília Lopes, Liliana Simões-Silva, Isabel Soares-Silva, A Felino, Benedita Sampaio-Maia. Comparação da prevalência de Candida sp. entre doentes em Diálise Peritoneal e Hemodiálise.  XXXIII Congresso Sociedade Portuguesa de Estomatologia e Medicina Dentária, Porto. 11 e 12 de Outubro, Porto, 2013.

27. Inês Correia, Liliana Simões-Silva, Maria João Sousa, Isabel Soares-Silva, Joana Barbosa, Benedita Sampaio-Maia. Avaliação da colonização oral protozoária em doentes renais crónicos em diálise peritoneal. XXXIII Congresso Sociedade Portuguesa de Estomatologia e Medicina Dentária, Porto. 11 e 12 de Outubro, Porto, 2013.

28. Liliana Simões-Silva,  Maria João Sousa, Carla Santos Araújo, Manuel Pestana, Isabel Soares-Silva, Benedita Sampaio-Maia, Colonização oral por Pseudomonas e Burkholderia em doentes em Diálise Peritoneal. Comunicação em poster no XXXIII Congresso Sociedade Portuguesa de Estomatologia e Medicina Dentária, Porto, 2013.

29. Liliana Simões-Silva, Susana Ferreira, Sara Silva, Ricardo Araújo, Maria João Sousa, Carla Santos-Araújo, Manuel Pestana, Isabel Soares-Silva, Benedita Sampaio-Maia. Is there a relation between peritonitis causing agents and the oral microbiota of PD patients? 3º Encontro Doutorandos-FMUP, Porto, Portugal 2013.

30. Inês Correia, Joana Barbosa, Liliana Simões-Silva, Maria João Sousa, Manuel Pestana, Carla Santos-Araujo, Isabel Soares-Silva, Benedita Sampaio-Maia. Prevalence of oral protozoa in Portuguese patients undergoing peritoneal dialysis. Portuguese Congress of Microbiology and Biotechnology', Aveiro, 2013.

31. Inês Correia, Joana Barbosa, Liliana Simões-Silva, Maria João Sousa, Manuel Pestana, Carla Santos-Araújo, Isabel Soares-Silva, Benedita Sampaio-Maia. Dialysis effluent protozoa colonization in Portuguese patients undergoing peritoneal dialysis. Portuguese Congress of Microbiology and Biotechnology' Aveiro, 2013.

32. Liliana Simões-Silva, Susana Ferreira, Sara Silva, Ricardo Araújo, Maria João Sousa, Carla Santos-Araújo, Manuel Pestana, Isabel Soares-Silva, Benedita Sampaio-Maia. Is there a relation between peritonitis causing agents and the oral microbiota of PD patients? Portuguese Congress of Microbiology and Biotechnology, Aveiro, 2013.

33. Inês Correia, Joana Barbosa, Liliana Simões-Silva, Maria João Sousa, Manuel Pestana, Carla Santos-Araújo, Isabel Soares-Silva, Benedita Sampaio-Maia. Protozoa colonization in Portuguese population on peritoneal dialysis, 7^th meeting of young researchers of University o Porto, Porto, 2014.

34. M. Sarmento-Dias, C. Santos-Araújo, R. Poínhos, I. Soares Silva, L. Simões Silva, M. Sousa, F. Correia, M. Pestana, Fgf-23 blood levels predict peritoneal daily phosphate removal in patients on peritoneal dialysis, 51st ERA-EDTA congress, Amsterdam, Holland, 2014

35. Sarmento-Dias M, Araújo CS, Poinhos R, Soares-Silva I, Sousa MJ, Correia F, Pestana M, Volume overload predicts vascular calcification in peritoneal dialysis patients, 15th Congress of the international Society for Peritoneal Dialysis, Madrid, Spain, 2014

36. L. Simões-Silva, S. Ferreira, S. Silva, R. Araujo, M.J. Sousa, C. Santos-Araujo, M. Pestana, I. Soares-Silva, B. Sampaio-Maia, The role of human microbiota and peritoneal dialysis related infections, 2nd World Congress on Targeting Microbiota, Paris, France, October 16-17, 2014.

37. Liliana Simões-Silva, Susana Ferreira, Sara Silva, Ricardo Araujo, Maria João Sousa, Carla Santos-Araújo, Manuel Pestana, Isabel Soares-Silva, Benedita Sampaio-Maia. Searching for peritonitis causing agents in the microbiota of Peritoneal Dialysis patients: analysis of possible routes of infection. 4th I3S Annual Meeting, Póvoa de Varzim. 2014.

38. M. Sarmento-Dias, C. Santos-Araújo, R. Poínhos, I. Soares Silva, L. Simões Silva, M. Sousa, M. Pestana, F. Correia. Phase Angle – a technique to assess nutritional status. 4th I3S Annual Meeting, Póvoa de Varzim. 2014.

39. M. Sarmento-Dias, C. Santos-Araújo, R. Poínhos, I. Soares Silva, L. Simões Silva, M. Sousa, F. Correia, M. Pestana. Volume overload predicts vascular calcification in peritoneal Patients. 4th I3S Annual Meeting, Póvoa de Varzim. 2014.

40. M. Sarmento-Dias, C. Santos-Araújo, R. Poínhos, I. Soares Silva, L. Simões Silva, M. Sousa, F. Correia, M. Pestana. Serum phosphate and FGF-23 blood levels independently predict peritoneal daily phosphate removal in patients on peritoneal dialysis. 4th I3S Annual Meeting, Póvoa de Varzim. 2014.

41. Simões-Silva L., Pestana M., Sampaio-Maia B., Soares-Silva I., Comparison between two Staphylococcus identification methods. 4º Encontro de Doutorandos FMUP, Porto. 2014.

42. L. Simões-Silva, M. Pestana, B. Sampaio-Maia, I. Soares-Silva. API 32 STAPH versus sequencing of DnaJ gene in Staphylococcus identification from PD patients. Winter Science Club INEB 2014. Porto.

1. 43.  L. Simões-Silva, S. Ferreira, M. João Sousa, C. Santos-Araújo, M. Pestana, I. Soares-Silva, B. Sampaio-Maia.colonization in saliva, nasal cavity and catheter exit-site of PD patients. Winter Science Club INEB 2014. Porto.

44. M. Sarmento-Dias, C. Santos-Araújo, R. Poínhos, I. Soares Silva, L. Simões Silva, M. Sousa, F. Correia, M. Pestana "Fibroblast growth factor 23 (FGF-23) in patients on peritoneal dialysis: relationship with peritoneal transport and cardiovascular outcome" - 14 November 2014 - Baxter Investigator Grant Holder's Scientific Evening 2014 - Philadelphia, USA 

45. Liliana Simões-Silva, Susana Ferreira, Sara Silva, Ricardo Araujo, Maria João Sousa, Carla Santos-Araújo, Manuel Pestana, Isabel Soares-Silva, Benedita Sampaio-Maia “Searching for peritonitis causing agents in the microbiota of Peritoneal Dialysis patients: analysis of possible routes of infection”  V SIMPÓSIO TÉCNICO DE ANATOMIA PATOLÓGICA, 13 e 14 de março, 2015, Porto, Portugal 

46. L. Simões Silva, S. Ferreira, M.J. Sousa, C. Santos-Araújo , M. Pestana , I. Soares-Silva, B. Sampaio-Maia “Assessment of Staphylococcus colonization in PD patients” 25th European Congress of Clinical Microbiology and Infectious Diseases (ECCMID 2015), 25-28 April, 2015, Copenhagen Denmark.

47. L. Simões Silva, M. Pestana, B. Sampaio-Maia, I. Soares-Silva “Comparison between two Staphylococcus identification methods” 25th European Congress of Clinical Microbiology and Infectious Diseases (ECCMID 2015), 25-28 April, 2015, Copenhagen Denmark.

48. Silva, Liliana Simões; Ferreira, Susana, Sousa, Maria João; Araújo, Carla Santos; Pestana, Manuel; Silva, Isabel Soares; Maia, Benedita Sampaio; Peritoneal dialysis: infectious agents or normal microbiota 2015; 52nd ERA-EDTA congress May 28th-31st, London, UK.

49. O.P. Lopes, L.S. Simões, J.C. Sousa, M. Tabaio, M.J. Sousa, C.S. Araujo, S. Sampaio, A.C. Braga, A. Felino, M. Pestana, I.S. Silva, B.S. Maia. 2015 The impact of hemodialysis or peritoneal dialysis on oral health status in endstage renal disease patients. Euro-Perio. London, UK. June 3-6.

50. L. Grenho, L. Simões-Silva, B. Sampaio-Maia, M. Pestana, M.P. Ferraz, F.J. Monteiro, I. Soares-Silva, 2016 Establishing an in vitro haemodialysis tunnelled cuffed catheters-model system, Biofilms 7 meeting, Porto, Portugal, June 26-28.

Oral communications

1.Soares-Silva, I., Schuller, D., Andrade, R. P., Casal, M. Permease de lactato - JEN1 de Saccharomyces cerevisiae: expressão constitutiva em S. cerevisiae e sobre-expressão em Pichia pastoris. 10as Jornadas de Biologia de Leveduras Professor N. van Uden. Faro, 2002.

2.Soares-Silva, I., Paiva, S., Kötter^, P., Entian^, K.-D., Casal, M. Identification and characterization of the lactate permease from Candida albicans. 11as Jornadas de Biologia de Leveduras Professor N. van Uden. Bragança, 2003.

3.Soares-Silva, I., Andrade, R., Schuller, D., Casal M. Exploring genetic tools for the over-expression of the lactate permease Jen1p of Saccharomyces cerevisiae: constitutive expression in S. cerevisiae and heterologous expression in Pichia pastoris. XXI Small Meeting on Yeast Transport and Energetics (SMYTE). Bonn, Germany, August 29th-September 1st, 2003.

4.Paiva, S., Soares-Silva, I., Kötter P., Entian, K.-D., Casal M. The disruption of JEN1 from Candida albicans impairs the transport of lactate. XXI Small Meeting on Yeast Transport and Energetics (SMYTE). Bonn, Germany, August 29th-September 1st, 2003.

5.Soares-Silva, I., Schuller, D., Andrade, R. P., Baltazar, F., Cássio F., Casal M. Functional expression of the lactate permease Jen1p of Saccharomyces cerevisiae in Pichia pastoris. 12as Jornadas de Biologia de Leveduras Professor N. van Uden. Aveiro, 2004.

6.Paiva, S., Devaux, F., Barbosa, S., Soares-Silva, I., Jacq, C., Casal, M. Ady2p is essential for the acetate permease activity in the yeast Saccharomyces cerevisiae, 22^nd Small Meeting on Yeast Transport and Energetics (SMYTE), Azores, Portugal 2004.

7.Soares-Silva, I., Diallinas, G., Casal, M., A conserved domain located between helices 7 and 8 of the Saccharomyces cerevisiae Jen1p is involved in substrate recognition, 23^rd Small Meeting on Yeast Transport and Energetics (SMYTE), New Dehli, India, 2005.

8.Soares-Silva, I. ,Diallinas, G. and Casal, M. A conserved domain located between helices 7 and 8 of the Saccharomyces cerevisiae Jen1p is involved in substrate recognition. MICRO'05 - BIOTEC'05, Póvoa de Varzim, 2005.

9.Dias, C., Oliveira, J., Costa, E., Silva, J., Martins, M., Fortuna, A. M., Sousa, A. B., Soares-Silva, I., Kay, T., Santos, H., Medeira, A., Cordeiro, I., Lourenço, T., Nunes, L., Santos, R., Reis-Lima, M. The first Portuguese report on clinical characterization and mutation analysis of the NIPBL gene in 30 patients with Cornelia de Lange Syndrome. 11ª Reunião Anual da Sociedade Portuguesa de Genética Humana, Porto, 2007.

10. *Casal, M., Paiva, S. and Soares-Silva, I. Transport of carboxylic acids in yeast. MICRO'07 BIOTEC'07-XXXIII JPG, Lisboa, 2007.

11. Soares-Silva, I., Paiva, S., Diallinas, G., Casal, M., Structural analysis by comparative modelling of the Saccharomyces cerevisiae lactate permease Jen1p, 3^rd Conference on Physiology of Yeasts and Filamentous Fungi – PYFF3", Helsinquia, Finlândia, 2007.

12. Soares-Silva, I., Paiva, S., Diallinas, G., Casal, M., Study of the Saccharomyces cerevisiae lactate permease Jen1p conserved sequence ³⁷⁹NXX[S/T]HX[S/T]QDXXXT³⁹¹ by comparative modelling, 25^th Small Meeting on Yeast Transport and Energetics (SMYTE), Arraial d’Ajuda, Brasil, 2007.

13. Soares-Silva, I., Barata, B., Diallinas, G. and Casal, M. Identification of a new domain of the lactate/pyruvate:H+ symporter subfamily critical for distinguishing mono- and di-carboxylate permeases, XVII Jornadas de Biologia de Leveduras “Professor Nicolau  van Uden, Braga, 2009.

14. Barata, B., Foskolou, Z., Casal, M., Soares-Silva, I. Study of Debaryomyces hansenii Jen1p homologs by heterologous expression in Saccharomyces cerevisiae, XVII Jornadas de Biologia de Leveduras “Professor Nicolau van Uden, Braga, 2009.

15. Bessa, D., Pacheco, A., Almeida, E., Moreira, R., Soares-Silva, I., Casal, M., Queirós, O. Metabolic engineering of Saccharomyces cerevisiae towards lactic and citric acids production from raw glycerol, XVII Jornadas de Biologia de Leveduras “Professor Nicolau van Uden, Braga, 2009.

16. Barata, B., Foskolou, Z., Bessa, D., Pacheco, A., Queirós, O., Casal, M., Soares-Silva, I. Study of Jen1p homologs by heterologous expression in Saccharomyces cerevisiae, MicroBiotec 09, Vilamoura, 2009

17. Sá-Pessoa J.,Soares-Silva I. ,Diallinas G., Casal M. Identification of amino acid residues critical for distinguishing mono- and dicarboxylate permeases in the lactate/pyruvate:H+ symporter subfamily, XXXV Jornadas Portuguesas de Genética, Braga, 2010

18. Sá-Pessoa J.,Soares-Silva I. ,Diallinas G., Casal M Identification of amino acid residues critical for distinguishing mono- and di-Carboxylate substrates in JEN1, XVIII Jornadas de Biologia de Leveduras "Professor Nicolau van Uden" 18 e 19 de Junho, Lisboa, Portugal, 2010

19. J. Quelhas-Santos, P. Serrão, C. Fernandes-Cerqueira, L. Simões-Silva, I. Soares-Silva, D. Sizova, B. Sampaio-Maia, G. Desir, M. Pestana Renal dopaminergic activity is increased in the renalase knockout mouse model, 21^st European Meeting on Hypertension and Cardiovascular Prevention, Milan, Italy 2011

20. Simões-Silva, L., Moreira-Rodrigues, M., Quelhas-Santos, J., Fernandes-Cerqueira, C., Pestana, M., Soares-Silva, I., Sampaio-Maia, B. Assessment of guanylin peptides system in hypertensive obese mice Journal of Hypertension 22nd European Meeting on Hypertension and Cardiovascular Protection, London ,UK, April 26-29, 2012

21. Inês Correia, Joana Barbosa, Liliana Simões-Silva, Maria João Sousa, Manuel Pestana, Carla Santos-Araújo, Isabel Soares-Silva, Benedita Sampaio-Maia. Dialysis effluent protozoa colonization in Portuguese patients undergoing peritoneal dialysis.Portuguese Congress of Microbiology and Biotechnology' 2013, Aveiro.

22. Liliana Simões-Silva, Susana Ferreira, Sara Silva, Ricardo Araujo, Maria João Sousa, Carla Santos-Araújo, Manuel Pestana, Isabel Soares-Silva, Benedita Sampaio-Maia Searching for peritonitis causing agents in the normal microbiota of Peritoneal Dialysis patients: analysis of possible routes of infection, ECCMID 2014, 24th European Congress of Clinical Microbiology and Infectious Diseases, 10-13 May, Barcelona, Spain, 2014

23. Sarmento-Dias M, Araújo CS, Poínhos R, Soares-Silva I, Sousa MJ, Pestana M, Correia F. Ângulo de fase e calcificação vascular em doentes a realizar diálise peritoneal" Décimo Primeiro Congresso da SPCNA, Porto 16 e 17 de Outubro, 2014

24. Sarmento-Dias M, Araújo CS, Poínhos R, Soares-Silva I, Sousa MJ, Correia F Pestana M "Fósforo sérico e níveis de FGF-23 como preditores da remoção de fósforo peritoneal em doentes a realizar diálise peritoneal" Décimo Primeiro Congresso da SPCNA, Porto 16 e 17 de Outubro, 2014

25. Sarmento-Dias M, Araújo CS, Poínhos R, Soares-Silva I, Sousa MJ, Pestana M, Correia F "Evolução do estado nutricional de uma população de doentes renais crónicos a realizar diálise peritoneal" Décimo Primeiro Congresso da SPCNA, Porto 16 e 17 de Outubro, 2014

26. Soares-Silva I., Ribas D., Foskolou Z., Barata B., Bessa D., Paiva S., Queirós O., Casa M. The Debaryomyces hansenii carboxylate transporters Jen1 homologues are functional in Saccharomyces cerevisiae, 33rd SMYTE meeting (Small Meeting on Yeast Transport and Energetics), Lisboa July 21st-24^th, 2015

Underlined: presenting author

* Invited communication