CBMA

Yeast comprises a conserved core programmed cell death process that shares several cell death regulators with mammalian cells, which play major roles in the pathogenesis of human diseases. At the same time, it lacks cell death regulators that have evolved in higher eukaryotes, which allows elucidating the basic molecular pathways of programmed cell death without interference from multifaceted regulation that has evolved in higher eukaryotes, thus overcoming the problem of cellular specificity often observed in studies using mammalian systems. It also comprises a simpler system to study the individual functional and mechanistic properties of mammalian apoptotic regulators by heterologously expressing these proteins.

We have been extensively studying apoptotic mechanisms and signalling pathways, namely those involving mitochondria membrane permeabilization and the release of apoptogenic factors in yeast, as well as the crosstalk between the mitochondria and the vacuole. In collaboration with other CBMA colleagues from the mammalian field we have also begun studies with mammalian cell lines and corroborated several of our major findings obtained in the yeast model. More recently, and keeping a straight collaboration with researchers from national /international institutions, we have been extensively using yeast as an “in vivo” system for functional and molecular studies of individual mammalian apoptotic regulators.

Our global aim is to continue elucidating the core programmed cell death regulatory network in living organisms, and ultimately uncover attractive molecular targets for the development of novel therapeutics of Human pathologies associated with cell death dysfunctions. 

Main scientific interests and expertise are Biology, Microbiology, Cellular and Molecular Biology. As can be checked in my Google scholar profile

  (http://scholar.google.pt/citations?user=vHmzUMIAAAAJ&;hl=pt-PT) or the ISI Web of Science (link to my researcher ID: http://www.researcherid.com/rid/B-6328-2013; and http://orcid.org/0000-0002-1423-1331, Scopus Author ID 7004646723) I published 8 book chapters and 78 full articles (first one published in 1989) that accumulate at the present time 2210 citations (ISI) in 1320 documents, which corresponds to an h-index of 22. I was invited as a speaker to 23 lectures in national and international symposia. I have been a peer review of different journals indexed in ISI and Scopus. I have evaluated research projects submitted to funding agencies from United Kingdom, Italy, Belgium and Austria, and I was involved in the supervision/co-supervision of 12 PhD (+ 3 ongoing), 24 master students (+ 3 ongoing), and 2 pos-doc (+ 1 ongoing), and 10 research fellows (in the scope of granted projects). I was main opponent in 15 MSc Juries, 12 PhD Juries (3 in Universities outside Portugal) and 7 Habilitation Juries. I was appointed evaluator of 15 professors in the Department of Biology of UMinho. I have participated in 23 research projects (national and European). Since I finished my PhD in 1991 I was Principal Investigator in 8 projects with a global funding over 310,000 euros (last 3 years).

Positions (last 10 years)

- Director of the PhD program on Molecular and Environmental Biology, Departamento de Biologia, Universidade do Minho, 2011 – present

- Vice-director of the Molecular and Environmental Biology Centre (CBMA), Fundação para a Ciência e Tecnologia (FCT) research unit, 2013 – present

- Member of the Scientific Council (Conselho Científico) of the School of Sciences, 2012 – present (elected)

- Member of the School Council (Conselho de Escola) of the School of Sciences, 2012 – present (elected)

- Member of the Pedagogic Council School of Sciences (Conselho Pedagógico), of the School of Sciences, Universidade do Minho, 2012 - present (elected)

- Full Professor - Professora Catedrática, School of Sciences, University of Minho, 2009 – present

- Vice-director of the School of Sciences, Universidade do Minho, 2006-2009

- Director of the Biology Centre (CB), FCT research unit, Departamento de Biologia, Universidade do Minho, 2003-2008

Ludovico P, Côrte-Real M. Yeast on the corner of life and death decisions Mech Ageing Dev. 2017 Jan;161(Pt B):199-200. doi: 10.1016/j.mad.2017.02.003.

Ferro S, Azevedo-Silva J, Casal M, Côrte-Real M, Baltazar F, Preto A. Characterization of acetate transport in colorectal cancer cells and potential therapeutic implications Oncotarget. 2016 Sep 21. doi: 10.18632/oncotarget.12156.

Trindade D, Pereira C, Chaves SR, Manon S, Côrte-Real M, Sousa MJ (2016) VDAC regulates AAC-mediated apoptosis and cytochrome c release in yeast. Microbial Cell. Vol. 3, No. 10, pp. 369 - 379; DOI: 10.15698/mic2016.10.533

Pereira C, Guedes J, Gonçalves M, Loureiro L, Castro L, Gerós H, Rodrigues L, Côrte-Real M (2016) "Lactoferrin selectively triggers apoptosis in highly metastatic breast cancer cells through inhibition of plasmalemmal V-H+-ATPase" Oncotarget DOI: 10.18632/oncotarget.11394

Oliveira A, Sárria, Moreira P, Joana Fernandes J, Castro L, Lopes I, Côrte Real M, Cavaco Paulo A, Maria Elisabete Cunha Dias Real Oliveira E, Castro Gomes A (2016) Effect of counter ions and combination of constituents on the toxicity of DODAX: MO nanocarriers in vitro and in vivo Toxicology Research Toxicol. Res., 2016,5, 1244-1255.

69. Simonyan L, Renault TT, da Costa Novais MJ, Sousa MJ, Côrte-Real M, Camougrand N, Gonzalez C, Manon S (2016) Regulation of Bax/mitochondria interaction by AKT.FEBS Lett. 2016 Jan;590(1):13-21. doi: 10.1002/1873-3468.12030. Epub 2015 Dec 30.

68. Acosta-Zaldívar M, Andrés MT, Rego A, Pereira CS, Fierro JF, Côrte-Real M. (2016) Human lactoferrin triggers a mitochondrial- and caspase-dependent regulated cell death in Saccharomyces cerevisiae. Apoptosis. 2016 Feb;21(2):163-73. doi: 10.1007/s10495-015-1199-9

Alves S, Castro L, Fernandes MS, Francisco R, Castro P, Priault M, Rodrigues Chaves S, Moyer MP, Oliveira C, Seruca R, Côrte-Real M, Sousa MJ, Preto (2015) A Colorectal cancer-related mutant KRAS alleles function as positive regulators of autophagy Oncotarget 13;6(31):30787-802. doi: 10.18632/oncotarget.5021

Pereira H, Oliveira C S F, Castro L., Preto A, Chaves S R, Côrte-Real M (2015) Yeast as a tool to explore cathepsin D function. Microbial Cell Vol. 1, No. 7, 225 - 234.

Oliveira CS, Pereira H, Alves S, Castro L, Baltazar F, Chaves SR, Preto A, Côrte-Real M (2015) Cathepsin D protects colorectal cancer cells from acetate-induced apoptosis through autophagy-independent degradation of damaged mitochondria. Cell Death Dis. 2015 Jun 18;6.

Vilela A, Amaral C, Schuller D, Mendes-Faia A, Côrte-Real M (2015) Combined use of Wallerstein and Zygosaccharomyces bailii modified differential media to isolate yeasts for the controlled reduction of volatile acidity of grape musts and wines. Journal of Biotech Research 6:43-53.

Pires E, Teixeira JA, Brányik T, Côrte-Real M, Vicente AA (2014) Maintaining yeast viability in continuous primary beer fermentation. Journal of the Institute of Brewing, 120(1): 52-59.

Rego A, Duarte A M, Azevedo F, Sousa, M J, Côrte-Real M and S R Chaves (2014) Cell wall dynamics modulate acetic acid-induced apoptotic cell death of Saccharomyces cerevisiae Microbial Cell, Vol. 1, No. 9, pp. 303 -314. 

Pires E, Teixeira J, Branyik, T, Côrte-Real M, Brandão T, Vicente A (2014) High-gravity primary continuous beer fermentation by flocculated biomass". J Inst Brew. 120:486-494. 

Côrte-Real, M. and Madeo, F. (2013). Yeast programed cell death and aging: Frontiers in Oncology Nov 18;3:283. doi: 10.3389/fonc.2013.00283. eCollection 2013.

Teixeira V, Medeiros TC, Vilaça R, Pereira AT, Chaves SR, Côrte-Real M, Moradas-Ferreira P, Costa V.Rego A, Trindade D, Chaves S, Manon S, Costa V, Sousa MJ, Côrte-Real M (2013) The yeast model system as a tool towards the understanding of apoptosis regulation by sphingolipids, FEMS Yeast Research in production DOI: 10.1111/1567-1364.12096.

Pereira H., Azevedo F., Rego A., Sousa M.J., Chaves, S.R. and Côrte-Real M. (2013) The protective role of yeast cathepsin D in acetic acid-induced apoptosis depends on ANT (Aac2p) but not on the voltage-dependent channel (Por1p) FEBS Letters 587, 200–205.

Rodrigues J, Silva RD, Noronha H, Pedras A, Gerós H, Côrte-Real M (2013) Flow cytometry as a novel tool for structural and functional characterization of isolated yeast vacuoles. Microbiology 159 (Pt 5):848-56.

Pacheco A, Azevedo F, Rego A, Santos J, Chaves SR, Côrte-Real M and Sousa MJ C2-phytoceramide perturbs lipid rafts and cell integrity in Saccharomyces cerevisiae in a sterol-dependent manner (2013) PLoS ONE in production (IF 2012: 3.73).

Sousa M, Duarte AM, Fernandes TR, Chaves SR, Pacheco A, Leão C, Côrte-Real M, Sousa MJ. Genome-wide identification of genes involved in the positive and negative regulation of acetic acid-induced programmed cell death in Saccharomyces cerevisiae. BMC Genomics. 2013 Nov 28;14:838. doi: 10.1186/1471-2164-14-838

Marques C, Oliveira C.S. F., Alves S., Chaves S.R., Coutinho OP, Côrte-Real M and Preto A. Acetate-induced apoptosis in colorectal carcinoma cells involves lysosomal membrane permeabilization and cathepsin D release. Cell Death Dis. 2013 Feb 21;4.

Cunha D., Cunha R., Côrte-Real M. and Chaves S. R. (2013). Cisplatin-induced cell death in Saccharomyces cerevisiae is programmed and rescued by proteasome inhibition. DNA Repair (Amst). 2013 Jun 1;12(6):444-9.

Rego A, Costa M, Chaves SR, Matmati N, Pereira H, Sousa MJ, Moradas-Ferreira P, Hannun YA, Costa V, Côrte-Real M. (2012). Modulation of mitochondrial outer membrane permeabilization and apoptosis by ceramide metabolism. PLoS ONE, 7: e48571. DOI: 10.1371/journal.pone.0048571

Barbosa AD, Graça J, Mendes V, Chaves SR, Amorim MA, Mendes MV, Moradas-Ferreira P, Côrte-Real M, Costa V. (2012) “Activation of the Hog1p kinase in Isc1p-deficient yeast cells is associated with mitochondrial dysfunction, oxidative stress sensitivity and premature aging”. Mech Ageing Dev. 2012 May;133(5):317-30. doi: 10.1016/j.mad.2012.03.007. Epub 2012 Mar 16

Silva RD, Saraiva L, Coutinho I, Gonçalves J, Côrte-Real M (2012) Yeast as a powerful model system for the study of apoptosis regulation by protein kinase C isoforms. Curr Pharm Des. 18(17): 2492-500. Review Silva RD, Manon S, Gonçalves J, Saraiva L, Côrte-Real M. (2011). The importance of humanized yeast to better understand the role of bcl-2 family in apoptosis: finding of novel therapeutic opportunities. Current Pharmaceutical Design, 17: 246-55. DOI: 10.2174/138161211795049651

Sousa MJ, Azevedo F, Pedras A, Marques C, Coutinho OP, Preto A, Gerós H2, Chaves SR and Côrte-Real M. (2011) Vacuole-mitochondrial crosstalk during apoptosis in yeast: a model for understanding lysosomal mitochondrial mediated apoptosis in mammals. Biochemical Society Transactions,39(5):1533-7, (Invited minireview).

Silva RD, Manon S., Gonçalves J., Saraiva L., Côrte-Real M. (2011) The importance of humanized yeast to better understand the role of Bcl-2 family in apoptosis: finding of novel therapeutic opportunities. Curr Pharm Des. 17:246-55.

Silva RD, Manon S., Gonçalves J., Saraiva L., Côrte-Real M. (2011) Modulation of Bax mitochondrial insertion and induced cell death in yeast by mammalian protein kinase Calpha. Exp Cell Res. 1:781-90.

Pereira, C., Chaves, S., Alves, S., Salin, B., Camougrand, N., Manon, S., Sousa, M.J. and Côrte-Real, M. (2010) Mitochondrial degradation in acetic acid-induced yeast apoptosis: the role of Pep4 and the ADP/ATP carrier. Mol Microbiol 76(6), 1398-410.

Pereira, C., Silva, R.D., Saraiva, L., Johansson, B., Sousa, M.J. and Côrte- Real, M. (2008) Mitochondria dependent apoptosis in yeast. Biochim Biophys Acta. 2008 Jul;1783(7):1286-302. doi: 10.1016/j.bbamcr.2008.03.010. Epub 2008 Mar 25. Review.

Pereira C, Camougrand N, Manon S, Sousa MJ, Côrte-Real M. (2007) ADP/ATP carrier is required for mitochondrial outer membrane permeabilization and cytochrome c release in yeast apoptosis. Mol Microbiol 66, 571-82. Commented at Molecular Microbiology, Micro Commentary by Lawen A. Another piece of the puzzle of apoptotic cytochrome c release, 66(3):553-6.

Saraiva L, Silva RD, Pereira G, Gonçalves J e Côrte-Real M. (2006) “Specific modulation of apoptosis and Bcl-xL phosphorylation in yeast by distinct mammalian protein kinase C isoforms” Journal of Cell Science, 119: 3171-3181. 

 Ludovico, P., Rodrigues, F., Almeida, A., Silva, M. T, Barrientos, A. and Côrte-Real, M. (2002) "Cytochrome c release and mitochondria involvement in programmed cell death induced by acetic acid in Saccharomyces cerevisiae" Molecular Biology Cell, 13: 2598/2606.  

• EP - INTERREG V A España Portugal (POCTEP), CTB - CLUSTER TRANSFRONTERIZO DE BIOTECNOLOGIA. Consolidacion , crecimiento y competitividad del sector biotecnologico transfronterizo, team member.

• Identification of new kinases that phosphorylate the pro-apoptotic protein Bax, Acordos Bilaterais Programa PESSOA 2017-2019, project´s PI.

• EP - INTERREG V A España Portugal (POCTEP), CTB - CLUSTER TRANSFRONTERIZO DE BIOTECNOLOGIA. Consolidacion , crecimiento y competitividad del sector biotecnologico transfronterizo, team member

• Identification of new kinases that phosphorylate the pro-apoptotic protein Bax, Acordos Bilaterais Programa PESSOA 2017-2019, project´s PI.

• Phosphoregulation of Bax-dependent cell death- Phosphobax FCT-ANR/BEX-BCM/0175/2012, 2013-2016, project´s PI.

• Função da esfingomielinase neutra da levedura na função mitocondrial e homeostasia do ferro, FCT-PTDC/BBB-BQB/1850/2012, 2013-2015, task leader.

• Regulação da activação de Bax pelo oncogene PKCepsilon: Um método para eliminar células cancerígenas, FCT-EXPL/BIM-ONC/0056/2012, 2013-2014, member of the team.
( In the scope of this project, 1 paper was published and 2 manuscripts were prepared. A master thesis was completed and the results were presented in a national meeting). 

• ZygoSacAR-Mecanismos de resistência a ácidos fracos em leveduras contaminantes alimentares: do modelo experimental Saccharomyces cerevisiae a Zygosaccharomyces spp, FCT, 2010-2013. PTDC/AGR-ALI/102608/2008, member of the team.

• Elucidação da via apoptótica induzida pela ceramida: modulação de isoformas da Cínase C de Proteína, FCT, 2009-2012, project´s PI.
(In the scope of this project, we published 5 articles in international journals with peer review, 1 book chapter, 1 article is under review, and 4 manuscripts were prepared for publication; 1 PhD thesis and 7 Masters thesis have also been completed; several works were presented at national and international meetings with 10 and 7 communications, respectively, of which 5 as invited speakers).

• Role of ceramides and ceramides channels in the process of mitochondrial membrane permeabilization in the process of programmed cell death in yeast, Cooperação Portugal/França-Programa Pessoa, 2010-2011, project´s PI.

• Identification of Molecular components involved in the mitochondrial membrane permeabilization during programmed cell death: role of the ANT, VDAC and ceramides. Acções Universitárias Integradas Luso-Francesas / PAUILF 2010-2011, project´s PI.

• Investigating the role of Cofilin in mitochondrial permeabilisation and apoptosis, Acções Integradas Luso-Britânicas, CRUP, 2010, project´s PI.

• Molecular mechanismos of autophagic cell death in yeast, Acções integradas de Cooperação Científica e Técnica Luso-Francesas, Programa PESSOA 2007-2008, project´s PI.

• "Volatile phenol producing yeasts: a big concern in the wine industry that needs to be understood". The project was carried out from 2005 to 2008 and was financed by POCTI - Programa Operacional "Ciência, Tecnologia, Inovação", ref.ª POCTI/AGR/56771/2004, task leader.

• “Mechanisms of cell death induced by ethanol and acetic acid in Saccharomyces cerevisiae” The project was carried out from 2004 to 2005 and was financed by Acções integradas de Cooperação Científica e Técnica Luso-Francesas, Programa PESSOA 2004/2005, task leader.

• "Security and control of wine quality: implementation of rapid thecniques to microbial tractability and monitoring" The project was carried out from 2004 to 2006 and was financed by Acção 1 da Medida 8 do Programa AGRO, ref.ª 762 do INIAP, member of the team.

• "Involvement of sphyngolipids in aging and cell death of Saccharomyces cerevisiae – role of sphyngomielinase Isc 1 p". The project was carried out from 2003 to 2006 and was financed by Eixo 2, Medida 2.3 do POCTI – Programa Operacional "Ciência, Tecnologia, Inovação" do QCAIII, ref.ª POCTI/BCI/45066/2002, task leader.

• "Biose - Sutidies and Services in Biology". The project was carried out from 2002 to 2004 and was financed by Contrato Programa do Projecto nº 40/00874 "Empreender na Universidade", in the scope of Medida 2.2 A.2 do POE, project´s PI.

• "Removal of acetic acid from grape musts and sour wines". The project was carried out from 1999 to 2000 and was financed by PRAXIS, member of the team.

• "Utilization of Fungicides in Vitiviniculture: Effects in yeasts and wine fermentations". The project was carried out from 1997 to 1999 and was financed by PAMAF. Member of the team.

• "Isolation and characterization of yeasts: aplications in enology". project was carried out from 1996 to 1999 and was financed by PAMAF, member of the team.

• "Spoilage yeasts in food and beverages: characterization and ecology for improved diagnosis and culture". The project was carried out from 1993 to 1996 and was finaced by CEE through the program "Agriculture and Agro-Industry, Including Fisheries", member of the team.
• "Utilization of gluconeogenic substrates by non conventional yeasts. Biochemical mechanisms". The project was carried out from 1992 to 1995 and was financed by JNICT, project´s PI.

• "Transport of organic acids and its regulationin yeasts". The project was carried out from 1992 to 1995 and was financed by Glaxo Farmacêutica, member of the team.

• "Utilization of organc acids by industrial yeasts: fundamental and applied issues". The project was carried out on 1993 and was financed by JNICT, member of the team.

• "Transport and utilization of malic acid and other dicarboxylic acids by enological yeasts". Member of the research team.The project was carried out from 1987 to 1991 e foi financiado pela JNICT, member of the team.

59. Yeast programed cell death and aging. Côrte-Real M, Madeo F. Front Oncol. 2013 Nov 18;3:283. doi: 10.3389/fonc.2013.00283.

58 .Sousa M, Duarte AM, Fernandes TR, Chaves SR, Pacheco A, Leão C, Côrte-Real M, Sousa MJ. Genome-wide identification of genes involved in the positive and negative regulation of acetic acid-induced programmed cell death in Saccharomyces cerevisiae. BMC Genomics. 2013 Nov 28;14:838. doi: 10.1186/1471-2164-14-838

57. Rego A, Trindade D, Chaves S,Manon S, Costa V, Sousa MJ, Côrte-Real M (2013) The yeast model system as a tool towards the understanding of apoptosis regulation by sphingolipids, FEMS Yeast Research in production DOI: 10.1111/1567-1364.12096.

56. Pacheco A, Azevedo F, Rego A, Santos J, Chaves SR, Côrte-Real M and Sousa MJ C2-phytoceramide perturbs lipid rafts and cell integrity in Saccharomyces cerevisiae in a sterol-dependent manner (2013) PLoS ONE in production (IF 2012: 3.73).

55. Bessa C, Pereira C, Leão M, Maciel C, Gomes S, Gonçalves J, Corte-Real M, Costa V, Saraiva L (2013) Using yeast to uncover the regulation of protein kinase Cd by ceramide FEMS Yeast Research Aug 12. doi: 10.1111/1567-1364.12069. [Epub ahead of print].

54. Rodrigues J, Silva RD, Noronha H, Pedras A, Gerós H, Côrte-Real M (2013) Flow cytometry as a novel tool for structural and functional characterization of isolated yeast vacuoles. Microbiology 159 (Pt 5):848-56.

53. Cunha D., Cunha R.,Côrte-Real M. and Chaves S. R. (2013). Cisplatin-induced cell death in Saccharomyces cerevisiae is programmed and rescued by proteasome inhibition. DNA Repair (Amst). 2013 Jun 1;12(6):444-9.

52. Marques C:, Oliveira C.S. F., Alves S., Chaves S.R., Coutinho OP, Côrte-Real M and Preto A. Acetate-induced apoptosis in colorectal carcinoma cells involves lysosomal membrane permeabilization and cathepsin D release. Cell Death Dis. 2013 Feb 21;4.

51. Vilela A, Schuller D, Mendes-Faia A and Côrte-Real M. Reduction of volatile acidity of acidic wines by immobilized Saccharomyces cerevisiae cells (2013). Appl Microbiol Biotechnol doi 10.1007/s00253-013-4719-y

50. Pereira H., Azevedo F., Rego A., Sousa M.J., Chaves, S.R. and Côrte-Real M. (2013) The protective role of yeast cathepsin D in acetic acid-induced apoptosis depends on ANT (Aac2p) but not on the voltage-dependent channel (Por1p) FEBS Letters 587, 200–205.

49. Rodrigues F, Sousa MJ, Ludovico P, Santos H, Côrte-Real M, Leão C. The fate of acetic acid during glucose co-metabolism by the spoilage yeast Zygosaccharomyces bailii (2012). PLoS ONE 7(12):e52402. doi: 10.1371/journal.pone.0052402. Epub 2012 Dec 28.

48. Rego A., Costa A.M., Chaves S.R., Pereira H.P., Sousa M.J., Moradas-Ferreira P., Costa V. and Côrte-Real M. (2012) Modulation of mitochondrial outer membrane permeabilization and apoptosis by ceramide metabolism. PLoS ONE 7(11):e48571. doi:10.1371/journal.pone.0048571.

47. Barbosa AD, Graça J, Mendes V, Chaves SR, Amorim MA, Mendes MV, Moradas-Ferreira P, Côrte-Real M, Costa V. (2012) “Activation of the Hog1p kinase in Isc1p-deficient yeast cells is associated with mitochondrial dysfunction, oxidative stress sensitivity and premature aging”. Mech Ageing Dev, 133 (2012) 317–330.

46. Silva RD, Saraiva L, Coutinho I, Gonçalves J, Côrte-Real M (2012) Yeast as a powerful model system for the study of apoptosis regulation by protein kinase C isoforms. Curr Pharm Des. 2012;18(17):2492-500.

45. Sousa MJ, Azevedo F, Pedras A, Marques C, Coutinho OP, Preto A, Gerós H2, Chaves SR and Côrte-Real M. (2011) Vacuole-mitochondrial crosstalk during apoptosis in yeast: a model for understanding lysosomal-mitochondrial mediated apoptosis in mammals. Biochemical Society Transactions,39(5):1533-7, (Invited mini-review).

44. Fontes N, Côrte-Real M, and Gerós H. (2011) New observations on the integrity, structure and physiology of flesh cells from fully ripened grape berry. Am. J. Enol. Vitic. 2011, doi: 10.5344 /ajev.2011. 10126.

43. Coutinho I, Pereira C, Pereira G, Gonçalves J, Côrte-Real M, Saraiva L. (2011) Distinct regulation of p53-mediated apoptosis by protein kinase Ca, d, e and z: Evidence in yeast for transcription-dependent and -independent p53 apoptotic mechanisms. Exp Cell Res. 317: 1147-58.

42. Silva RD, Manon S., Gonçalves J., Saraiva L., Côrte-Real M. (2011) The importance of humanized yeast to better understand the role of Bcl-2 family in apoptosis: finding of novel therapeutic opportunities. Curr Pharm Des. 17:246-55.

41. Silva RD, Manon S., Gonçalves J., Saraiva L., Côrte-Real M. (2011) Modulation of Bax mitochondrial insertion and induced cell death in yeast by mammalian protein kinase Calpha. Exp Cell Res. 1:781-90.

40. Vilela-Moura, A., Schuller, D., Mendes-Faia, A., Silva, R.D., Chaves, S.R., Sousa, M.J. and Côrte-Real, M. (2011) The impact of acetate metabolism on yeast fermentative performance and wine quality: reduction of volatile acidity of grape musts and wines Appl Microbiol and Biotechnol, 89:271-80.

39. Vilela-Moura, A., Schuller, D., Mendes-Faia, A., Côrte-Real, M. (2010) Effects of acetic acid, ethanol and SO2 on the removal of volatile acidity from acidic wines by two Saccharomyces cerevisiae commercial strains. Appl Microbiol Biotechnol 87:1317-26.

38. A. Vilela-Moura, D. Schuller, V. Falco, A. Mendes-Faia and M. Côrte-Real (2010) Effect of refermentation conditions and micro-oxygenation on the reduction of volatile acidity by commercial Saccharomyces cerevisiae strains and their impact on the aromatic profile of wines Int J Food Microbiol 141:165-72.

37. Pereira C, Chaves S, Alves S, Salin B, Camougrand N, Manon S, Sousa M J and Côrte-Real M (2010) Mitochondrial degradation in acetic acid-induced yeast apoptosis: the role of Pep4 and the ADP/ATP carrier. 76(6):1398-410.

36. Coutinho I, Pereira G, Leão M, Gonçalves J, Côrte-Real M,, Saraiva L. (2009). Differential regulation of p53 function by protein kinase C isoforms revealed by a yeast cell system. FEBS Letters 583 (2009) 3582–3588.

35. Coutinho I, Pereira G, Simões MF, Côrte-Real M, Gonçalves J, Saraiva L (2009) Selective activation of protein kinase C-delta and -epsilon by 6,11,12,14-tetrahydroxy-abieta-5,8,11,13-tetraene-7-one (coleon U).Biochem Pharmacol. 78:449-59.

34. Vilela-Moura A, Schuller D, Mendes-Faia A, Côrte-Real M (2008) Reduction of volatile acidity of wines by selected yeast strains. Appl Mic Biotechnol 80:881-890.

33. Pereira C, Silva RD, Saraiva L, Johansson B, Sousa MJ, Côrte-Real M (2008) Mitochondriadependent apoptosis in yeast. BBA - Molecular Cell Research, Special Issue: Apoptosis in Yeast,1783:1286-1302.

32. Almeida T, Marques M, Mojzita D, Amorim MA, Silva RD, Almeida B, Rodrigues P, Ludovico P,Hohmann S, Moradas-Ferreira P, Côrte-Real M, Costa V. (2008) Isc1p plays a key role inhydrogen peroxide resistance and chronological lifespan through modulation of iron levels andapoptosis. Mol Biol Cell. 19:865-876.

31. Pereira C, Camougrand N, Manon S, Sousa MJ, Côrte-Real M. (2007) ADP/ATP carrier is required for mitochondrial outer membrane permeabilization and cytochrome c release in yeast apoptosis. Mol Microbiol 66, 571-82. Commented at Molecular Microbiology, MicroCommentaryby Lawen A. Another piece of the puzzle of apoptotic cytochrome c release, 66(3):553-6.

30. Guaragnella N, Pereira C, Sousa MJ, Antonacci L, Passarella S, Côrte-Real M, Marra E, Giannattasio S. (2006) YCA1 participates in the acetic acid induced yeast programmed cell death also in a manner unrelated to its caspase-like activity. FEBS Lett. 580(30): 6880-4.

29. Gabriela R, Côrte-Real M and Björn J(2006) Characterization of DNA damage in yeast apoptosis induced by hydrogen peroxide, acetic acid, and hyperosmotic shock. Mol. Biol. Cell 17(10): 4584-4591. 2006.

28. Saraiva L, Silva Rui, Pereira G, Gonçalves J, Côrte-Real M (2006). Specific modulation of apoptosis and Bcl-xL phosphorylation in yeast by distinct mammalian protein kinase C isoforms. J. Cell Science 119, 3171-3181.

27. Giannattasio, S., Guaragnella, N., Côrte-Real, M., Passarella, S. & Marra, E. 2005 “Acid stress adaptation protects Saccharomyces cerevisiae from acetic acid-induced programmed cell death” Gene, 354: 93-98.

26. Côrte-Real, M. (2004) The Second International Meeting on Yeast Apoptosis (IMYA 2), Smolenice, Slovak Republic, 17–20 September 2003. FEMS Yeast Research, 4(8): 887-888.

25. Wissing, S., Ludovico, P., Herker, E., Buttner, S., Engelhardt, S.M., Decker, T., Link, A., Proksch, A., Rodrigues, F., Côrte-Real, M., Frohlich, K.U., Manns, J., Cande, C., Sigrist, S.J., Kroemer, G. & Madeo, F. (2004) An AIF orthologue regulates apoptosis in yeast. Journal of Cell Biology, 166(7): 969-974.

24. Rodrigues, F., Zeeman, A.-M., Cardoso, H., Sousa, M. J., Steensma, H. Y., Côrte-Real, M. and Leão, C. (2004) “Isolation of acetyl-CoA synthetase gene (ZbACS2) from Zygosaccharomyces bailii”. Yeast, 21(4): 325-331.

23. Ludovico, P., Sansonetty, F., Silva, M. T. and Côrte-Real, M. (2003) “Acetic acid induces a programmed cell death in the food spoilage yeast Zygosaccharomyces bailii” Yeast FEMS Research, 1522: 1-6

22. Veiga, A., Arrabaça, J., Sansonetty, F., Ludovico, P., Côrte-Real, M. and Loureiro-Dias, C. (2003) "Energy conversion coupled to cyanide-resistant respiration in the yeasts Pichia membranifaciens and Debaryomyces hansenii" Yeast FEMS Research, 1529: 1-8.

21. Rodrigues, F., Ludovico, P., Sousa, M. J., Steensma, Y., Côrte-Real, M. and Leão, C. (2002) "The spoilage yeast Zygosaccharomyces bailii forms mitotic spores: A screening method for haploidization" Appl Environ. Microbiol., 69: 649-653.

20. Ludovico, P., Rodrigues, F., Almeida, A., Silva, M. T, Barrientos, A. and Côrte-Real, M. (2002) "Cytochrome c release and mitochondria involvement in programmed cell death induced by acetic acid in Saccharomyces cerevisiae" Molecular Biology Cell, 13: 2598/2606.

19. Ludovico, P., Sansonetty, F. and Côrte-Real, M. (2001) “Assessment of mitochondrial membrane potential in yeast cell populations by flow cytometry” Microbiology, 147:3335-3343.

18. Ludovico, P., Sousa, M. J., Silva, M.T., Leão, C. and Côrte-Real, M. (2001). “Saccharomyces cerevisiae commits to a programmed cell death process in response to acetic acid” Microbiology 147:2409-2415. Commented at Microbiology Today from Society for General Microbiology, “Hot of the Press”, volume 28 November, 2001.

17. Merico, A., Rodrigues, F., Côrte-Real, M., Porro, D., Ranzi, B. M. and Compagno, C. (2001) "Isolation and sequence analysis of the gene enconding triose phosphate isomerase from Zygosaccharomyces bailii" YEAST 18(9):775-80.

16. Rodrigues, F., Hemert, M., Steensma, H. Y., Côrte-Real, M. and Leão, C. (2001) “Red fluorescent protein (DsRed) as a reporter in Saccharomyces cerevisiae” J. Bacteriol. 183, 3791-3794.

15. Rodrigues, F., Zeeman, A. M., Alves, C., Sousa, M. J., Steensma, H. Y., Côrte-Real, M. and Leão, C. (2001) "Construction of a genomic library of the food spoilage yeast Zygosaccharomyces bailii and isolation of the ß-isopropylmalate dehydrogenase gene (ZbLEU2)" FEMS Yeast Research 1, 67-71.

14. Rodrigues, F., Côrte-Real, M., Leão, C., van Dijken, J. P. and Pronk, J. T. (2001) "Oxygen requirements of the food spoilage yeast Zygosaccharomyces bailii in synthetic and complex media" Appl. Env. Microbiol. 67(5), 2123-2128.

13. Schuller, D., Côrte-Real, M. and Leão, C. (2000) "Evaluation of a differential/selective medium for the enumeration of the wine spoilage yeast Zygosaccharomyces bailii" J. Food Prot. 63 (11), 1570-1575.

12. Prudêncio, C., Sansonetty, F., Sousa, M. J., Côrte-Real, M. and Leão, C. (2000) “Rapid detection of efflux pumps and their relation with drug resistance in yeast cells” Cytometry 39, 26-35.

11. Fernandes, L., Côrte-Real, M. and Leão, C. (1999) "A peculiar behaviour for cell death induced by weak carboxylic acids in the wine spoilage yeast Zygosaccharomyces bailii" Letters in Appl. Microbiol. 28, 345-349.

10. Prudêncio, C., Sansonetty, F. and Côrte-Real, M. (1998) "Flow cytometric assessment of cell structural and functional changes induced by acetic acid in the yeasts Zygosaccharomyces bailii and Saccharomyces cerevisiae" Cytometry, 31, 1-7.

9. Sousa, M. J., Rodrigues, F., Côrte-Real, M. and Leão, C. (1998) "Mechanisms underlying the transport and intracellular metabolism of acetic acid in the presence of glucose by the yeast Zygosaccharomyces bailii” Microbiol. 144, 665-670.

8. Fernandes, L., Côrte-Real, M., Loureiro, V., Loureiro-Dias, M. C. and Leão, C. (1997) "Glucose respiration and fermentation in Zygosaccharomyces bailii and Saccharomyces cerevisiae express different sensitivity patterns to ethanol and acetic acid" Lett. Appl. Microbiol., 25, 249-253.

7. Amador, P., Borges, F. and Côrte-Real, M. (1996) “Biochemical characterization of a mutant of the yeast Pichia anomala derepressed for malic acid utilization in the presence of glucose” FEMS Microbiol. Letters, 141, 227-231.

6. Sousa, M. J., Miranda, L., Côrte-real, M. and Leão, C. (1996) “Transport of acetic acid in Zygosaccharomyces bailii: effects of ethanol and their implications on the resistance of the yeast to acid environments“ Appl. and Environ. Microbiol. 62, 3152-3157.

5. Cássio F., Côrte-Real, M. and Leão, C. (1993) “Quantitative analysis of proton movements associated to the uptake rate of weak carboxylic acids". The yeast Candida utilis as a biological model” Biochem. Biophys. Acta, 1153, 59-66.

4. Côrte-Real, M. and Leão C. (1992) “Deacidification of grape must with derrepressed mutants of the wine yeast Hansenula anomala” Appl. Microbiol. Biotechnol, 36: 663-666.

3. Côrte-Real, M., Leão, C. and van Uden, N. (1990) “Inverse diauxy in the yeast Hansenula anomala: mutants derepressed for malic acid utilization in the presence of glucose” Appl. Environ. Microbiol., 56: 3402-3404.

2. Côrte-Real, M. and Leão, C. (1990) “Transport of malic acid and other dicarboxylic acids in the yeast Hansenula anomala” Appl. Environ. Microbiol., 56: 1109-1113.

1. Côrte-Real, M., Leão, C. and van Uden, N. (1989) “Transport of L(-) malic acid and other dicarboxylic acids in the yeast Candida sphaerica” Appl. Microbiol. Biotechnol., 31: 551-553.

12. Almeida, T., Marques, M., Mojzita, D., Osorio, H., Rodrigues, P., Amorim, M. A., Ludovico, P., Hohmann, S., Moradas-Ferreira, P., Corte-Real, M., Costa, V (2006). “Role of Isc1p in oxidative stress resistance and ageing in yeast” CHEMISTRY AND PHYSICS OF LIPIDS Volume: 143 Issue: 1-2 Pages: 83-84 Published: SEP.
11. Silva R., Sotoca, R Ludovico P, Sansonetty F, Peinado J, Côrte-Real M. (2004) “Saccharomyces cerevisiae commits to programmed cell death in response to hyperosmotic stress caused by glucose” CYTOMETRY PART A 59A: 128-128 MAY.
10. Almeida T, Marques M, Amorim MA, Ludovico P, Moradas-Ferreira P, Côrte-Real M. and Costa VF (2003). “Sphingolipid signalling in stress resistance and chronological life-span in Saccharomyces cerevisiae: the role of Isc1p” YEAST 20: S196-S196 Suppl. 1 JUL.
9. Ludovico PC, Rodrigues FJ, Almeida AJ, Silva MT., Barrientos A, Leão C and Côrte-Real M. (2002) “Involvement of mitochondria in the programmed cell death process induced by acetic acid in Saccharomyces cerevisiae” CYTOMETRY: 39-39 Suppl. 11.
8. Ludovico, P., Sousa, M. J., Silva, M. T., Leão, C. and Côrte-Real, M. (2001). “Programmed cell death in Saccharomyces cerevisiae induced by acetic acid” Yeast 18 (S1), pp. S354.
7. Ludovico, P., Salvador, A., Sansonetty, F., Ferreira, J., Côrte-Real, M. and Leão, C. (2000) "FCM measurements of changes in mitochondrial membrane potential in the yeasts Zygosaccharomyces bailii and Saccharomyces cerevisiae induced by acetic acid using Rh123 and propidium iodide" Cytometry, supplement 10, 100.
6. Prudêncio, C., Sansonetty, F., Sousa, M. J., Côrte-Real, M. and Leão, C. (2000) "FCM functional assay of multiple resistance of the yeast Debaryomyces hansenii to fluconazole, 5-fluorocytosine and amphotericin B. Evaluation of its reversion" Cytometry, supplement 10, 104.
5. Prudêncio, C., Sansonetty, F., Sousa, M. J., Côrte-Real, M. and Leão C. (1999) “Development of epifluorescense and flow cytometric methods for the detection of active efflux pumps in yeast” Folia Microbiologica 44:219.
4. Prudêncio, C., Sansonetty, F., Sousa, M. J. and Côrte-Real, M. (1998) “Evaluation of the specificity of yeast multidrug pumps for penconazol, benomyl and cycloheximide by flow cytometry” Cytometry, supplement 9:153.
3. Prudêncio, C., Sansonetty, F., Côrte-Real, M. and Leão, C. (1998) “Screening of multidrug pumps in yeasts” Cytometry, supplement 9:153.
2. Amador, P., Borges, F., Côrte-Real, M. and Leão, C. (1994) “Regulation of glucose and malic acid consumption by a derepressed mutant of the yeast Pichia anomala” Folia Microbiologica, 39/6:506.
1. Miranda, L., Sousa, M. J., Côrte-Real, M. and Leão, C. (1994) “Transport of acetic acid and other weak organic acids in the yeast Zygosaccharomyces bailii” Folia Microbiologica, 39/6:515.

8.  Côrte-Real M. and Madeo F. (2014). “Yeast programmed cell death and aging”. In: Frontiers Research Topic Ebook: Frontiers in Oncology, Frontiers Media SA. ISBN: 978-2-88919-232-8

7. Sousa, M. J., Ludovico, P., Rodrigues, F., Leão, C. and M. Côrte-Real M. "Stress and Cell Death in Yeast Induced by Acetic Acid" in Cell Metabolism - Cell Homeostasis and Stress Response, ISBN 978-953-307-978-3, edited by Paula Bubulya, 2012.

6. Sousa, M.J., Rodrigues, F., Côrte-Real, M. e Leão C. "Transporte transmembranar " In: Biologia Celular e Molecular, Carlos Azevedo e Claudio Sunkel (Eds), 5ª Edição, Lidel Edições Técnicas, Lisboa. In press.

5. Johansson, B., Saraiva, L. e Côrte-Real, M. "Nutrição e Crescimento Microbiano" In: Microbiologia, Wanda Ferreira, João C. Sousa e Nelson Lima (Eds), Lidel Edições Técnicas, Lisboa, 2010.

4. Schuller, D., Côrte-Real, M. and Leão, C. "Use of a differential/ culture medium for the enumeration of Zygosaccharomyces bailii, Saccharomyces cerevisiae and Pichia membranefaciens" in Non-conventional Yeasts in Genetics, Biochemistry, and Biotechnology Practical Protocols. Published by Springer Lab Manual, Chapter 67, 459 462, Wolf, Breunigand Barth Eds.

3. Rodrigues, F., Sousa, M. J., Côrte-Real, M. and Leão, C. "Zygosaccharomyces bailii: an yeast with a peculiar pattern for the regulation of acetic acid metabolism in the presence of glucose" In Non-conventional Yeasts in Genetics, Biochemistry, and Biotechnology Practical Protocols. Published by Springer Lab Manual, Chapter 64, 403 410, Wolf, Breunigand Barth Eds.

2. Côrte-Real, M., Ludovico, P., Rodrigues, F. e Leão, C. "Metabolismo Primário" In: Biotecnologia: Fundamentos e Aplicações, Nelson Lima e Manuel Mota (Eds), Lidel Edições Técnicas, Lisboa, 2003.

1. Fortuna, M., Sousa, M. J., Salvador, A., Sansonetty, F., Côrte-Real, M. and Leão, C. (2000) “Cell cycle analysis of Yeast. Current Protocols in Cytometry” (11.13.1-11.13.9), published by John Wiley & Sons, Inc.

International
• Leão, C., Côrte-Real, M., Schuller, D. “Culture medium for detection of Zygosaccharomyces”. Patente International nº PCT/PT00/00004.

National
• Schuller, D., Côrte-Real, M. and Leão, C. (1999) "Meio de cultura para a detecção das leveduras Zygosaccharomyces bailii e Zygosaccharomyces bisporus" Instituto Nacional da Propriedade Intelectual (INPI), patent 12480V.

3. Côrte-Real, M., Sansonetty, F., Ludovico, P., Prudêncio, C., Rodrigues, F., Fortuna, M., Sousa, M. J., Silva, M.T. e Leão, C. (2002) "Contributos da citologia analítica para estudos de biologia de leveduras" Boletim de Biotecnologia, 71, 19-33.

2. Prudêncio, C., Sansonetty, F. e Côrte-Real, M. (2000) "Avaliação por citometria de fluxo.de alterações estruturais e funcionais induzidas pelo ácido acético em leveduras", artigo 1, Boletim da Sociedade Portuguesa de Microbiologia.

1. Leão, C., Rodrigues, F., Paiva, S., Queirós, O., Schuller, D., Cássio, F., Cardoso, H., Sousa, M. J., Casal, M. e Côrte-Real, M. (2000) "Remoção zimológica da acidez volátil e fixa de mostos e vinhos: da fisiologia à manipulação genética de leveduras" Boletim de Biotecnologia, 66, 7-13.

Comunicações orais por convite

• 10th International Meeting on Yeast Apoptosis, 14th-18th May, 2014, Gotenberg, Sweeden
Oliveira C.S.F , Pereira H, Alves S, Chaves S R, Sousa M J, Preto A, Côrte-Real M. The yeast vacuolar protease Pep4p unveils a novel function of the human Cathepsin D during acetate-induced mitochondrial apoptosis in colorectal carcinoma cells

• 9th International Meeting on Yeast Apoptosis, 16th-20th September, 2012, Rome, Italy
Rego A, Costa AM, Chaves SR, Pereira HP, Sousa MJ, Moradas-Ferreira P, Costa V. and Côrte-Real M. Modulation of mitochondrial outer membrane permeabilization and apoptosis by ceramide metabolism.

• 8th International Meeting on Yeast Apoptosis, 2-6 May, 2011, University of Kent, Canterbury, England
. Chaves S, Pereira C, Marques C, Rodrigues A, Salin B, Alves S, Silva R, Gerós H, Coutinho OP, Preto A, Camougrand N, Manon S, Sousa MJ and Côrte-Real M. Vacuole-mitochondrial crosstalk during apoptosis induced by acetic acid in yeast.

• 14th Portugaliae Genetica, 17-18 Marsh, 2011, IPATIMUP,Porto
. Chaves S, Pereira C, Marques C, Rodrigues A, Salin B, Alves S, Silva R, Gerós H, Coutinho O, Preto A, Camougrand N, Manon S, Sousa M J and Côrte-Real M. What yeast can tell us about how cells commit suicide?

• Society of General Microbiology-Autumn 2010 Meeting University of Nottingham, Jubilee Campus, Nottingham 2010
. Chaves S, Pereira C, Alves S, Salin B, Camougrand N, Manon S, Sousa MJ and Côrte-Real M
Acetic acid-induced apoptosis in Saccharomyces cerevisiae

• 9th International Mycology Congress, Edinburgh 2010
. Chaves S, Pereira C, Alves S, Salin B, Camougrand N, Manon S, Sousa MJ and Côrte-Real M
A non-autophagic role for the vacuole in mitochondrial degradation during acetic acid-induced yeast apoptosis

• 1as Jornadas de Bioquímica, Universidade do Minho, Braga 2010
. Côrte-Real M e Sousa MJ. "Estudos de Morte Celular Programada na levedura Saccharomyces cerevisiae".

• 7th INTERNATIONAL MEETING ON YEAST APOPTOSIS, Graz, Austria, 2009
. Silva, R D, Manon, S, Gonçalves, J, Saraiva, L and Côrte-Real, M. "Modulation of Bax by Mammalian Protein Kinase C-alfa in Yeast".

• 12th INTERNATIONAL CONGRESS OF YEAST, Kiev, Ukraine. 2008
. Pereira, C, Camougrand, N, Manon, S, Sousa, M J and Côrte-Real “Communication between vacuole and mitochondria regulates mitochondrial degradation in yeast cells committed to apoptosis”.

• 6th INTERNATIONAL MEETING ON YEAST APOPTOSIS. Leuven, Belgium. 2008
. Pereira, C, Camougrand, N, Manon, S, Sousa, M J and Côrte-Real M “Mitochondrial fragmentation/degradation in Saccharomyces cerevisiae programmed cell death".

• 16AS JORNADAS DE BIOLOGIA PROFESSOR NICOLAU VAN UDEN, VIMEIRO, 2008
. Côrte-Real M, Pereira C, Silva RD, Ribeiro G, Sotoca R, Saraiva L, Pereira, G, Coutinho I, Cavaleiro E, Johanson B, Ludovico P, Rodrigues F, Almeida A, Barrientos, Wissing S, Herker E, Büttner S, Engelhardt SM, Decker T, Link A, Proksch A, Fröhlich KW Manns J, Candé, C, Sigrist, SJ, Kroemer G, Madeo F, Sansonetty F, Peinado JM, Gonçalves J, Sousa MJ, Camougrand N, Manon S, Silva M T, Leão C. "Contributions of yeast biology to the understanding of cell death regulation".

• 5th INTERNATIONAL MEETING ON YEAST APOPTOSIS. Kutna Hora, Check Republic. 2006
. Côrte-Real, M., Pereira, C., Camougrand, N., Manon, S., Ribeiro, G., Saraiva, L., Silva, R.D., Pereira, G., Gonçalves, J., Johansson, B. & Sousa, M.J. "Integrating mammalian protein expression and yeast apoptosis towards understanding cell death regulation".

• MICRO´2005 BIOTEC´ 2005, Póvoa de Varzim, 2005
. Côrte-Real M. “Yeast as a model to understanding apoptosis”.

• 4th INTERNATIONAL MEETING ON YEAST APOPTOSIS, Miami, USA, 2005
. Saraiva L., Silva R., Pereira G., Gonçalves J. Côrte-Real M. “Effects of mammalian PKC-alpha, -delta, -epsilon and –zeta in Saccharomyces cerevisiae acetic acid-induced apoptosis: an approach for the study of apoptosis regulation by individual PKC isoforms”.

• XX CONGRESSO NACIONAL DE MICROBIOLOGIA, Cáceres, Espanha, 2005
. Côrte-Real, M., Pereira, C., Silva, R., Sotoca, R., Saraiva, L., Johansson, B., Ludovico, P., Sansonetty, F., Gonçalves, J., Sousa, M.J., Manon, S., Silva, M.T., Peinado, J. M. “Cell death with apoptotic phenotype in yeasts”.

• 9º CONGRESSO DA SOCIEDADE IBÉRICA DE CITOMETRIA, Porto, 2005
. Côrte-Real, M. and Sansonetty, F. “Assessment of cell viability/activity of yeast populations by flow cytometry”.

• 3TH INTERNATIONAL MEETING ON YEAST APOPTOSIS, Salzburg, Austria, 2004
. Saraiva L., Silva R., Figueira V., Gonçalves J. Côrte-Real M. “Regulation of Saccharomyces cerevisiae programmed cell death by mammalian protein kinase C (PKC) isoforms”.

• 2ND INTERNATIONAL MEETING ON YEAST APOPTOSIS, Bratislava, ESLOVÁQUIA, 2003
. Sotoca, R., Silva, R., Ludovico, P., Sansonetty, F., Martinez-Peinado, J. and Côrte-Real, M. "Hyperosmotic Stress Caused by Glucose Induces Programmed Cell Death in Saccharomyces cerevisiae".

• VIII CONGRESO DE LA SOCIEDAD IBÉRICA DE CITOMETRIA, Madrid, ESPANHA, 2003
. Côrte-Real, M. and Sansonetty, F. "Phisiological Studies of Yeast Populations by Flow Cytometry”.

• XIII CONGRESSO NACIONAL DE BIOQUÍMICA, Lisboa, 2002
. Ludovico, P. C., Rodrigues, F. J., Almeida, A. J., Sousa, M. J., Silva, M. T., Barrientos, A., Leão, C. e Côrte-Real, M. "Programmed Cell Death in Yeast".

• XXI CONGRESS OF THE INTERNATIONAL SOCIETY FOR ANALYTICAL CYTOLOGY, San Diego, USA, 2002
. Rodrigues, F. J., Van Hemert, M., Steensma, H. Y. Côrte-Real, M. and Leão, C. "Red fluorescent protein (DsRed) as an in vivo marker in Saccharomyces cerevisiae".

. Ludovico, P. C., Rodrigues, F. J., Almeida, A. J., Silva, M. T., Barrientos, A., Leão C. and Côrte-Real, M. "Involvement of mitochondria in programmed cell death process induced by acetic acid in Saccharomyces cerevisiae".

• XXTH INTERNATIONAL CONFERENCE ON YEAST GENETICS AND MOLECULAR BIOLOGY, Prague, CHECOSLOVÁQUIA, 2001
. Ludovico, P., Sousa, M. J., Silva, M.T., Leão, C. and Côrte-Real, M. “Programmed cell death in Saccharomyces cerevisiae induced by acetic acid”.

• INTERNATIONAL SPECIALIZED SYMPOSIUM ON YEASTS, Lviv, UCRÂNIA, 2001
. Rodrigues, F., Ludovico, P., Van Hemert, M., Sousa, M. J., Cardoso, H., Sansonetty, F., Steensma, Y., Silva, M. T., Côrte-Real, M. and Leão, C. “Yeast responses to acetic acid stress”.

Pos-doc

Concluded

Johansson B. "Cytochrome c protein interactions involved in Saccharomyces cerevisiae programmed cell death", co-supervision, Departamento de Biologia da Universidade do Minho.

Pacheco A. "Elucidation of signaling pathways involved in ceramide-induced cell death", co-supervision, Departamento de Biologia da Universidade do Minho.

Ongoing

Chaves S. "Yeast as a model system to study mechanisms regulating cisplatin sensitivity and resistance".
Started post-doc fellowship in July 2013.

PhD Thesis:

Concluded

• Miranda L.  "Mecanismos subjacentes à resistência da levedura Zygosacharomyces bailii a ambientes extremos", co-supervision, 1998, Departamento de Biologia da Universidade do Minho.

• Prudêncio C. “Estudos conducentes à elucidação de mecanismos subjacentes à resistência pleiotrópica a drogas em leveduras”, 2000, Departamento de Biologia da Universidade do Minho.

• Rodrigues F.  "Regulação do metabolismo do ácido acético em Zygosaccharomyces bailii: estudos fisiológicos e moleculares", 2001, Departamento de Biologia da Universidade do Minho.

• Ludovico P.  "Alterações estruturais e funcionais da mitocôndria e sua relação com morte celular em Saccharomyces cerevisiae", 2002, Departamento de Biologia da Universidade do Minho.

• Pereira C. "Envolvimento de proteínas mitocondriais na apoptose de leveduras", co-supervision, 2008, Departamento de Biologia da Universidade do Minho.

• Almeida T.  "Envolvimento da Sinalização por esfingolípidos no envelhecimento e morte celular em Saccharomyces cerevisiae – função da esfingomielinase Isc 1 p", co-supervision, 2008, Instituto de Biologia Molecular e Celular, Universidade do Porto (IBMC/Universidade do Porto).

• Moura, A.  "Isolation and Characterization of Yeasts: Application in Controlled processes of Volatile Acidity Bio-reduction in Wines", co-supervision, 2010, Universidade de Trás-os Montes e Alto Douro.


• Silva, R. "A new approach for the study of individual PKC isoform modulation of Bcl-2/Bax mediated cell survival: searching for apoptosis based-therapeutic agents”, co-supervision, 2011, Departamento de Biologia, Universidade do Minho.

• Coutinho, I. "Study of p53-signalling pathway and pharmacological screening of small-molecules targeting p53 function", co-supervision, Faculdade de Farmácia, Universidade do Porto.

• Dário Trindade , PhD thesis on " Contribution of the adenine nucleotide carrier, porin, and sphingolipid metabolism to mitochondria membrane permeabilization in Saccharomyces cerevisiae", Departamento de Biologia, Universidade do Minho, 2013.

• Sara Alves, PhD thesis on " Impact of mutated KRAS signalling on autophagy regulation on colorectal carcinoma", Departamento de Biologia, Universidade do Minho, 2014.

Ongoing

• Ribeiro, G. " Analysis and screening for novel apoptotic proteases and nucleases", co-supervision, Departamento de Biologia, Universidade do Minho.

• Pereira, H. "The role of Pep4p, the vacuolar yeast protease ortholog of human Cathepsin D in mitochondria-dependent apoptosis",co-supervision, Departamento de Biologia, Universidade do Minho.

• Ferro, S. “Unravel the mechanisms of acetate induced apoptosis in colorectal carcinoma", co-supervision, Departamento de Biologia, Universidade do Minho.

• Castro, L. "Identification of protein kinases involved in the phosphoregulation of Bax-dependent cell death", co-supervision, Departamento de Biologia, Universidade do Minho.

Master Thesis:

Concluded

• Borges, F . Master Thesis in Ensino de Biologia e Geologia, entitled "Utilização de substratos glicolíticos e gluconeogénicos na levedura Hansenula anomala. Uma abordagem teórico-experimental para o ensino da biologia". Evaluation was performed in 1994, being the student aproved with the final classification of Very Good.

• Amador, P. Master Thesis in Genética Molecular Microbiana, entitled “Mecanismos subjacentes à utilização sequencial do ácido málico e da glucose por um mutante desreprimido da levedura Pichia anomala”. Evaluation was performed in 1995, being the student aproved with the final classification of Very Good.

• Pereira, C. Master Thesis in Genética Molecular Microbiana, entitled “Estudos sobre alterações funcionais induzidas pelo ácido acético em Saccharomyces cerevisiae e e Zygosaccharomyces bailii. Avaliação por citometria de fluxo”. Evaluation was performed in 1995, being the student aproved with the final classification of Very Good.

• Rodrigues, F. Master Thesis in Genética Molecular Microbiana, entitled "Estudos sobre a regulação do metabolismo intracelular de ácido acético na levedura Zygosaccharomyces bailii". Evaluation was performed in 1998, being the student aproved with the final classification of Very Good.

• Ludovico, P. Master Thesis in Genética Molecular Microbiana, entitled "Efeitos do ácido acético no potencial de membrana mitocondrial e sua relação com a perda de integridade e viabilidade celular em Zygosaccharomyces bailii e Saccharomyces cerevisiae. Estudos por citometria de fluxo e espectrofluorimetria". Evaluation was performed in 1999, being the student aproved with the final classification of Very Good.

• Fortuna, M. Master Thesis in Genética Molecular Microbiana, entitled "Estudos sobre os efeitos de fungicidas de utilização na vitivinicultura no crescimento e ciclo celular da levedura Saccharomyces cerevisiae"co-supervision. Evaluation was performed in 1999, being the student aproved with the final classification of Very Good.

• Schuller, D. Master Thesis in Mestrado em Ciências do Ambiente, entitled "Desenvolvimento de um meio de cultura selectivo/diferencial para a levedura de contaminação alimentar Zygosaccharomyces bailii", co-supervision. Evaluation was performed in 1998, being the student aproved with the final classification of Very Good.

• Silva, R. Master Thesis in Genética Molecular, entitled "Elucidação dos mecanismos de morte celular induzidos por choque hiperosmótico em Saccharomyces cerevisiae". Evaluation was performed in 2005, being the student aproved with the final classification of Very Good.

• Ribeiro, G. Master Thesis in Genética Molecular, entitled "Search for markers in ethanol induced cell death in Saccharomyces cerevisiae", co-supervision. Evaluation was performed in 2005, being the student aproved with the final classification of Very Good.

• Trindade, D. Master Thesis in Genética Molecular, entitled "Papel do Fps1p na resistência de Saccharomyces cerevisiae ao etanol e na resistência cruzada com o ácido acético”. Evaluation was performed in 2009, being the student aproved with the final classification of 18.

• Dóris, A. Master Thesis in Genética Molecular, entitled "Functional characterization of purified vacuoles and evaluation of their role in yeast apoptosis induced by acetic acid". Evaluation was performed in 2011, being the student aproved with the final classification of 18. 

• Afonso, A. Master Thesis in Genética Molecular, entitled "“Resistence of Saccharomyces cerevisiae to ethanol and protective effect of acetic acid”, co-supervision. Evaluation was performed in 2011, being the student aproved with the final classification of 18. 

• Rego, A. Master Thesis in Genética Molecular, entitled “ Characterization of the role of sphingolipid metabolism in acetic acid induced-apoptosis", co-supervision. Evaluation was performed in 2011, being the student aproved with the final classification of 19. PhD fellowship.

• Cunha, R. Master Thesis in Genética Molecular, entitled "The mechanism mediating nuclear translocation of the apoptosis inducing factor (AIF)”, co-supervision. Evaluation was performed in 2012, being the student aproved with the final classification of 19. Researcher fellowship.

• Sousa, M. Master Thesis in Genética Molecular entitled “Identification of genes involved in the regulation of acetic acid-induced programmed cell death ", co-supervision. Evaluation was performed in 2011, being the student aproved with the final classification of 19. Unknown job position.

• Fernandes, T. Master Thesis in Genética Molecular entitled "The role of phospholipids and of the actin-binding protein cofilin in Saccharomyces cerevisiae acetic acid-induced apoptosis ", co-supervision. Evaluation was performed in 2013, being the student aproved with the final classification of 19. Marie Curie fellowship.

• Barreto, J. Master Thesis in Sciences - teachers continuous training entitled " Current overview of the role of short-chain fatty acids in prevention or therapy of colorectal carcinoma", co-supervision. Evaluation was performed in 2013, being the student aproved with the final classification of 18. Secondary School teacher.

• Rocha, N. Master Thesis in Applied Biochemistry entitled "Role of apoptotic regulators in the protective effect of acetic acid against ethanol - induced cell death in Saccharomyces cerevisiae", co-supervision. Evaluation was performed in 2014, being the student aproved with the final classification of 18. Unknown job position.

Ongoing

•  Pereira, S.  "Bax activation by PKCepsilon modulation: An approach to eliminate cancer cells", co-supervision.

• Campos, V." The role of mitochondrial effectors of cell death in isc1 mutants", co-supervision.

• Fernandes, T. "Understanding the crosstalk between the mitochondria and the vacuole during acetic acid-induced apoptosis", co-supervision.

• Loureiro, L. " Functional characterization of mitochondrial suspensions by cytofluorimetric tehcniques towards the screening of mitochondrial target drugs", co-supervision.

• Gonçalves, M. "The role of the vacuole/lysosome in cell death", co-supervision.

•  Pereira, C. "Lactoferrin as a proton pump inhibitor targeting V-ATPase: a new mechanism of anticarcinogenic action", co-supervision.

• Silva, A. " Identifying yeast kinases and phosphatases involved in Bax-mediated cell death " , co-supervision.