CBMA
Centro de Biologia Molecular e Ambiental
Centre of Molecular and Environmental Biology
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João Miguel Ferreira Rocha | |
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253604403 | |
Assistant Professor/Researcher | |
Sustaining Life - Biodiversity and Functional Ecology | |
Fernanda Cássio. Cláudia Pascoal | |
Universidade do Minho - Campus de Gualtar, Departamento de Biologia - 4710-057, Braga - P |
Ph.D |
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2016 EcoAgriFood: Innovative green products and processes to promote Agri-Food BioEconomy. |
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2015-16 Screening and optimization of protein crystallization in droplet-based multiphase microfluidic bioreactors. On the scope of the research line Biotechnology and Bioengineering for Improved Industrial and Agrifood Processes. Keywords: Chemical engineering, Biochemistry. Bioreactors. Downstream processing, Separation processes, Protein crystallization, Monitoring, Production and Optimization of crystallization conditions, Protein separation and purification, Micro- and Mesoreactors for protein crystallization, Batch and semi-continuous systems, Thermodynamic and kinetic characterization, Microfluidic reactor, Stirred tanks, Lysozyme, Fibrinolytic proteases and Alpha-lactalbumin crystallization, X-ray analysis, Crystal size and Size distribution, Crystallization rate, yield and purity, Sustainability and environmentally friendly biological processes, Valorization of agro-food by-products. 2014 SusFoFlex – Smart and sustainable food packaging utilizing flexible printed intelligence and materials technologies. Development of edible coatings / nanocoatings for fresh-cut fruit and other perishable products, aiming at improving the shelf-life and/or to reduce the amount of external packaging. Microbiological, physicochemical and textural characterization during shelf-life of food models packaged with novel and sustainable materials. Keywords: Packaging; Edible Films and Coatings; Shelf-life. Fresh-cut fruit and vegetables. Persimmons, melons, watermelons, apples, nectarines. Biochemistry, Chemistry, Microbiology, Sensory and Texture Analysis. Statistics. Biopolymers (locust bean gum, soy protein isolates, pectin, pectin encapsulated extracts, sodium alginate); Plasticizers (glycerol, lactic acid, calcium lactate); Bioactive compounds: Antioxidant activity (calcium ascorbate, sodium ascorbate, orange peels extract, ferulic acid, hesperidin), Crosslinking agents (sodium chloride), Antimicrobial agents (potassium sorbate); Type of package (polyethylene terephthalate (PET), polylactic acid). Analytical parameters: Contact angle, Water vapor permeability, Thickness of the films, Color (whiteness, browning index), Weight loss, pH, Total soluble solids, Mechanical properties (firmness), Microbial and Sensory analysis. 2012-2014 Quality improvement of traditional Alentejo dry sausages through the use of low levels of salt, the monitoring of smoking time and the use of mixed starter cultures. Keywords: Molecular Biology, Microbiology and Microbial Identification. Phenotypic screening. Biochemistry and Chemistry. Processing and Technological Development. Sensory and Texture Analysis. Statistics. Analytical Methods Development. PCR, PCR fingerprinting. Extraction and Derivatization of Biogenic Amines, RP-UHPLC-DAD. Dried Sausages, Meat Industry, Fermentation, Production of Starter Cultures. 2011 Microphyte: Metabolic engineering of Chlamydomonas and environmental optimization for the production and release of hydrogen. Keywords: Microbiology, Engineering, Biochemistry, Statistic, Microalgae. 2002-06 Bovine colustrum processing and industrial valorization. Colostrum processing and isolation of compounds of high-added value for their immunological and growth properties, and their application in the agro-food, pharmaceutical and cosmetic industries. Lines of action: 1-R&D (isolation of IgG-1, lactoferrin, lactalbumin, lactoperoxidase, etc). 2-Industrial applications: pharmaceutical, cosmetic, and agro-food. Keywords: Dairy food, Colostrum, Separation Processes, Chromatography, Freeze-drying, Engineering. Administration, Supervision, Management. 2000-02 Development and improvement of analytical methodologies for lipid extraction, separation and identification. Research on the scope of the Ph.D programme. Selective extraction of lipids after enzyme inactivation, washing, purification in a biphasic partition system, and fractionation by solid phase extraction (SPE). Basic and Acidic (Trans)esterification of glycerides and free fatty acids and silylation of sterol esthers and free sterols, and identification and quantification by GLC. Identification and quantification of neutral lipids (SE, FFA, TAG, DAG, MAG and S), glycolipids (MGDG, DGDG, ESG, SG, DPG) and phospholipids (PI, PS, PE, PC, PG, PA, LPC, LPE, LPS, LPI) by NP-HPLC-ELSD and NP-HPLC-ESI-MS-MS2. Separation and quantification of sterols (cholesterol, cholestanol, brassicasterol, campesterol, stigmasterol, sitosterol, among others) via GLC, after sillylation. Research under PhD programme. Keywords: Lipids, Biochemistry, Chemistry, Analytical Methods Development. Maize, Rye, Wheat, Sourdough, Dough, Bread. 2000 Development and improvement of analytical methodologies for carbohydrate extraction, separation and identification. Research on the scope of the Ph.D programme. Selective extraction, reduction, neutralization, and methylation of oligosaccharides and quantification by GLC (and, partially, by GLC-MS) and monosaccharides by GLC. Quantification of neutral sugars by GLC (after several steps including dialysis, acid hydrolysis, purification, reduction, neutralization and acetylation or methylation) and uronic acid (spectrophotometry), in the fractions of mono-, oligo-and polysaccharides. Research under Ph.D programme. Keywords: Carbohydrates, Biochemistry, Chemistry, GLC, Analytical Methods Development. Maize, Rye, Wheat, Sourdough, Dough, Bread. 1998-2011 Microbiological and lipid profiles of broa: contributions for the characterization of a traditional portuguese bread. Research on the scope of the Ph.D programme. Field 1: Microbiology, Analytical Methods, Microbiological Profile, Fermentation, Classical Microbiology (yeasts, molds, Gram-positive cocci, and Gram-negative and Gram-positive bacilli). Phenotypic screening. Microbial Identification: API and MIDI (GLC) systems. Field 2: Chemistry, Biochemistry, Analytical Methods, Processing and Technological Improvement, Breadmaking. Chemical composition and physical analysis of food. Biochemical parameters (Lipids, Proteins, Amino acids and Carbohydrates). Storage and shelf-life. Texture/Rheological measurements. Sensory evaluation and scoring by a trained sensory panel. Technological baking tests. Correlation between baking technological parameters and chemical and sensory properties. Microbiological and Chemical parameters of water. Field 3: Chemistry, Biochemistry, Lipids. Analytical Methods Development: HPLC, LC-ESI-MS-MS2, GLC. Matrices: Maize, Rye, Wheat, Sourdough, Dough, Bread, Water. 1997 Fed-batch culture and production of lipids by microorganisms (Rhodotorula gracilis). Monitoring of biomass by UV-visible spectrophotometry and by dry weight, extraction of the total lipids, methylation (BF3 and H2SO4) and analysis by GLC of the FAME's. Study of the effect of pH, glucose concentration, agitation and different level of nitrogen sources. Keywords: Microbiology, Biochemistry, Chemistry, Fermentation, Engineering, Monitoring yeast and lipid production, GLC. 1996 Monitoring of baker’s yeast production (Saccharomyces cerevisiae) in a glucose fed-batch culture. Keywords: Microbiology, Engineering, Biochemistry, Chemistry. Fermentation. Optimization of yeast production. Instrumentation and Control. Monitoring (pH, N2, O2 and CO2). Baking, Baker's Yeast. 1995-96 Food quality: physico-chemical and microbiological quality control of chocolates, syrups and similar food products. Keywords: Microbiology, Chemistry. Quality control. Analytical control. |
Lorite, G.S., Rocha, J.M., Miilumäki, N., Saavalainen, P., Selkälä, T., Morales-Cid, G., Gonçalves, M.P., Pongrácz, E., Rocha, C.M.R., Toth, G. (2016) Evaluation of physiochemical/microbial properties and life cycle assessment (LCA) of PLA-based nanocomposite active packaging. LWT – Food Science and Technology 75, 305–315. doi: 10.1016/j.lwt.2016.09.004. Rocha, J.M., Malcata, F.X. (2016) Microbial ecology dynamics during Portuguese broa sourdough. Journal of Food Quality 39, (6), 634–648. Doi: 10.1111/jfq.12244 Rocha, J.M.* (2016) Composition and function of individual microbial biofilm matrix components: a key element to its control. EC Chemistry 2, (2), 153–157. Laranjo, M., Gomes, A., Agulheiro-Santos, A.C., Potes, M.E., Cabrita, M.J., Garcia, R., Rocha, J.M., Roseiro, L.C., Fernandes, M.J., Fraqueza, M.J., Elias, M. (2017) Impact of salt reduction on biogenic amines, fatty acids, microbiota, texture and sensory profile in traditional blood dry-cured sausages. Food Chemistry 218, 129-136. Rocha, J.M.* (2016) On the historical background of bovine colostrum. ECronicon Nutrition 4, (6), 980–981. Rocha, J.M.*, Elias, M.N. (2016) Quality improvement of traditional dry fermented sausages based on innovative technological strategies. Bioaccent Online Journal Nutrition 2, (3), 016, 1–4. Rocha, J.M.* (2016) Microencapsulation of probiotic bacteria. ECronicon Microbiology 3, (4), 480–481. Rocha, J.M.*, Malcata, F.X., Balcão, V.M. (2016) Extra-virgin olive oil: the importance of authentication and quality control. International Journal of Nutritional Science and Food Technology 2, (2), 70–72. Laranjo, M., Gomes, A., Agulheiro-Santos, A.C., Potes, M.E., Cabrita, M.J., Garcia, R., Rocha, J.M., Roseiro, L.C., Fernandes, M.J., Fraqueza, M.J., Elias, M. (2016) Characterisation of “Catalão” and “Salsichão” Portuguese traditional sausages with salt reduction. Meat Science 116, 34–42. doi: 10.1016/j.meatsci.2016.01.015. Rocha, J.M.*, Malcata, F.X. (2015) Behavior of the complex micro-ecology in maize and rye flour and mother-dough for broa throughout storage. Journal of Food Quality 39, 218–233. doi: 10.1111/jfq.12183. Rocha, J.M., Kalo, P.J., Malcata, F.X. (2012) Fatty acid composition of non-starch and starch neutral lipid extracts of portuguese sourdough bread. Journal of the American Oil Chemists’ Society 89, (11), 2025–2045. doi: 10.1007/s11746-012-2110-2. Rocha, J.M., Malcata, F.X. (2012) Microbiological profile of maize and rye flours, and sourdough used for the manufacture of traditional Portuguese bread. Food Microbiology 31, 72–88. doi: 10.1016/j.fm.2012.01.008. Rocha, J.M., Kalo, P.J., Malcata, F.X. (2012) Composition of neutral lipid classes and content of fatty acids throughout sourdough breadmaking. European Journal of Lipid Science and Technology 114, (3), 294–305. doi: 10.1002/ejlt.201100208. Rocha, J.M., Kalo, P.J., Malcata, F.X. (2011) Neutral lipids in free, bound and starch lipid extracts of flours, sourdough and portuguese sourdough bread, determined by NP-HPLC-ELSD. Cereal Chemistry 88, (4), 400–408. doi: 10.1094/CCHEM-11-10-0157. Rocha, J.M., Kalo, P.J., Malcata, F.X. (2010) Neutral lipids in non-starch lipid and starch lipid extracts from portuguese sourdough bread. European Journal of Lipid Science and Technology 112, 1138–1149. doi: 10.1002/ejlt.201000101. Rocha, J.M., Kalo, P.J. Ollilainen, V., Malcata, F.X. (2010) Separation and identification of neutral cereal lipids by normal phase high-performance liquid chromatography, using evaporative light-scattering and electrospray mass spectrometry for detection. Journal of Chromatography A 1217, 3013–3025. doi: 10.1016/j.chroma.2010.02.034. Kalo, P.J., Ollilainen, V., Rocha, J.M., Malcata, F.X. (2006) Identification of molecular species of simple lipids by normal phase liquid chromatography-positive electrospray tandem mass spectrometry, and application of developed methods in comprehensive analysis of low erucic acid rapeseed oil lipids. International Journal of Mass Spectrometry 254, 106–121. doi: 10.1016/j.ijms.2006.05.022. Rocha, J.M., Malcata, F.X. (1999) On the microbiological profile of traditional Portuguese sourdough. Journal of Food Protection 62, (12), 1416–1429. ISSN: 0362-028X. url: http://www.scopus.com/inward/record.url?eid=2-s2.0-0345201643&. |
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EXTENDED ABSTRACTS IN RECORD OF PROCEEDINGS AND CHAPTERS IN BOOKS
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Rocha, J.M., Brás, A., Trigueiros, J.J.B.L., Malcata, F.X. (2003) Pão de milho: Caracterização do produto tradicional de produção e melhoramento tecnológico [Bread of maize: Characterization of the traditional product and technological improvement], ESB-UCP (ed.), ISBN:972-98476-9-X, 76 pp. |
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TECHNICAL AND PEDAGOGICAL PUBLICATIONS IN REFEREED NATIONAL JOURNALS Carvalho, A.P., Duarte, A., Rocha, J.M., Beirão, E., Ramalhosa, M. J., Carona, M. E., Reis, L., Deleure-Matos, C. (2005) Uso de calculadoras gráficas no ensino da química experimental: medição de valores de pH em misturas ácido-base [Using a calculator-based laboratory device designed to undergraduate experimental chemistry course: acid-base titration and pH determination]. Química [Chemistry], Boletim da Sociedade Portuguesa de Química [Bulletin of the Portuguese society of chemistry] 99, 21–24.
TECHNICAL PUBLICATIONS Rocha, J.M., Carvalho, A.P., Baptista, M.F., Barroso, M.F. Delerue-Matos, C. (2006) Manual de apoio às aulas teórico-práticas de Química Geral II [Manual of support to the exercises and problems – General Chemistry II course]. Escola Superior de Biotecnologia, Universidade Católica Portuguesa, Porto, Portugal, 58 pp.
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ORAL COMMUNICATIONS Rocha, J.M. (2017) Opening speech of the Workshop Rótulo Ecológico da União Europeia [European Union Ecolabel]. Universidade do Minho [University of Minho], Braga, Portugal, 31st May. Rocha, J.M. (2017) Ecolabels towards reduction of environmental impacts through life-cycle approach. Opportunities to improve EU Ecolabels. Seminar under the scope of the Master’s courses in Molecular Genetics, Ecology, Applied Biochemistry and Biofisics and Bionanosystems. Departamento de Biologia (DB), Escola de Ciências – Universidade do Minho (UM) [Department of Biology, School of Sciences – University of Minho], Braga, Portugal. 8th March 2017. Rocha, J.M., Lorite, G.S., Miilumäki, N., Saavalainen, P., Selkälä, T., Morales-Cid, G., Gonçalves, M.P., Pongrácz, E., Toth, G., Rocha, C.M. (2016) Novel polylactic acid (PLA)-based active packaging with incorporation of nanoparticles and its performance throughout shelf-life of fresh-cut fruit. XIX Congresso Nacional de Bioquímica [XIX National Congress of Biochemistry], Sociedade Portuguesa de Bioquímica (SPB) [Portuguese Society of Biochemistry], Guimarães, Portugal, December 8-10, O3/05, S3: Plant cell biology and Biotechnology, pp. 31. Laranjo, M., Gomes, A., Agulheiro-Santos, A.C., Potes, M.E., Cabrita, M.J., Garcia, R., Rocha, J.M., Roseiro, L.C., Fernandes, M.J., Fraqueza, M.J., Elias, M. (2016) Effect of salt reduction on the safety and stability of traditional blood dry-cured sausages. IX Simpósio Internacional do Porco Mediterrânico [IX International Symposium on the Mediterranean Pig], Portalegre, Portugal, November 3-5, OC-1402.
Rocha, J.M. (2011) Microbiological and lipid profiles of broa: contributions for the characterization of a traditional portuguese bread. Public presentation of the seminar course. Instituto Superior de Agronomia [Superior School of Agriculture], Universidade de Lisboa [University of Lisbon] (ISA-UL), Lisbon, Portugal, October 24.
Rocha, J.M., Sousa, A.M., Santos, J., Alves, R., Gonçalves, R., Oliveira, M.B., Gonçalves, M.P., Rocha, C.M. (2016) On the correlation between physicochemical properties of agar and its performance as edible coating and film to enhance shelf-life of fresh fruit. XIX Congresso Nacional de Bioquímica [XIX National Congress of Biochemistry], Sociedade Portuguesa de Bioquímica (SPB) [Portuguese Society of Biochemistry], Guimarães, Portugal, December 8-10.
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2017. Rocha, J.M., Barroso, M.F. Supervision of a master’s student. Master thesis entitled Development of electrochemical genosensors for detection of food allergens, food and environmental pathogenic microorganisms and genetically modified organisms. Departamento de Biologia (DB) – Universidade do Minho (UM) [Department of Biology – University of Minho], Braga, Portugal. 2017. Rocha, J.M., Barroso, M.F., Ruiz, S.C, Pingarrón, J.M. Supervision of an Erasmus master’s student. Development of electrochemical genosensors for detection of food adulterations. Departamento de Química Analítica [Department of Analytical Chemistry], Facultad de Ciencias Químicas [Faculty of chemical sciences], Universidad Complutense de Madrid [Complutense University of Madrid], Madrid, Spain. Departamento de Biologia (DB) – Universidade do Minho (UM) [Department of Biology – University of Minho], Braga, Portugal.
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