CBMA

The mainstream interests relate with the yeast cell wall and extracellular matrix (ECM), which detailed molecular characterization is under way in Saccharomyces cerevisiae and Candida albicans. This work is in line with the more general aim of unveiling a putative novel morphogenic Hedgehog-like signalling pathway in lower eukaryotes, as suggested by the experimental evidence from the last decade relating to the GUP1 gene. This was originally discovered by our work in association with defects on glycerol transport. It actually encodes an MBOAT 0-acyltransferase involved in a vast diversity of cellular processes, and very similar to the higher Eukaryotes HHAT proteins.

Key words: Yeast, cell wall, plasma membrane, extracellular matrix, signalling, Hedgehog

 A second line of interest derives from the previous work on glycerol transport and metabolism regulation in yeasts, S. cerevisiae and other less conventional ones. The biotechnological implications of glycerol production and utilization in yeasts are manifold. Presently, our group dedicates to improve S. cerevisiae ability to consume glycerol for biomass production or, alternatively, for ethanol production. This metabolic engineering will allow the utilization of the biodiesel industry glycerol-rich waste by re-introducing it in the biofuel production cycle.

The work is presently being developped in close collaboration with Rogélio Brandão from the NUPEB, Federal Unviersity of Ouro Preto, Brazil, and under the finantial support of a Bolsa Pesquisador Visitante Especial - Programa Ciências sem Fronteiras - CAPES.

Further work aims at developing a yeast-based methodology for the utilization of the wastes from sugar-cane bio-ethanol industry in Brazil for the production of glycerol for frther use in the production in loco of biodiesel. This work is under the finantial support of FAPESP, Brazil, and is being done in cooperation with the company FERMENTEC from Piracicaba, SP, Brazil.

Key words: yeast, glycerol, biodiesel, bioethanol, biofuels, energetic sustainability.

TEAM  
   
 
Joana Tulha - PhD Student - PhD Programme in Mol. & Environm. Biol., CBMA, UMinho
Giulia Cazzanelli - PhD Student - Marie Curie ITN - GLYCOPHARM, CBMA, UMinho

Pedro Ferraz - PhD Student - PhD Programme in Microbiology  /FCT grantee, CBMA, UMinho

  
 Aureliano Cunha - PhD student CAPES at NUPEB/UFOP, Brasil

Lucas, C., Ferreira, C., Cazzanelli, G., Franco-Duarte, R., Tulha, J. (2016) Yeast Gup1(2) proteins are homologues of the Hedgehog morphogens acyl transferases HHAT(L): facts and implications. J. Dev. Biol. 4: 33.

Faria-Oliveira, F., Carvalho, J., Ferreira, C., Hernáez, M.L., Gil, C., Lucas, C. (2015) Quantitative differential proteomics of yeast extracellular matrix: there is more to it than meets the eye. BMC Microbiol. 15(1): 271.

Dušková, M., Ferriera, C., Lucas, C., Sychrová, H. (2015) Two glycerol uptake systems contribute to the high osmotolerance of Zygosaccharomyces rouxii. Mol. Microbiol. 97(3): 541-559.

Faria-Oliveira, F., Carvalho, J., Belmiro, C.L.R., Ramalho, G., Pavão, M., Lucas, C., Ferreira, C. (2015) Elemental biochemical analysis of the polysaccharides in the extracellular matrix of the yeast Saccharomyces cerevisiae. J. Basic Microbiol. 54:1–10.

Faria-Oliveira, F., Carvalho, J., Belmiro C.L.R., Martinez-Gomariz, M., Hernaez, ML, Pavão, P., Gil, C., Lucas, C.and Ferreira, C. (2014) Methodologies to generate, extract, purify and fractionate yeast ECM for analytical use in proteomics and glycomics. BMC Microbiol. 14(1): 244.

Ferreira D, Nobre A, Silva ML, Faria-Oliveira F, Tulha J, Ferreira C, Lucas C. (2013) XYLH encodes a xylose/H⁺ symporter from the highly related yeast species Debaryomyces fabryi and Debaryomyces hansenii. FEMS Yeast Res. 2013 doi: 10.1111/1567-1364.12061. [Epub ahead of print]

Tulha, J., Faria-Oliveira, F., Costa C., Lucas, C., Ferreira, C. (2012) Saccharomyces cerevisiae Gup1p is required for a programmed cell death process. BMC Microbiology 12(1): 80.

Tulha, J., Lima, A., Lucas, C. and Ferreira, C. (2010) Saccharomyces cerevisiae glycerol/H⁺ symporter STL1p is essential for cold/near-freeze and freeze stress adaptation. A simple recipe with high biotechnological potential is given. Microb. Cell Fact. 9(1): 82-89. 

Ferreira, C., Silva, S., Faria-Oliveira, F., Pinho, E., Henriques, M. and Lucas, C. (2010) Candida albicans virulence and drug-resistance requires the O-acyltransferase GUP1p. BMC Microbiol. 15: 238-252. 

Kayingo, G., Martins, C., Andrie, R., Neves, L., Lucas, C. and Wong, B. (2009) A permease encoded by STL1 is required for active glycerol uptake by Candida albicans. Microbiology 155: 1547-57. 

Sant'Ana, G., Paes, L., Paiva, A., Fietto, L., Totola, A., Tropia, M.J. Giunchetti, D., Lucas, C., Fietto, J., Brandão, R. and Castro, I. (2009) Protective effect of ions against cell death induced by acid stress in Saccharomyces. FEMS Yeast Res. 9: 701-12. 

da Silva, S., Calado, S., Lucas, C. and Almeida-Aguiar, C. (2008) Unusual phenotypic properties of the halotolerant yeast Candida nodaensis killer toxin, CnKT. Microbiol. Res. 163: 243-51.

Ferreira, C. and Lucas, C. (2008) The yeast O-acyltransferase GUP1p interferes in lipid metabolism with direct consequences on the sphingolipid-sterol ordered domains integrity/assembly. Biochim. Biophys. Acta 1778 (11): 2648-53.

Ferreira, C. and Lucas, C. (2007) Glucose repression over Saccharomyces cerevisiae glycerol/H⁺ symporter gene STL1 is overcome by high temperatures. FEBS Lett., 581: 1923-1927. 

Ferreira, C., Silva, S., van Voorst, F., Aguiar, C., Kielland-Brandt, M., Brandt, A. and Lucas, C. (2006) Absence of GUP1p in Saccharomyces cerevisiae results in a defective cell wall composition, assembly, stability and morphology. FEMS Yeast Res., 6: 1027-1038.

Ferreira, C., van Voorst, F., Martins, A., Neves, L., Oliveira, R., Kielland-Brandt, M. C., Lucas, C. and Brandt, A. (2005) A member of the sugar transporter family, STL1p is the glycerol/H⁺ symporter in Saccharomyces cerevisiae. Mol. Biol. Cell 16: 2068–2076. 

Oliveira, R. and Lucas, C. (2004) Expression studies of GUP1 and GUP2, genes involved in glycerol active uptake in Saccharomyces cerevisiae, using semi-quantitative RT-PCR. Curr. Genet. 46: 140-146. 

Neves, L., Lages, F. and Lucas C. (2004) New insights on glycerol transport in Saccharomyces cerevisiae. FEBS Lett., 565: 160-162. 

Neves, L., Oliveira, R. and Lucas C. (2004) Yeast orthologues associated with glycerol transport and metabolism. FEMS Yeast Res. 5: 51-62. 

Silva-Graça, M. and Lucas, C. (2003) Physiological studies on the long-term adaptation to salt-stress in the extremely halotolerant yeast Candida versatilis CBS4019 (syn. C. halophila) FEMS Yeast Res., 3: 247-260. 

Silva-Graça, M., Neves, L. and Lucas, C. (2003) Outlines for the definition of halotolerance/ halophily in yeasts: Candida versatilis (halophila) CBS4019 as the archetype? FEMS Yeast Res., 3: 347-362. 

Oliveira, R., Lages, F., Silva-Graça, M., and Lucas, C. (2003) Fps1p channel is the mediator of the major part of the glycerol passive diffusion in Saccharomyces cerevisiae: artefacts and re-definitions. Biochim. Biophys. Acta. Biomemb. 1613: 57 – 71. 

Aguiar, C. and Lucas, C. (2000) Yeasts Killer/sensitivivity phenotypes and halotolerance. Food Technol. Biotechnol., 38 (1): 39-46. 

Hölst, B., Lunde, C., Lages, F., Oliveira, R., Lucas, C. and Kielland-Brandt, M. (2000) GUP1 and its close homologue GUP2, encoding multi-membrane-spanning proteins involved in active glycerol uptake in Saccharomyces cerevisiae. Mol. Microbiol, 37: 98-107.

Nobre, A. Lucas, C. and Leão, C. (1999) Transport and utilization of hexoses and pentoses in the halotolerant yeast Debaryomyces hansenii. Appl. Environm. Microbiol., 65 (8): 3594-3598. 

Lages, F., Silva-Graça, M. and Lucas, C. (1999) Active glycerol transport is a mechanism underlying halotolerance in yeasts: a study of 42 species. Microbiol., 145: 2577-2586. 

Neves, M. L., Oliveira, R. P. and Lucas, C. M. (1997) Metabolic flux response to osmotic stress in the halotolerant yeast Debaryomyces hansenii. Microbiol., 143: 1133-1139. 

Lages, F. and Lucas, C. (1997) Contribution to the physiological characterization of glycerol active uptake in Saccharomyces cerevisiae. Bioch. Biophys. Acta. Bioenergetics 1322: 8-18. 

Sutherland, F.C.W., Lages, F., Lucas, C., Luyten, C., Albertyn, J., Hohmann, S., Prior B. and Kilian, S. G. (1997) Characteristics of Fps1-dependent and –independent glycerol transport in Saccharomyces cerevisiae. J. Bacteriol., 179 (24): 7790-7795. 

Oliveira, R.P., Lages, F. and Lucas, C. (1996) Isolation and characterisation of mutants from the halotolerant yeast Pichia sorbitophila defective in H⁺/glycerol symport activity. FEMS Microbiol. Lett., 142: 147-153. 

Lages, F. and Lucas, C. (1995) Characterization of a glycerol/H⁺ symport in the halotolerant yeast Pichia sorbitophila. Yeast, 11: 111-119. 

Lucas, C., da Costa, M. and van Uden, N. (1990) Osmoregulatory active sodium-glycerol co-transport in the halotolerant yeast Debaryomyces hansenii. Yeast, 6: 187-191. 

Lucas, C. and van Uden, N. (1988) Interconversion and glucose-induced inactivation of glucose transport systems in Candida shehatae. J. Basic Microbiol., 28: 639-643. 

Lucas, C. and van Uden, N. (1986) Transport of hemicellulose monomers in the xylose fermenting yeast Candida shehatae. Appl. Microbiol. Biotechnol., 23: 491-495. 

Lucas, C. and van Uden, N. (1985) The temperature profiles of growth, thermal death and ethanol tolerance of the xylose fermenting yeast Candida shehatae. J. Basic Microbiol., 25: 547-550. 

Creation of a new research infrasturcture of Minho University:  IB-S Institute for Science and Innovation on Bio-Susutainability - financed by ON2 programme, CCDRN/QREN. This will be perational in the end of 2014.

2013/16 OLEOLEV: FAPESP funded R&D project. Creation of a yeast-based biorefinery process for the utilization of sugar cane-based bio-etahnol industry in Brasil. Project developed in collaboration with FERMENTEC.

Lucas, C., Ferreira, C., Cazzanelli, G., Franco-Duarte, R., Tulha, J. (2016) Yeast Gup1(2) proteins are homologues of the Hedgehog morphogens acyl transferases HHAT(L): facts and implications. J. Dev. Biol. 4: 33.

Azenha, M., Lucas, C., Granja, J.L., Carlos-Alves, I., Guimarães, E. (2016) Glycerol resulting from biodiesel production as an admixture for cement-based materials: an experimental study. Eu. J. Environm. Civil Eng. doi.org/10.1080/19648189.2016.1177603

Pinheiro, M.L., Serôdio, P. Pinho, J.C., Lucas, C. (2016) The role of Social Capital towards Resource Sharing in collaborative R&D projects: evidences from the 7th Framework Programme. Int. J. Proj. Managm. 34: 1519-1536.

Pinheiro, M.L., Lucas, C., Pinho, J.C. (2015) The outset of U-I R&D relationships: The specific case of Biological Sciences. Eur. J. Innov. Manag. 18(3): 282-306.

Pinheiro, M.L., Lucas, C., Pinho, J.C. (2015) Social Network Analysis as a new methodological tool to understand University-cooperation. Int. J. Innov. Manag. 19(1): 1550013. 

Faria-Oliveira, F., Carvalho, J., Ferreira, C., Hernáez, M.L., Gil, C., Lucas, C. (2015) Quantitative differential proteomics of yeast extracellular matrix: there is more to it than meets the eye. BMC Microbiol. 15(1): 271.

Faria-Oliveira, F., Carvalho, J., Belmiro, C.L.R., Ramalho, G., Pavão, M., Lucas, C., Ferreira, C. (2015) Elemental biochemical analysis of the polysaccharides in the extracellular matrix of the yeast Saccharomyces cerevisiae. J. Basic Microbiol. 54:1–10.

Dušková, M., Ferriera, C., Lucas, C., Sychrová, H. (2015) Two glycerol uptake systems contribute to the high osmotolerance of Zygosaccharomyces rouxii. Mol. Microbiol. 97(3): 541-559.

Conceição, L., Saraiva, M., Diniz, R., Oliveira, J., Barbosa, G., Alvarez, F., Correa, L., Mezadri, H., Coutrim, M., Afonso, R., Lucas, C., Castro, I., Brandão, R. (2015) Biotechnological potential of yeast isolates from cachaça – the Brazilian spirit. J. Ind. Microbiol. & Biotechnol. 42(2):237-46.

Faria-Oliveira, F., Carvalho, J., Belmiro C.L.R., Martinez-Gomariz, M., Hernaez, ML, Pavão, P., Gil, C., Lucas, C.and Ferreira, C. (2014) Methodologies to generate, extract, purify and fractionate yeast ECM for analytical use in proteomics and glycomics. BMC Microbiol. 14(1): 244.

Ferreira D, Nobre A, Silva ML, Faria-Oliveira F, Tulha J, Ferreira C, Lucas C (2013) XYLH encodes a xylose/H⁺ symporter from the highly related yeast species Debaryomyces fabryi and Debaryomyces hansenii. FEMS Yeast Res. 2013 doi: 10.1111/1567-1364.12061. [Epub ahead of print]

Pinheiro, M., Pinho, J.C., Lucas, C. (2012) Examining University-Industry interactions from the perspective of relationship marketing and business networks. IMP2012 – Università Cattolica del Sacro Cuore – Roma, Italy www.impgroup.org/paper_view.php?viewwww.impgroup.org/paper_view.php?viewPaper=7841

Tulha, J., Faria-Oliveira, F., Costa C., Lucas, C., Ferreira, C. (2012) Saccharomyces cerevisiae Gup1p is required for a programmed cell death process. BMC Microbiology 12(1): 80.

Tulha, J., Lima, A., Lucas, C. and Ferreira, C. (2010) Saccharomyces cerevisiae glycerol/H⁺ symporter STL1p is essential for cold/near-freeze and freeze stress adaptation. A simple recipe with high biotechnological potential is given. Microb. Cell Fact. 9(1): 82-89. 

Ferreira, C., Silva, S., Faria-Oliveira, F., Pinho, E., Henriques, M. and Lucas, C. (2010) Candida albicans virulence and drug-resistance requires the O-acyltransferase GUP1p. BMC Microbiol. 15: 238-252. 

Kayingo, G., Martins, C., Andrie, R., Neves, L., Lucas, C. and Wong, B. (2009) A permease encoded by STL1 is required for active glycerol uptake by Candida albicans. Microbiology 155: 1547-57. 

Sant'Ana, G., Paes, L., Paiva, A., Fietto, L., Totola, A., Tropia, M.J. Giunchetti, D., Lucas, C., Fietto, J., Brandão, R. and Castro, I. (2009) Protective effect of ions against cell death induced by acid stress in Saccharomyces. FEMS Yeast Res. 9: 701-12. 

da Silva, S., Calado, S., Lucas, C. and Almeida-Aguiar, C. (2008) Unusual phenotypic properties of the halotolerant yeast Candida nodaensis killer toxin, CnKT. Microbiol. Res. 163: 243-51.

Ferreira, C. and Lucas, C. (2008) The yeast O-acyltransferase GUP1p interferes in lipid metabolism with direct consequences on the sphingolipid-sterol ordered domains integrity/assembly. Biochim. Biophys. Acta 1778 (11): 2648-53.

Ferreira, C. and Lucas, C. (2007) Glucose repression over Saccharomyces cerevisiae glycerol/H⁺ symporter gene STL1 is overcome by high temperatures. FEBS Lett., 581: 1923-1927. 

Ferreira, C., Silva, S., van Voorst, F., Aguiar, C., Kielland-Brandt, M., Brandt, A. and Lucas, C. (2006) Absence of GUP1p in Saccharomyces cerevisiae results in a defective cell wall composition, assembly, stability and morphology. FEMS Yeast Res., 6: 1027-1038.

Ferreira, C., van Voorst, F., Martins, A., Neves, L., Oliveira, R., Kielland-Brandt, M. C., Lucas, C. and Brandt, A. (2005) A member of the sugar transporter family, STL1p is the glycerol/H⁺ symporter in Saccharomyces cerevisiae. Mol. Biol. Cell 16: 2068–2076. 

Oliveira, R. and Lucas, C. (2004) Expression studies of GUP1 and GUP2, genes involved in glycerol active uptake in Saccharomyces cerevisiae, using semi-quantitative RT-PCR. Curr. Genet. 46: 140-146. 

Neves, L., Lages, F. and Lucas C. (2004) New insights on glycerol transport in Saccharomyces cerevisiae. FEBS Lett., 565: 160-162. 

Neves, L., Oliveira, R. and Lucas C. (2004) Yeast orthologues associated with glycerol transport and metabolism. FEMS Yeast Res. 5: 51-62. 

Silva-Graça, M. and Lucas, C. (2003) Physiological studies on the long-term adaptation to salt-stress in the extremely halotolerant yeast Candida versatilis CBS4019 (syn. C. halophila) FEMS Yeast Res., 3: 247-260. 

Silva-Graça, M., Neves, L. and Lucas, C. (2003) Outlines for the definition of halotolerance/ halophily in yeasts: Candida versatilis (halophila) CBS4019 as the archetype? FEMS Yeast Res., 3: 347-362. 

Oliveira, R., Lages, F., Silva-Graça, M., and Lucas, C. (2003) Fps1p channel is the mediator of the major part of the glycerol passive diffusion in Saccharomyces cerevisiae: artefacts and re-definitions. Biochim. Biophys. Acta. Biomemb. 1613: 57 – 71. 

Aguiar, C. and Lucas, C. (2000) Yeasts Killer/sensitivivity phenotypes and halotolerance. Food Technol. Biotechnol., 38 (1): 39-46. 

Hölst, B., Lunde, C., Lages, F., Oliveira, R., Lucas, C. and Kielland-Brandt, M. (2000) GUP1 and its close homologue GUP2, encoding multi-membrane-spanning proteins involved in active glycerol uptake in Saccharomyces cerevisiae. Mol. Microbiol, 37: 98-107.

Nobre, A. Lucas, C. and Leão, C. (1999) Transport and utilization of hexoses and pentoses in the halotolerant yeast Debaryomyces hansenii. Appl. Environm. Microbiol., 65 (8): 3594-3598. 

Lages, F., Silva-Graça, M. and Lucas, C. (1999) Active glycerol transport is a mechanism underlying halotolerance in yeasts: a study of 42 species. Microbiol., 145: 2577-2586. 

Neves, M. L., Oliveira, R. P. and Lucas, C. M. (1997) Metabolic flux response to osmotic stress in the halotolerant yeast Debaryomyces hansenii. Microbiol., 143: 1133-1139. 

Lages, F. and Lucas, C. (1997) Contribution to the physiological characterization of glycerol active uptake in Saccharomyces cerevisiae. Bioch. Biophys. Acta. Bioenergetics 1322: 8-18. 

Sutherland, F.C.W., Lages, F., Lucas, C., Luyten, C., Albertyn, J., Hohmann, S., Prior B. and Kilian, S. G. (1997) Characteristics of Fps1-dependent and –independent glycerol transport in Saccharomyces cerevisiae. J. Bacteriol., 179 (24): 7790-7795. 

Oliveira, R.P., Lages, F. and Lucas, C. (1996) Isolation and characterisation of mutants from the halotolerant yeast Pichia sorbitophila defective in H⁺/glycerol symport activity. FEMS Microbiol. Lett., 142: 147-153. 

Lages, F. and Lucas, C. (1995) Characterization of a glycerol/H⁺ symport in the halotolerant yeast Pichia sorbitophila. Yeast, 11: 111-119. 

Lucas, C., da Costa, M. and van Uden, N. (1990) Osmoregulatory active sodium-glycerol co-transport in the halotolerant yeast Debaryomyces hansenii. Yeast, 6: 187-191. 

Lucas, C. and van Uden, N. (1988) Interconversion and glucose-induced inactivation of glucose transport systems in Candida shehatae. J. Basic Microbiol., 28: 639-643. 

Lucas, C. and van Uden, N. (1986) Transport of hemicellulose monomers in the xylose fermenting yeast Candida shehatae. Appl. Microbiol. Biotechnol., 23: 491-495. 

Lucas, C. and van Uden, N. (1985) The temperature profiles of growth, thermal death and ethanol tolerance of the xylose fermenting yeast Candida shehatae. J. Basic Microbiol., 25: 547-550. 

Tulha, T., Carvalho, J., Armada, R., Faria-Oliveira, F., Lucas, C., Pais, C., Almeida, J., Ferreira, C. (2011) Yeasts, the man’s best friend. in “Scientific, Health and Social Aspects of the Food Industry”, ISBN 979-953-307-283-7 INTECH Open Acecss Publisher. Eds Benjamin Valdez, Roumen Zlatev, Michael Schorr, Instituto de Ingeniería, Universidad Autónoma de Baja California, Mexicali, México.

Abstracts IN proceedings   IN ISI-Web of KnowledgE

Pinheiro,  M.L., Lucas, C., Pinho, J.C., 2014. “Social Network Analysis as a new methodological tool to understand University-Industry cooperation”. Abstract book from KITAB 2014 - Knowledge, Innovation and Technology Across Borders: An Emerging Research Agenda. Lisbon, Portug

Pinheiro, M.L., Lucas, C., Pinho, J.C., 2014. “The role of R&D project outcomes in partner satisfaction and relationship continuity: the case of FP7 cooperation projects”. Abstract book from KITAB 2014 - Knowledge, Innovation and Technology Across Borders: An Emerging Research Agenda. Lisbon, Portugal.

Silva, S., Aguiar, C., Veríssimo, P., Pires, E. and Lucas, C. (2003) The killer toxin of the halotolerant yeast Candida nodaensis. XXIth Yeast Genetics and Molecular Biology Meeting. Gothenburg, Sweden. Yeast, 20: 201.

Neves L. , Lages , F., Oliveira, R., Brandt, A., Kielland-Brandt, M. and Lucas, C. (2003) Saccharomyces cerevisiae glycerol uptake: new insights. XXI Yeast Genetics and Molecular Biology Meeting. Göteborg, Sweden. Yeast, 20: 248.

Ferreira, C., Lucas, C., Brandt, A. and Kielland-Brandt, M. (2003) Saccharomyces cerevisiae GUP1, a gene involved in active glycerol uptake: new phenotypes. XXI Yeast Genetics and Molecular Biology Meeting. Göteborg, Sweden. Yeast, 20: 249.

Oliveira, R., Lages, F., Silva-Graça, M. and Lucas, C. (2003) Fps1p channel is the mediator of the major part of glycerol passive diffusion in Saccharomyces cerevisiae: artefacts and re-definitions. XXI Yeast Genetics and Molecular Biology Meeting. Göteborg, Sweden. Yeast, 20: 249.

Aguiar, C, Veríssimo, P., Pires, E.and Lucas, C. (2001) The Killer toxin from the halotolerant yeast Candida nodaensis. XXth International Conference on Yeast Genetics and Molecular Biology, Prague, Czech Republic. Yeast, 18: 323.

Oliveira R. and C. Lucas (2001) Expression of GUP1 and GUP2, Saccharomyces cerevisiae glycerol active transport genes. XXth International Conference on Yeast Genetics and Molecular Biology, Prague, Czech Republic. Yeast, 18: 90.

Aguiar, C. and Lucas, C. (1999) Correlation between Killer and salt stress resistance phenotypes. XIX Intern. Conference on Yeast Genetics and Molecular Biology, Rimini, Italy. Curr. Genet., 35/3-4: 446.

Sutherland, F.C.W., Lages, F., Lucas, C., Luyten, C., Albertyn, J., Hohmann, S., Prior B. and Kilian, S. G. (1997) Characteristics of Fps1-dependent and –independent glycerol transport in Saccharomyces cerevisiae. XVIII Itern. Conference on Yeast Genetics and Molecular Biology, Stellenbosch, SA. Yeast, 13: 150.

Oliveira, R. and Lucas, C. (1997) Saccharomyces cerevisiae genomic lybrary screening in search for the gene responsible for inductive active glycerol uptake. XVIII Iternational Conference on Yeast Genetics and Molecular Biology, Stellenbosch, South Africa. Yeast, 13: 138.

Oliveira, R. P. and Lucas, C. (1995) Classical Genetics studies in the halotolerant yeast Pichia sorbitophila: as background for mutagenesis strategy. XVII International Conference on Yeast Genetics and Molecular Biology, Lisbon, Portugal. Yeast, 11: 449.

Lages, F., Neves, L. and Lucas, C. (1995) Constitutive glycerol active transport systems: Two compared models for halotolerance and salt toxicity. XVII International Conference on Yeast Genetics and Molecular Biology, Lisbon, Portugal. Yeast, 11: 444.

Lages, F. and Lucas, C. (1994) Glycerol/ H⁺ symport in the halotolerant yeast Pichia sorbitophila: Characterization of an electrogenic active transport system. 12^th SMYTE. Folia Microbiol., 39: 528-529.

Lucas C (2016) Behind the wall: exploring the glycobiology of yeasts extracellular matrix. GLYCOPHARM Final Workshop, Madrid, Spain.

Lucas C. (2015) Multicellularity challenges in yeast. Programas de Pós-Graduação em Biotecnologia e Ciências Biológicas, NUPEB (Núcleo de Pesquisas em Ciências Biológicas), Universidade Federal de Ouro Preto, Brasil.

Faria-Oliveira, F., Ferreira, C., Lucas, C. (2015) Saccharomyces cerevisiae extracellular matrix: there is more to it than meets the eye. 33th SMYTE, Lisbon, Portugal.

Lucas, C. (2015) Glycoside structure of extracellular matrix from yeast. GlycoBiology Summer School, Inter-ITN Workshop, Braga, Portugal.

Lucas C. (2013) Roupa nova de pano velho. FERMETEC – Soluções Tecnológicas Industriais, Piracicaba, SP, Brasil

Lucas C. (2012) Glicero(bio)refinaria: Roupa nova de pano velho. Programas de Pós-Graduação em Biotecnologia e Ciências Biológicas, NUPEB (Núcleo de Pesquisas em Ciências Biológicas), Universidade Federal de Ouro Preto, Brasil.

Ferreira, C., Silva, S., Faria-Oliveira, F., Henriques, M., Lucas, C. (2009) Importance of CaGUP1p, an O-acyltransferase, in azoles resistance, hyphal development and adhesion, invasion and biofilm formation of Candida albicans. Conference in Yeast Genetics & Molecular Biology, Manchester, UK.

Lucas, C. (2008) Glycerol homeostasis, a newborn concept on yeast physiology. XXXVII Reunião Annual da Sociedade Brasileira de Bioquímica e Biologia Molecular e XI Congresso da Associação Pan-Americana de Bioquímica e Biologia Molecular, Águas de Lindóia, Brasil.

Lucas C. (2007) Glycerol homeostasis, a newborn concept on yeast physiology. 25^th SMYTE, Arraial d’Ajuda, Brasil.

Lucas, C. (2007) Histórias do glicerol em leveduras. Conferência van Uden - XV Jornadas de Biologia de Leveduras “Prof. N. van Uden”, Póvoa do Varzim, Portugal.

Lucas C. (2007) Histórias do glicerol e dos seus genes. NUPEB, Univ. Federal de Ouro Preto, Brasil. Conferência inserida no workshop “Bioquímica e Fisiologia de Microrganismos – Aspectos Básicos e Aplicados”.

Lucas, C. (2003) Yeasts in heavy stress. MICRO’2003, Symposium on Microbial Physiology, Tomar, Portugal. 

Joana Tulha

PhD Student (2013/16) SFRH/ BD/76025/2011

Subject

Co-supervision of Célia Ferreira from CBMA, UMinho

Giulia Cazzanelli

PhD Student (2013/16) - Marie Curie ITN GLYCOPHARM 

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The Lab team in 2016

From left:

Me
Giulia Cazzanelli (PhD student)
Joana Tulha (PhD student)
Pedro Ferraz (PhD student)

The Lab team in 2014

From left: