CBMA

Major societal challenges of the 21st century are Human Health and Wellbeing and the Pressure on Natural Resources. My goal, aligned with the CBMA strategic program and Horizon 2020, is to conduct cutting-edge research through a problem-oriented approach enabling breakthrough innovations, new tools and strategies impacting health promotion and environmental sustainability. I have established an independent research line studying proteasome-mediated modulation of cell death induced by chemotherapeutic agents, and collaborate with senior researchers in the apoptosis field, applied to cancer research. More recently, I started a CBMA effort focused on environmental monitoring and drug discovery.

Rego A, Cooper K, Snider J, Hannun Y, Costa V, Côrte-Real M and Chaves SR Acetic acid induces Sch9-dependent translocation of Isc1p from the endoplasmic reticulum into mitochondria Biochim Biophys Acta. 2018 Feb 27;1863(6):576-583

Pereira H, Oliveira CSF, Castro L, Preto A, Chaves SR*, Côrte-Real M* (*equal contributor) Yeast as a tool to explore cathepsin D function (2015) Microbial Cell , Vol. 2, No. 7, pp. 225 - 234

Oliveira CSF, Pereira H, Alves S, Castro L, Baltazar F, Chaves SR, Preto A, Côrte-Real M Cathepsin D protects colorectal cancer cells from acetate-induced apoptosis through autophagy-independent degradation of damaged mitochondria (2015) Cell Death Dis. 2015 Jun 18;6:e1788.

Rego A, Duarte AM, Azevedo F, Sousa MJ, Côrte-Real M and Chaves SR Cell wall dynamics modulate acetic acid-induced apoptotic cell death of Saccharomyces cerevisiae (2014) Microbial Cell 1(9):303-14

Cunha D., Cunha R., Côrte-Real M. and Chaves SR Cisplatin-induced cell death in Saccharomyces cerevisiae is programmed and rescued by proteasome inhibition DNA Repair (Amst). (2013) Jun 1;12(6):444-9. doi: 10.1016/j.dnarep.2013.02.005. Epub 2013 Apr 18.

Chaves SR*, Rosenblum JS Efficient Nuclear Transport of Structurally Disturbed Cargo: Mutations in a Cargo Protein Switch Its Cognate Karyopherin. PLoS ONE (2011) 6(2): e16846. doi:10.1371/journal.pone.0016846 (*Corresponding author)

Chaves S, Baskerville C, Yu V, Reed SI. Cks1, Cdk1 and the 19S proteasome collaborate to regulate gene-induction-dependent nucleosome eviction in yeast. Mol Cell Biol. (2010) Nov;30(22):5284-94 (Evaluated and recommended by the Faculty of 1000 (Post-publication peer review)).

Martinsson Ahlzén H.S, Liberal V., Grünenfelder B., Chaves S.R., Spruck C.H. and Reed S.I. Cyclin-dependent kinase associated proteins Cks1 and Cks2 are essential during early embryogenesis and for cell cycle progression in somatic cells. Mol Cell Biol. (2008) 28(18):5698-709

Pereira C, Chaves S, Alves S, Salin B, Camougrand N, Manon S, Sousa MJ, Côrte-Real M. Mitochondrial degradation in acetic acid-induced yeast apoptosis: the role of Pep4 and the ADP/ATP carrier Mol Microbiol. (2010) Jun;76(6):1398-410 (Commented at Molecular Microbiology, Micro Commentary by Lawen A. Another piece of the puzzle of apoptotic cytochrome c release, 66(3):553-6)

Chaves S.R. and Blobel G. Nuclear import of Spo12p, a protein essential for meiosis. J Biol Chem. (2001) 276(21), pp: 17712-7

Team member:

FCT Project POCI-01-0145-FEDER-029505 "FunG-Eye– A functional approach to unravel the interaction between fungicide pollution and fungi-mediated ecosystem processes"

CBMA Project "Pestisense– Novel tools and data for the detection and assessment of ecological risks of pesticides"

Norte2020 project NORTE-01-0145-FEDER-000009 "EcoAgriFood: Innovative green products and processes to promote Agri-Food BioEconomy"

PESSOA Project 3746/ 37989UA " Modulation of Bax activity and partner interaction by phosphoregulation"

Strategic Project UID/BIA/04050/2013: CENTRE OF MOLECULAR AND ENVIRONMENTAL BIOLOGY

 PI:

The Lance Armstrong Foundation project # 04 TCY19 "Identification of Molecular Determinants of Cisplatin Resistance"

Team member:

FCT project FCT-ANR/BEX-BCM/0175/2012 "Phosphoregulation of Bax-dependent cell death"

FCT project PTDC/BBB-BQB/1850/2012 “Role of the yeast neutral sphingomyelinase in mitochondria function and iron homeostasis”

FCT project EXPL/BIM-ONC /0056/2012 “Regulation of Bax activation by the oncogene PKC epsilon: An approach to eliminate cancer cells”

FCT project PTDC/BIA-BCM/69448/2006 “Elucidation of ceramide-induced apoptosis: modulation of protein kinase C isoforms”

Collaborator:

FCT project PTDC/AGR-ALI/102608/2008 “Mechanistic insights into acetic acid resistance in food spoilage yeasts: from the experimental model Saccharomyces cerevisiae to Zygosaccharomyces spp”

25- Rego A, Cooper K, Snider J, Hannun Y, Costa V, Côrte-Real M and Chaves SR Acetic acid induces Sch9-dependent translocation of Isc1p from the endoplasmic reticulum into mitochondria Biochim Biophys Acta. 2018 Feb 27;1863(6):576-583 

24- Alves S, Neiri L, Chaves SR, Vieira S, Trindade D, Manon S, Dominguez V, Pintado B, Jonckheere V, Van Damme P, Silva RD, Aldabe R, Côrte-Real M. N-terminal acetylation modulates Bax targeting to mitochondria. Int J Biochem Cell Biol. 2017 Dec 9;95:35-42

23- Barroca M, Rodrigues P, Sobral R, Costa MM, Chaves SR, Machado R, Casal M, Collins T. Antibiotic free selection for the high level biosynthesis of a silk-elastin-like protein. (2016) Sci Rep. Dec 16;6:39329. doi: 10.1038/srep39329

22- Trindade D, Pereira C, Chaves SR, Manon S, Côrte-Real M and Sousa MJ VDAC regulates AAC-mediated apoptosis and cytochrome c release in yeast (2016) Microbial Cell, Vol. 3, No. 10, pp. 369 - 379; DOI: 10.15698/mic2016.10.533

21- Alves S, Castro L, Fernandes MS, Castro P, Priault M, Chaves SR, Moyer MP, Oliveira C, Seruca R, Côrte-Real M, Sousa MJ, Preto A Hotspot mutations in the KRAS oncogene function as positive regulators of autophagy (2015) Oncotargets  Oct 13;6(31):30787-802. doi: 10.18632/oncotarget.5021.

20- Castro L, Alves S, Chaves SR, Costa JL, Soares P, Preto A RAF-1 promotes survival of thyroid cancer cells harboring RET/PTC1 rearrangement independently of ERK activation (2015) Mol Cell Endocrinol, pii: S0303-7207(15)30046-0.

19- Pereira H, Oliveira CSF, Castro L, Preto A, Chaves SR*, Côrte-Real M* (*equal contributor) Yeast as a tool to explore cathepsin D function (2015) Microbial Cell , Vol. 2, No. 7, pp. 225 - 234

18- Oliveira CSF, Pereira H, Alves S, Castro L, Baltazar F, Chaves SR, Preto A, Côrte-Real M Cathepsin D protects colorectal cancer cells from acetate-induced apoptosis through autophagy-independent degradation of damaged mitochondria (2015) Cell Death Dis. 2015 Jun 18;6:e1788.

17- Teixeira V, Medeiros TC, Vilaça R, Pereira AT, Chaves SR, Côrte-Real M, Moradas-Ferreira P, Costa V. Ceramide signalling impinges on Sit4p and Hog1p to promote mitochondrial fission and mitophagy in Isc1p-deficient cells (2015) Cell Signal 27(9):1840-9

16- Rego A, Duarte AM, Azevedo F, Sousa MJ, Côrte-Real M and Chaves SR Cell wall dynamics modulate acetic acid-induced apoptotic cell death of Saccharomyces cerevisiae (2014) Microbial Cell 1(9):303-14

15- Rego A, Trindade D, Chaves SR, Manon S, Costa V, Sousa MJ, Côrte-Real M Insigths from the yeast model system towards the understanding of apoptosis regulation by sphingolipids (2014) FEMS Yeast Research 14 (1), pp. 160-178

14- Sousa M, Duarte AM, Chaves SR, Pacheco A, Leão C, Côrte-Real M, Sousa MJ Genome-wide identification of genes involved in the positive and negative regulation of acetic acid-induced programmed cell death in Saccharomyces cerevisiae (2013) BMC Genomics 14 (1), 838

13- Pacheco A, Azevedo F, Rego A, Santos J, Chaves SR, Côrte-Real M and Sousa MJ C2-phytoceramide perturbs lipid rafts and cell integrity in Saccharomyces cerevisiae in a sterol-dependent manner (2013) PloS one 8 (9), e74240

12- Cunha D., Cunha R., Côrte-Real M. and Chaves S.R. Cisplatin-induced cell death in Saccharomyces cerevisiae is programmed and rescued by proteasome inhibition DNA Repair (Amst). (2013) Jun 1;12(6):444-9. doi: 10.1016/j.dnarep.2013.02.005. Epub 2013 Apr 18.

11- Marques C., Oliveira C.S., Alves S., Chaves S.R., Coutinho O.P., Côrte-Real M., Preto A. Acetate-induced apoptosis in colorectal carcinoma cells involves lysosomal membrane permeabilization and cathepsin D release Cell Death Dis. (2013) Feb 21;4:e507. doi: 10.1038/cddis.2013.29.

10- Pereira HP, Azevedo F Sousa MJ, Chaves SR and Côrte-Real M. The protective role of yeast cathepsin D in acetic acid-induced apoptosis depends on ANT (Aac2p) but not on the voltage-dependent channel (Por1p) FEBS Letters (2013) 587(2):200-5

9- Rego A., Costa A.M., Chaves S.R., Pereira H.P., Sousa M.J., Moradas-Ferreira P., Costa V. and Côrte-Real M. Modulation of mitochondrial outer membrane permeabilization and apoptosis by ceramide metabolism. PLoS ONE (2012) 7(11): e48571. doi:10.1371/journal.pone.0048571

8- Barbosa AD, Graça J, Mendes V, Chaves SR, Amorim MA, Mendes MV, Moradas-Ferreira P, Côrte-Real M, Costa V. Activation of the Hog1p kinase in Isc1p-deficient yeast cells is associated with mitochondrial dysfunction, oxidative stress sensitivity and premature aging. Mech Ageing Dev. (2012) 133(5):317-30.

7- Sousa MJ, Azevedo F, Pedras A, Marques C, Coutinho OP, Preto A, Gerós H, Chaves SR, Côrte-Real M. Vacuole-mitochondrial cross-talk during apoptosis in yeast: a model for understanding lysosome-mitochondria-mediated apoptosis in mammals. Biochem Soc Trans. (2011) Oct;39(5):1533-7

6- Chaves SR*, Rosenblum JS Efficient Nuclear Transport of Structurally Disturbed Cargo: Mutations in a Cargo Protein Switch Its Cognate Karyopherin. PLoS ONE (2011) 6(2): e16846. doi:10.1371/journal.pone.0016846 (*Corresponding author)

5- Vilela-Moura A., Schuller D., Mendes-Faia A., Silva R.D., Chaves S.R., Sousa M.J. and Côrte-Real M. The impact of acetate metabolism on yeast fermentative performance and wine quality: zymological reduction of volatile acidity of grape-musts and wines Appl Microbiol Biotechnol. (2011) Jan;89(2):271-80.

4- Chaves S, Baskerville C, Yu V, Reed SI. Cks1, Cdk1 and the 19S proteasome collaborate to regulate gene-induction-dependent nucleosome eviction in yeast. Mol Cell Biol. (2010) Nov;30(22):5284-94 (Evaluated and recommended by the Faculty of 1000 (Post-publication peer review))

3- Pereira C, Chaves S, Alves S, Salin B, Camougrand N, Manon S, Sousa MJ, Côrte-Real M. Mitochondrial degradation in acetic acid-induced yeast apoptosis: the role of Pep4 and the ADP/ATP carrier Mol Microbiol. (2010) Jun;76(6):1398-410 (Commented at Molecular Microbiology, Micro Commentary by Lawen A. Another piece of the puzzle of apoptotic cytochrome c release, 66(3):553-6)

2- Martinsson Ahlzén H.S, Liberal V., Grünenfelder B., Chaves S.R., Spruck C.H. and Reed S.I. Cyclin-dependent kinase associated proteins Cks1 and Cks2 are essential during early embryogenesis and for cell cycle progression in somatic cells. Mol Cell Biol. (2008) 28(18):5698-709

1- Chaves S.R. and Blobel G. Nuclear import of Spo12p, a protein essential for meiosis. J Biol Chem. (2001) 276(21), pp: 17712-7

Pacheco A, Santos J., Chaves S., Almeida J., Leão C. and Sousa M.J. The emerging role of the Torulaspora Delbrueckii in bread and wine production: Using genetic manipulation to study molecular basis of physiological responses. InTech- Structure and Function of Food Engineering, ISBN 978-953-51-0695-1 

Comunicações orais por convite:

Mammalian and yeast as complementary models in programmed cell death-Advanced course, Minho University, Braga, June 22, 2015:

Yeast as a model system to study mechanisms of apoptosis induced by anti-tumour drugs

==============================

Mammalian and yeast as complementary models in programmed cell death-Advanced course, Minho University, Braga, June 24, 2014:

Yeast as a model system to study mechanisms of apoptosis induced by anti-tumour drugs

==============================

Ciência Falada - Seminar Series, ICVS, Minho University, Braga, June 16, 2014:

Azevedo F., Duarte A.M., Rego A., Sousa M.J., Côrte-Real M. and Chaves S.R. Cell wall dynamics modulate acetic acid-induced apoptotic cell death of Saccharomyces cerevisiae

==============================

10th International Meeting on Yeast Apoptosis, Gotemburg, Sweden, 14-18 May, 2014:

Oliveira C.S.F., Pereira H., Alves S., Chaves S. R., Sousa M.J., Preto A. and Côrte-Real M. The yeast vacuolar protease Pep4p unveils a novel function of the human Cathepsin D during acetate-induced mitochondrial apoptosis in colorectal carcinoma cells. Presented by Manuela Côrte-Real.

==============================

Mammalian and yeast as complementary models in programmed cell death-Advanced course, Minho University, Braga, June 18, 2013:

Yeast as a model system to study mechanisms of apoptosis induced by anti-tumour drugs

==============================

Jornadas do Centro de Física, Minho University, Braga, October 26, 2012:

Biophysical approaches towards the elucidation of molecular mechanisms underlying programmed cell death

==============================

9th International Meeting on Yeast Apoptosis, Rome, Italy, 16-20 September, 2012:

Rego A., Costa A.M., Chaves S., Moradas-Ferreira P., Costa V. and Côrte-Real M. Modulation of mitochondrial outer membrane permeabilization and apoptosis by sphingolipid metabolism. Presented by Manuela Côrte-Real.

==============================

25th International Conference on Yeast Genetics and Molecular Biology, Kortowo-Olsztyn, Poland, 11-16 July, 2011:

Rego A., Costa A.M., Chaves S., Moradas-Ferreira P., Côrte-Real M. and Costa V. Isc1p-dependent modulation of apoptosis induced by acetic acid in yeast. Presented by Vítor Costa

==============================

8th International Meeting on Yeast Apoptosis May 2 - 6, 2011 University of Kent, Canterbury, United Kingdom:

Chaves S, Pereira C, Marques C, Rodrigues A, Salin B, Alves S, Silva R, Gerós H, Coutinho OP, Preto A, Camougrand N, Manon S, Sousa MJ and Côrte-Real M Vacuole-mitochondrial crosstalk during apoptosis induced by acetic acid in yeast. Presented by Manuela Côrte-Real.

==============================

14th Portugaliae Genetica, 17-18 March, 2011, IPATIMUP,Porto:

Chaves S, Pereira C, Marques C, Rodrigues A, Salin B, Alves S, Silva R, Gerós H, Coutinho O, Preto A, Camougrand N, Manon S, Sousa M J and Côrte-Real M. What yeast can tell us about how cells commit suicide? Presented by Manuela Côrte-Real.

==============================

9th International Micology Congress 1-6 August Edinburgh, UK 2010 Session: Programmed Cell Death and Autophagy:

Chaves S, Pereira C, Alves S, Salin B, Camougrand, Manon S, Sousa M J and Côrte-Real M. A non-autophagic role for the vacuole in mitochondrial degradation during acetic acid-induced yeast apoptosis. Presented by Manuela Côrte-Real.

==============================

Autumn 2010 Meeting, University of Nottingham, Jubilee Campus 6-9 September, 2010 Session-Microbial death:

Chaves S, Pereira C, Alves S, Salin B, Camougrand, Manon S, Sousa M J and Côrte-Real M. Acetic acid-induced apoptosis in Saccharomyces cerevisiae. Presented by Manuela Côrte-Real.

==============================

DBio Seminars, Department of Biology, University of Minho, Braga, Portugal, May 27 2009:

Chaves S.R, Yu V.P and Reed S.I. Cks1, Cdk1 and the proteasome collaborate to regulate nucleosome density on yeast chromatin

==============================

Outras comunicações orais
Selected for oral communications:

XIX Congresso Nacional de Bioquímica, December 08-10, 2016, Guimarães, Portugal :

Machado M, Costa AR, Cunha D, Rego A, Côrte-Real M, Chaves SR The mechanism underlying proteasome-mediated sensitivity to the chemotherapeutic agent cisplatin

==============================

11th International Meeting on Yeast Apoptosis, Porto, Portugal September 29th- October 3rd 2015:

Alves S, Castro L, Fernandes MS, Francisco R, Castro P, Priault M, Chaves SR, Moyer MP, Oliveira C, Seruca R, Côrte-Real M, Sousa MJ, Preto A. Colorectal cancer-related mutant KRAS alleles function as positive regulators of autophagy. Presented by Sara Alves.

Castro L, Alves S, Pereira C, Chaves SR, Sousa MJ, Manon S and Côrte-Real M. Yeast as a tool to identify novel kinases involved in regulation of Bax. Presented by Lisandra Castro.

==============================

XX JORNADAS DE BIOLOGIA DE LEVEDURAS “PROFESSOR NICOLAU VAN UDEN” IST, Lisbon, 11-12 July 2014:

Azevedo F, Duarte AM, Rego A, Sousa MJ, Côrte-Real M and Chaves SR Cell wall dynamics modulate acetic acid-induced apoptotic cell death of Saccharomyces cerevisiae

==============================

10th International Meeting on Yeast Apoptosis, Gotemburg, Sweden, 14-18 May, 2014:

-Azevedo F., Duarte A.M., Rego A., Sousa M.J., Côrte-Real M. and Chaves S.R. Cell wall dynamics modulate acetic acid-induced apoptotic cell death of Saccharomyces cerevisiae

-Sousa M., Duarte, A.M., Fernandes T.R., Chaves S.R., Pacheco A., Leão C., Côrte-Real M. and Sousa M.J. Regulation of acetic acid-induced programmed cell death in Saccharomyces cerevisiae: insights from a functional genomic approach. Presented by Maria João Sousa.

==============================

Jornadas de Bioquímica, April 2 2014, UTAD, Vila Real:

Pacheco A.R and Chaves S.R The role of Cks1 protein in apoptosis. Presented by Ana Pacheco

==============================

1st PDBMA symposium, Braga, November 28 2013:

Pereira H.P, Chaves, S.R and Côrte-Real M. The role of Pep4p, the vacuolar yeast protease ortholog of human Cathepsin D, in mitochondria-dependent apoptosis. Presented by Helena Pereira

==============================

XIX JORNADAS DE BIOLOGIA DE LEVEDURAS “PROFESSOR NICOLAU VAN UDEN” FCT/UNL Campus da Caparica, 15-16 June 2012:

Cunha D., Cunha R., Côrte-Real M and Chaves S.R. Characterization of cisplatin-induced cell death in Saccharomyces cerevisiae 

Pacheco, A., Azevedo, F., Chaves, S.R., Côrte-Real M. and Sousa, M.J. C2-phytoceramide induces cell wall defects and sensitivity to osmotic stress in Saccharomyces cerevisiae. Presented by Maria João Sousa.

==============================

XXXV Jornadas de Genética, Universidade do Minho, Campus de Gualtar, Braga, May31-June02 2010, 2010:

Alves, S, Chaves, S, Côrte-Real, M, Preto, A and Sousa, MJ Study of the role of human oncogene KRAS mutations in autophagy regulation using the yeast expression system. Presented by Sara Alves.

==============================

XVII JORNADAS DE BIOLOGIA DE LEVEDURAS “PROFESSOR NICOLAU VAN UDEN” Universidade do Minho, 19-20 June 2009:

Chaves, S, Yu, V and Reed SI Cks1, Cdk1 and the proteasome collaborate to regulate nucleosome density on yeast chromatin

==============================

Comunicações em painel ("poster")

XIX Congresso Nacional de Bioquímica, December 08-10, 2016, Guimarães, Portugal :

Martins V, Amaral L, Bagheri M, Côrte-Real M, Sousa MJ, Chaves SR The role of metabolism in the sensitivity of S. cerevisiae to the anti-cancer agent cisplatin

Rego A, Costa V, Chaves SR and Côrte-Real MCeramide metabolism regulates apoptotic cell death induced by acetic acid via Pkh1p-Ypk1p and Pkh1p-Sch9p pathways. Presented by Manuela Côrte-Real.  

==============================

3rd PDBMA symposium, Braga, November 29th, 2015:

Machado N, Rego A, Castro L, Côrte-Real M and Chaves SR The mechanism underlying proteasome-mediated sensitivity to the chemotherapeutic agent cisplatin. Presented by Nuno Machado.

Rego A, Costa V, Chaves SR and Côrte-Real M Signaling pathways involved in acetic acid-induced apoptosis in Saccharomyces cerevisiae. Presented by António Rego.

Castro L, Alves S, Pereira C, Chaves SR, Sousa MJ, Manon S and Côrte-Real M. Yeast as a model system to characterize Bax regulation by individual kinases. Presented by Lisandra Castro.

==============================

11th International Meeting on Yeast Apoptosis, Porto, Portugal September 29th- October 3rd 2015:

Machado N, Mendes I, Rego A, Castro L, Côrte-Real M and Chaves SR The mechanism underlying proteasome-mediated sensitivity to the chemotherapeutic agent cisplatin. Presented by Nuno Machado.

Rego A, Costa V, Chaves SR and Côrte-Real M Signaling pathways involved in acetic acid-induced apoptosis in Saccharomyces cerevisiae. Presented by António Rego.

Oliveira C.S.F., Pereira H., Alves S., Castro L., Baltazar F., Chaves S.R., Preto A. and Côrte-Real M. Cathepsin D protects colorectal cancer cells from acetate-induced apoptosis through autophagy-independent degradation of damaged mitochondria. Presented by Cláudia Oliveira.

Campos V, Rego A, Cunha R, Sousa MJ, Costa V, Côrte-Real M and Chaves S.R. Signaling Isc1p-regulated cell death: the role of possible downstream regulators. Presented by António Rego.

==============================

XXXIX Jornadas Portuguesas de Genética, Braga, Portugal, 25-27 May, 2015

Campos V, Cunha R, Rego A, Sousa MJ, Costa V, Côrte-Real M and Chaves S.R. Signaling Isc1p-regulated cell death: the role of mitochondrial apoptosis mediators. Presented by Vanessa Campos

==============================

2nd PDBMA Symposium, Braga, Portugal, 27 November, 2014

Campos V, Cunha R, Rego A, Sousa MJ, Costa V, Côrte-Real M and Chaves S.R. Signaling Isc1p-regulated cell death: the role of mitochondrial apoptosis mediators. Presented by Vanessa Campos

Rego A, Pereira H, Sousa MJ, Hannun Y, Costa V, Chaves SR and Côrte-Real M Ceramide mediates acetic acid-induced apoptosis in Saccharomyces cerevisiae. Presented by António Rego

==============================

XVIII Congress of the Portuguese Biochemical Society, Coimbra, December 17-20, 2014

Oliveira CSF , Pereira H, Alves S, Chaves SR, Sousa MJ, Preto A, Côrte-Real M. The yeast vacuolar protease Pep4p unveils a novel function of the human Cathepsin D during acetate-induced mitochondrial apoptosis in colorectal carcinoma cells. Presented by Helena Pereira

Alves S, Castro L, Chaves SR, Sousa MJ, Manon S, Côrte-Real M. Phosphoregulation of Bax-dependent cell death: towards the
identification of new protein kinases as molecular targets for cancer therapy. Presented by Lisandra Castro

==============================
Symposium on Sphingolipids in Health and Disease, IBMC, Porto, Portugal, 18 September, 2014

Pacheco A, Azevedo F, Rego A, Santos J, Chaves S, Côrte-Real M and Sousa MJ. Mechanisms underlying synthetic ceramides cytotoxicity in Saccharomyces cerevisiae. Presented by Maria João Sousa

Campos V, Cunha R, Rego R, Sousa MJ,, Costa V, Chaves S and Côrte-Real M. Signaling Isc1p-mediated cell death: the role of possible upstream and downstream regulators. Presented by Vanessa Campos

==============================

XX JORNADAS DE BIOLOGIA DE LEVEDURAS “PROFESSOR NICOLAU VAN UDEN” IST, Lisbon, 11-12 July 2014:

Castro L., Silva A., Chaves S.R., Sousa, M.J. and Côrte-Real M. Identification of protein kinases/phosphatases involved in the phosphoregulation of Bax-dependent cell death. Presented by Lisandra Castro

==============================

10th International Meeting on Yeast Apoptosis, Gotemburg, Sweden, 14-18 May, 2014:

Castro L., Silva A., Chaves S.R., Sousa, M.J. and Côrte-Real M. Identification of protein kinases/phosphatases involved in the phosphoregulation of Bax-dependent cell death. Presented by Manuela Côrte-Real

==============================

2nd EACR Special Conference on Cell Death in Cancer, Amsterdam, Netherlands, 30 January –1 February 2014:

Oliveira C.S.F., Marques C., Alves S., Chaves S., Côrte-Real M. and Preto A. Acetate induces lysosomal membrane permeabilization, cathepsin D release and impairs autophagy in colorectal carcinoma cells. Presented by Cláudia Oliveira.

==============================

MicroBiotec - Portuguese Congress of Microbiology and Biotechnology, 6-8 December 2013, Aveiro.

Rego A, Costa M, Chaves S, Matmati N, Pereira H, Sousa MJ, Moradas-Ferreira P, Hanun Y, Costa V and Côrte-Real M Ceramide metabolism modulates mitochondrial outer membrane permeabilization and apoptosis. Presented by António Rego.

Pacheco A, Azevedo F, Rego A, Santos J, Chaves S, Côrte-Real M, Sousa MJ C2-Phytoceramide Perturbs Lipid Rafts and Cell Integrity in Saccharomyces cerevisiae in a Sterol-Dependent Manner. Presented by Andreia Pacheco.

Pereira H, Azevedo F, Sousa1 MJ, Chaves S, Côrte-Real M. The importance of mitochondrial function in the role of the yeast Cathepsin D in acetic acid-induced apoptosis. Presented by Helena Pereira.

==============================

1st PDBMA symposium, Braga, November 28 2013

Pacheco A.R. and Chaves S.R. The role of Cks proteins in apoptosis. Presented by Ana Pacheco.

Rego A., Chaves S.R., Pereira H., Sousa M.J., Hannun Y., Costa V. and Côrte-Real M. Ceramide metabolism modulates mitochondrial outer membrane permeabilization and yeast apoptosis. Presented by António Rego

Oliveira C.S.F., Marques C., Alves S., Chaves S.R., Coutinho O.P. Côrte-Real M. and Preto A. Involvement of lysosomal membrane permeabilization and cathepsin D release in colorectal carcinoma cells acetate-induced apoptosis. Presented by Cláudia Oliveira.

==============================

7th International Ceramide Conference, October 20-24, 2013, Montauk, New York, USA:

Rego A, Costa M, Chaves S, Matmati N, Pereira H, Sousa MJ, Moradas-Ferreira P, Hannun Y, Costa V and Côrte-Real M Ceramide metabolism modulates mitochondrial outer membrane permeabilization and apoptosis.presented by António Rego

==============================

21st ECDO Euroconference on Apoptosis on “Cell death: a Biomedical paradigm” Institut Pasteur, 25-28 September 2013, Paris, France:

Pacheco A, Azevedo F, Rego A, Santos J, Chaves S, Côrte-Real M, Sousa MJ C2-Phytoceramide Perturbs Lipid Rafts and Cell Integrity in Saccharomyces cerevisiae in a Sterol-Dependent Manner. Presented by Maria João Sousa

==============================

9th International Meeting on Yeast Apoptosis, Rome, Italy, 16-30 September, 2012:

Cunha D., Cunha R., Côrte-Real, M. and Chaves S.R. Characterization of cisplatin-induced cell death in Saccharomyces cerevisiae

Pereira H, Azevedo F, Sousa MJ, Chaves SR, Côrte-Real M. The yeast CatD protective role in acetic acid-induced apoptosis depends on the yeast ANT (Aac2p) but not on the voltage-dependent channel (Por1p). Presented by Helena Pereira

Alves S., Chaves, S.R., Côrte-Real, M., Preto, A., Sousa, M.J. Unravel the role of KRAS in autophagy control in colorectal carcinoma: Saccharomyces cerevisiae as a model system. Presented by Sara Alves.

Pacheco, A., Azevedo, F., Chaves, S., Côrte-Real M. and Sousa, M.J. C2-phytoceramide induces cell integrity defects and sensitivity to osmotic stress. Presented by Andreia Pacheco

==============================

Porto Cancer Meeting, Porto, 19-20 April, 2012

Alves S., Chaves, S.R., Oliveira, C., Seruca, R., Côrte-Real, M., Sousa, M.J. and Preto, A. The role of KRAS mutations in autophagy regulation in colorectal carcinoma. Presented by Sara Alves.

==============================

MicroBiotec - Portuguese Congress of Microbiology and Biotechnology, Braga, 1-3 December, 2011

Cunha D. and Chaves S.R.Yeast as a model system to study mechanisms regulating cisplatin sensitivity and resistance. Presented by Dulce Cunha

Azevedo, F, Chaves, SR, Johansson, B, Sousa, MJ and Côrte-Real, M. MAPK signaling pathways mediate acetic acid-induced cell death in Saccharomyces cerevisiae. Presented by Flávio Azevedo.

Sousa, M., Chaves, S., Leão, C., Côrte-Real, M. and Sousa, M.J. Genome-wide identification of genes involved in the positive and negative regulation of acetic acid-induced cell death in Saccharomyces cerevisiae. Presented by Marlene Sousa

Pacheco, A., Azevedo, F., Chaves, S., Côrte-Real M. and Sousa, M.J. Role of C2-phytoceramide in mediating cell death in yeast. Presented by Andreia Pacheco

Rego A., Costa A.M., Chaves S., Moradas-Ferreira P., Côrte-Real M. and Costa V. The involvement of spingolipids in apoptosis induced by acetic acid in yeast. Presented by António Rego.

==============================

25th International Conference on Yeast Genetics and Molecular Biology, Kortowo-Olsztyn, Poland, 11-16 July, 2011

Rego A., Costa A.M., Chaves S., Moradas-Ferreira P., Côrte-Real M. and Costa V. Isc1p-dependent modulation of apoptosis induced by acetic acid in yeast. Presented by Vítor Costa

==============================

XXXV Jornadas de Genética, Universidade de Coimbra, Braga, May30-June01 2011:

Cunha D., Côrte-Real, M. and Chaves S.R. Characterization of cisplatin-induced cell death in S. Cerevisiae. Presented by Dulce Cunha

==============================

XVII Congresso Nacional de Bioquímica, Porto, Portugal, 15-17 December, 2010:

Alves S., Chaves, S., Oliveira, C., Seruca, R., Côrte-Real, M., Preto, A. and Sousa,
M.J. The role of human oncogene KRAS in autophagy regulation: Saccharomyces
cerevisiae as a model system. Presented by Sara Alves.

Azevedo, F, Chaves, SR, Johansson, B, Sousa, MJ and Côrte-Real, M. The role
of MAPK signaling pathways in acetic acid-induced cell death in Saccharomyces
cerevisiae. Presented by Flávio Azevedo.

Rego A., Costa A.M., Chaves S.R., Moradas-Ferreira P., Côrte-Real M. and Costa V. Role of ceramide pathway in apoptotic signalling induced by acetic acid in yeast. Presented by António Rego.

==============================

50th Annual Meeting of the American Society for Cell Biology, Philadelphia, USA, 11-15 December, 2010:

Chaves, SR, Rosenblum J Efficient Nuclear Transport of Structurally Disturbed Cargo: Mutations in a cargo protein switch its cognate karyopherin

==============================

XXXV Jornadas de Genética, Universidade do Minho, Campus de Gualtar, Braga, May31-June02 2010:

Rego A., Costa A.M., Chaves S.R., Moradas-Ferreira P., Côrte-Real M. and Costa V. Role of ceramide pathway in apoptotic signalling induced by acetic acid in yeast. Presented by António Rego.

==============================

I3S Scientific Retreat, Póvoa de Varzim, May 6-7, 2010:

Rego A., Costa A.M., Chaves S.R., Moradas-Ferreira P., Côrte-Real M. and Costa V. Role of ceramide pathway in apoptotic signalling induced by acetic acid in yeast. Presented by António Rego.

Ongoing:

Masters:

Carla Carvalho- Novel yeast biosensors for environmental monitoring, co-supervision with Dr. Bruno Castro

Jorge Macedo- Toxicity assessment of new products and processes for the agri-food sector potentially implicated in human wellbeing, co-supervision with Dr. Ana Preto

Completed:

PhD:

António Rego, 2016: The role of ceramides in the mitochondrial apoptotic pathway induced by acetic acid FCT Ref SFRH/BD/79523/2011, co-supervision with Dr. Manuela Côrte-Real and Dr. Vítor Costa

Helena Pereira, 2015: The role of the yeast ortholog of human Cathepsin D in the mitochondria-dependent vacuolar apoptotic pathway FCT Ref SFRH/BD/73139/2010, co-supervision with Dr. Manuela Côrte-Real

Diana Silva - The role of mitochondrial effectors of cell death in isc1 mutants, concluded in 2017, co-supervision with Dr. Manuela Côrte-Real

Nuno Machado- The mechanism underlying proteasome-mediated sensitivity to the chemotherapeutic agent cisplatin, concluded in 2017, co-supervision with Dr. Manuela Côrte-Real 

Filipa Mendes- Bax activation by PKCepsilon modulation,concluded in 2017, co-supervision with Dr. Sara Alves

Soraia Pereira:Bax activation by PKCepsilon modulation: An approach to eliminate cancer cells, concluded in 2015, co-supervision with Dr. Manuela Côrte-Real

Vanessa Campos: The role of mitochondrial effectors of cell death in isc1 mutants, concluded in 2015, co-supervision with Dr. Manuela Côrte-Real

Vera Martins: Pathways regulating cisplatin-induced cell death in S. cerevisiae, concluded in 2015, co-supervision with Dr. Maria João Sousa

Elisabete Fernandes: Understanding the crosstalk between the mitochondria and the vacuole during acetic acid-induced apoptosis, concluded in 2014, co-supervision with Dr. Manuela Côrte-Real

Rita Cunha: The mechanism mediating nuclear translocation of the apoptosis inducing factor AIF, concluded in 2013, co-supervision with Dr. Manuela Côrte-Real

Rita Pacheco: The role of Cks proteins in apoptosis, concluded in 2013

Ana Duarte: Genome-wide identification of the genes involved in the positive and negative regulation of acetic acid-induced cell death, concluded in 2013, co-supervision with Dr. Maria João Sousa

Dulce Cunha: Characterization of cisplatin-induced cell death in S. cerevisiae, concluded in 2012

Flávio Azevedo: The involvement of MAPK signaling pathways in acetic acid-induced cell death, concluded in 2011, co-supervision with Dr. Bjorn Johannson