CBMA

Centro de Biologia Molecular e Ambiental

Centre of Molecular and Environmental Biology

Raul Machado
Raul Machado
...
253605312
Assistant Professor/Researcher
Innovation for Life - Functional Genomics and Nanobiotechnology for Sustainable Living
Design, bioproduction, processing and characterization of genetically engineered protein-based materials

Current Research & Key Publications

  • Domain of Specialization

Molecular biotechnology with main focus on the design, bioproduction and purification of bioinspired protein-based polymers;
Processing of protein-based materials by electrospinning, solvent casting and self-assembly techniques;
Mechanical and physical characterization of biopolymers.

  • Present Investigation Interests

Genetically engineered protein-based materials;
Antimicrobial materials, surfaces and coatings;
Biopolymers for skin tissue engineering and drug delivery systems;
Nanosensors, biomimetics and nanobiotechnology.

 

da Costa A, Pereira AM, Gomes AC, Rodríguez-Cabello JC, Sencadas V, Casal M, Machado R. (2017). Single step fabrication of antimicrobial fibre mats from a bioengineered protein-based polymer. Biomedical Materials, 12(4), 045011. DOI: 10.1088/1748-605X/aa7104

Pereira AM, Machado R, da Costa A, Ribeiro A, Collins T, Gomes AC, Leonor IB, Kaplan DL, Reis RL, Casal M. (2017). Silk-based biomaterials functionalized with fibronectin type II promotes cell adhesion. Acta Biomaterialia, 47(1), 50-59. DOI: 10.1016/j.actbio.2016.10.002

Machado R, da Costa A, Sencadas V, Pereira AM, Collins T, Rodriguez-Cabello JC, Lanceros-Méndez S, Casal M (2015). Exploring the properties of genetically engineered Silk-Elastin-Like Protein films. Macromolecular Bioscience, 15: 1698-1709. DOI: 10.1002/mabi.201500132

 da Costa A, Machado R, Ribeiro A, Collins T, Thiagarajan V, Neves-Petersen MT, Rodríguez-Cabello JC, Gomes AC, Casal M (2015) Development of elastin-like recombinamer films with antimicrobial activity. Biomacromolecules, 16: 625-35. DOI: 10.1021/bm5016706

Machado R, da Costa A, Sencadas V, Garcia-Arévalo C, Costa C.M, Padrão J, Gomes A, Lanceros-Méndez S, Rodríguez-Cabello JC, Casal M (2013) Electrospun silk-elastin-like fibre mats for tissue engineering applications. Biomedical Materials, 8, 065009. DOI:10.1088/1748-6041/8/6/065009

Bessa P, Machado R, Nürnberger S, Dopler D, Banerjee A, Cunha AM, Rodríguez-Cabello C, Redl H, van Griensven M, Reis RL, Casal M (2010) Thermoresponsive self-assembled elastin-based nanoparticles for delivery of BMPs. Journal of Controlled Release, 142: 312–318. DOI: 10.1016/j.jconrel.2009.11.003

Araújo R, Silva C, Machado R, Casal M, Cunha AM, Rodriguez-Cabello C, Cavaco-Paulo A. (2009) Proteolytic enzyme engineering: a tool for wool. Biomacromolecules, 10: 1655-1661. DOI: 10.1002/term.245

Research Projects

EcoAgriFood: Innovative Green Products and Processes to Promote Agri-Food Bioeconomy. NORTE-01-0145-FEDER-000009 (2016-2019). Team Member.

FunBioPlas: Novel synthetic biocomposites for biomedical devices. ERA-IB-15-089 (2016-2019). Project Coordinator and Principal Investigator.

 

Publications

14. da Costa A, Pereira AM, Gomes AC, Rodríguez-Cabello JC, Sencadas V, Casal M, Machado R. (2017). Single step fabrication of antimicrobial fibre mats from a bioengineered protein-based polymer. Biomedical Materials, 12(4), 045011. DOI: 10.1088/1748-605X/aa7104

13. Pereira AM, Machado R, da Costa A, Ribeiro A, Collins T, Gomes AC, Leonor IB, Kaplan DL, Reis RL, Casal M. (2017). Silk-based biomaterials functionalized with fibronectin type II promotes cell adhesion. Acta Biomaterialia, 47(1), 50-59. DOI: 10.1016/j.actbio.2016.10.002

12. Barroca M, Rodrigues P, Sobral R, Costa MMR, Chaves SR, Machado R, Casal M, Collins T. (2016). Antibiotic free selection for the high level biosynthesis of a silk-elastin-like protein. Scientific Reports, 6, 39329. DOI: 10.1038/srep39329

11. Machado R, da Costa A, Sencadas V, Pereira AM, Collins T, Rodriguez-Cabello JC, Lanceros-Méndez S, Casal M. (2015). Exploring the properties of genetically engineered Silk-Elastin-Like Protein films. Macromolecular Bioscience, 15: 1698-1709. DOI: 10.1002/mabi.201500132

10. Padrão J, Machado R, Casal M, Lanceros-Méndez S, Rodrigues LR, Dourado F, Sencadas V. (2015). Antibacterial performance of bovine lactoferrin-fish gelatine electrospun membranes. International Journal of Biological Macromolecules, 81: 608-614. DOI: 10.1016/j.ijbiomac.2015.08.047

9. da Costa A, Machado R, Ribeiro A, Collins T, Thiagarajan V, Neves-Petersen MT, Rodríguez-Cabello JC, Gomes AC, Casal M. (2015). Development of elastin-like recombinamer films with antimicrobial activity. Biomacromolecules, 16: 625-35. DOI: 10.1021/bm5016706

8. Collins T, Barroca M, Branca F, Padrão J, Machado R, Casal M. (2014). High level biosynthesis of a silk-elastin-like protein in E. coli. Biomacromolecules, 14: 2701-8. DOI: 10.1021/bm5005564

7. Machado R, da Costa A, Sencadas V, Garcia-Arévalo C, Costa C.M, Padrão J, Gomes A, Lanceros-Méndez S, Rodríguez-Cabello JC, Casal M. (2013). Electrospun silk-elastin-like fibre mats for tissue engineering applications. Biomedical Materials, 8(6), 065009. DOI:10.1088/1748-6041/8/6/065009

6. Machado R, Azevedo-Silva J, Correia C, Collins T, Arias FJ, Rodríguez-Cabello JC, Casal M. (2013). High level expression and facile purification of recombinant silk-elastin-like polymers in auto induction shake flask cultures. AMB Express, 3(1), 1-15. DOI:10.1186/2191-0855-3-11

5. Collins T, Azevedo-Silva J, da Costa A, Branca F, Machado R, Casal M. (2013). Batch production of a silk-elastin-like protein in E. coli BL21(DE3): key stress factors and parameters for optimisation. Microbial Cell Factories 12, 21. DOI:10.1186/1475-2859-12-21

4. Machado R, Bessa PC, Reis RL, Rodríguez-Cabello JC, Casal M. (2012). Elastin-based nanoparticles for delivery of bone morphogenetic proteins. Methods in Molecular Biology, 906, 353-363. DOI: 10.1007/978-1-61779-953-2_29

3. Bessa PC, Machado R, Nürnberger S, Dopler D, Banerjee A, Cunha AM, Rodríguez-Cabello C, Redl H, van Griensven M, Reis RL, Casal M. (2010). Thermoresponsive self-assembled elastin-based nanoparticles for delivery of BMPs. Journal of Controlled Release, 142(3): 312–318. DOI: 10.1016/j.jconrel.2009.11.003

2. Araújo R, Silva C, Machado R, Casal M, Cunha AM, Rodriguez-Cabello C, Cavaco-Paulo A. (2009). Proteolytic enzyme engineering: a tool for wool. Biomacromolecules, 10(6): 1655-1661. DOI: 10.1002/term.245

1. Machado R, Ribeiro AJ, Padrão J, Silva D, Nobre A, Teixeira JA, Arias FJ, Cunha AM, Rodríguez-Cabello JC, Casal M. (2009). Exploiting the sequence of naturally occuring elastin: construction, production and characterization of a recombinant thermoplastic protein-based polymer. Journal of NanoResearch, 6, 133-145. DOI:10.4028/www.scientific.net/JNanoR.6.133
  1. Development of an ex vivo assay for the characterization of a new elastin-like polymer with antimicrobial properties
    da Costa A, Machado R, Ribeiro A, Gomes A, Rodríguez-Cabello JC, Casal M (2013) Journal of Tissue Engineering and Regenerative Medicine, 7, Suplement s1, Page 11 (TS11). DOI: 10.1002/term.1822
  2. Electrospun recombinant Protein-Based Polymers fibre matrices for skin regeneration
    Machado R, da Costa A, Sencadas V, Gomes A, Lanceros-Mendez S, Rodríguez-Cabello JC, Casal M (2013) Journal of Tissue Engineering and Regenerative Medicine, 7, Suplement s1, Page 12 (TS14). DOI: 10.1002/term.1822
  3. Development of new chimeric proteins for tissue engineering
    Pereira AM, Bernardo TC, da Costa A, Collins T, Machado R, Leonor IB, Casal M, Reis RL (2013) Journal of Tissue Engineering and Regenerative Medicine, 7, Suplement s1, Page 13 (TS16). DOI: 10.1002/term.1822
  4. Optimising a cell factory system for the bioproduction of silk-elastin-like polymers
    Collins T, Barroca M, Branca F, Azevedo-Silva J, da Costa A, Machado R, Casal M (2013) 1st Internacional Conference on Natural Fibres (ICNF2013): Sustainable Materials for Advanced Applications, page 127. Raul Fangueiro (Editor), Guimarães, Portugal. ISBN: 978-989-20-3872-8
  5. Novel nanocellulose reinforced recombinant protein based polymer composites
    Campos AR, Oliveira B, Machado R, Collins T, Azevedo-Silva J, da Costa A, Immonen K, Wikström L, Casal M (2013) 1st Internacional Conference on Natural Fibres (ICNF2013): Sustainable Materials for Advanced Applications, page 333. Raul Fangueiro (Editor), Guimarães, Portugal. ISBN: 978-989-20-3872-8
  6. Synthesis and processing of recombinant silk and elastin copolymers
    Machado R, da Costa A, Sencadas V, Azevedo-Silva J, Collins T, Arias FJ, Lanceros-Mendez S, Rodríguez-Cabello JC, Casal M (2013) 1st Internacional Conference on Natural Fibres (ICNF2013): Sustainable Materials for Advanced Applications, page 153. Raul Fangueiro (Editor), Guimarães, Portugal. ISBN: 978-989-20-3872-8
  7. Tunable morphology and structural properties of recombinant silk-elastinlike biopolymers by electrospinning
    Machado R, García-Arévalo C, Arias FJ, Rodríguez-Cabello JC, Casal M (2011) Biophysical Journal, 100, Issue 3, Supplement 1, Page 369a. DOI: 10.1016/j.bpj.2010.12.2202
  8. Production and purification of recombinant antimicrobial peptides by exploiting the thermoresponsiveness of the elastin-like polymer based on the VPAVG sequence
    da Costa A, Machado R, Matamá T, Rodríguez-Cabello JC, Cavaco-Paulo A, Casal M (2010) Arquivos Portugueses das Ciências Biológicas, Tomo XXXI-XXXII, Fascículo I-IV, p. 151 (P137). Sociedade Portuguesa de Biologia, Margarida Casal, Editor. Braga, Portugal.
  9. Creating new functional biomaterials: construction and production of bone morphogenetic 2-ELP hybrid proteins
    Azevedo Silva J, Machado R, Reis RL, Rodríguez-Cabello JC, Casal M (2010) Arquivos Portugueses das Ciências Biológicas, Tomo XXXI-XXXII, Fascículo I-IV, p. 144 (P130). Sociedade Portuguesa de Biologia. Margarida Casal, Editor. Braga, Portugal.
  10. Engineering of recombinant silk and elastin copolymers
    Machado R, Arias FJ, Rodríguez-Cabello JC, Casal M (2010) Arquivos Portugueses das Ciências Biológicas, Tomo XXXI-XXXII, Fascículo I-IV, p. 138 (OC124). Sociedade Portuguesa de Biologia. Margarida Casal, Editor. Braga, Portugal.
  11. A colecção de estirpes autóctones de Saccharomyces cerevisiae das principais regiões vitivinícolas portuguesas
    Vieira E, Drumonde-Neves J, Machado R, Silva P, Gomes AC, Sousa S, Ramos PT, Alemão F, Lima MT, Araújo I, Duarte FL, Santos MA, Casal M, Schuler D (2010) Livro de actas 1: 71-79. 8º Simpósio de Vitivinicultura do Alentejo, Évora, Portugal.
  12. Construction and bioproduction of a “green” synthetic protein-based polymer exhibiting a smart behaviour
    Machado R, Padrão J, Nobre A, Teixeira JA, Cunha AM, Arias FJ, Rodríguez-Cabello JC, Casal M. (2008) Proceedings of the 10th International Chemical and Biological Engineering Conference - CHEMPOR 2008, pp. 99-100. E.C. Ferreira and M. Mota (Eds.), Braga, Portugal. September 4-6. ISBN: 978-972-97810-4-9 
  1. Elastin-based nanoparticles for delivery of bone morphogenetic proteins. Machado R, Bessa PC, Reis RL, Rodríguez-Cabello JC, Casal M in “Nanoparticles in Biology and Medicine: Methods and Applications”, Methods in Molecular Biology, Vol. 906, pp. 353-363, Mikhail Soloviev (Ed.) published by Humana Press, 2012. ISBN: 978-1-61779-952-5; DOI: 10.1007/978-1-61779-953-2_29
  2. Future Trends for Recombinant Protein-Based Polymers: The Case Study of Development and Application of Silk-Elastin-Like Polymers. Casal M, Cunha AM, Machado R in “Bio-based plastics: Materials and applications”, Wiley Series in Renewable Resources, Stephen Kabasci (Ed.) published by John Wiley & Sons, 2013. ISBN: 978-1-119-99400-8; DOI: 10.1002/9781118676646.ch12
  3. Advanced techniques for characterizing bioinspired materials. Machado R & Sencadas V in "Bioinspired Materials for Medical Applications", Lígia Rodrigues and Manuel Mota (Eds.) published by Woodhead Publishing, 2017. ISBN: 978-0-08-100741-9; DOI: 10.1016/B978-0-08-100741-9.00007-3

Process for the treatment of animal fibres aimed at improving the shrink-resistance thereof
Cavaco-Paulo A, Casal M, Araújo R, Silva C, Rodríguez-Cabello JC, Machado R (2010) Patent publication number WO/2010/001356

 
  • Invited Podium Communications
  1. From Applied Biology to Synthetic Biology: When Biology meets Materials Science
    Raul Machado. XV Jornadas de Biologia Aplicada 19-22 February 2014, Universidade do Minho, Braga, Portugal
  2. Abordagens da biotecnologia para o desenvolvimento de novos (bio)materiais: o exemplo da elastina e da seda
    Raul Machado. 2º Encontro de Biotecnologia Microbiana e Farmacêutica 22 May 2013, Faculdade de Farmácia da Universidade do Porto, Porto, Portugal
  3. How to exploit nanobiotechnology to create bioinspired materials
    Raul Machado. Comemorações dos 20 anos do Mestrado em Genética Molecular 13 February 2013, Universidade do Minho, Braga, Portugal
  4. Silk elastinlike polymers: a new class of synthetic biopolymers
    Raul Machado. DBio Seminars Seminários do Departamento de Biologia 18th May of 2011, Universidade do Minho, Braga, Portugal
  5. Design of recombinant elastin-like polymers and their biotech developments
    Raul Machado. Invited lecture for the Commemoration Day of the School of Sciences 22 February 2010, Braga, Portugal
  • Podium Communications
  1. A new highly antimicrobial bio-inspired protein-based polymer designed for medical devices
    da Costa A, Machado R, Ribeiro A, Collins T, Viruthachalam T, Neves-Petersen MT, Rodriguez-Cabello JC, Gomes A, Casal M. International Conference on Antimicrobial Research (ICAR 2014), 1-3 October 2014, Madrid, Spain
  2. Antibacterial and antifungal activity of a new solvent-cast film based on a functionalized elastin-like polymer
    da Costa A, Machado R, Ribeiro A, Gomes A, Rodriguez-Cabello JC, Casal M. MICROBIOTEC '13, 6-8 December 2013, Aveiro, Portugal
  3. Electrospun recombinant Protein-Based Polymers fibre matrices for skin regeneration
    Machado R, da Costa A, Sencadas V, Gomes A, Lanceros-Mendez S, Rodríguez-Cabello JC, Casal M. TERM STEM 2013 (Nanotechnology as a tool for improving tissue engineering and regenerative medicine), 10-12 October 2013, Porto, Portugal
  4. A synthetic biology approach for the development of recombinant protein-based polymers exhibiting antimicrobial properties
    da Costa A, Machado R, Ribeiro A, Gomes A, Rodríguez-Cabello JC, Casal M. European Symposium on Biopolymers (ESBP 2013), 7-9 October 2013, Lisboa, Portugal
  5. Optimising a cell factory system for the bioproduction of silk-elastin-like polymers
    Collins T, Barroca M, Branca F, Azevedo-Silva J, da Costa A, Machado R, Casal M. 1st International Conference on Natural Fibres (ICNF2013), 9-11 June 2013, Guimarães, Portugal
  6. Synthesis and processing of recombinant silk and elastin copolymers
    Machado R, da Costa A, Sencadas V, Azevedo-Silva J, Collins T, Arias FJ, Lanceros-Mendez S, Rodríguez-Cabello JC, Casal M. 1st International Conference on Natural Fibres (ICNF2013), 9-11 June 2013, Guimarães, Portugal
  7. Solvent-cast films of an elastin-like polymer fused to an antimicrobial peptide, ABP-CMP4, exhibits high antibacterial activity against Pseudomonas aeruginosa
    da Costa A, Machado R, Casal M. MicroBiotec11, 1-3 December 2011, Braga, Portugal
  8. Tunable morphology and structural properties of recombinant silk-elastinlike biopolymers by electrospinning
    Machado R, García-Arévalo C, Arias FJ, Rodríguez-Cabello JC, Casal M. Biophysical Society 55th Annual Meeting, 5-9 March 2011, Baltimore, EUA
  9. Engineering of recombinant silk and elastin copolymers
    Machado R, Arias FJ, Rodríguez-Cabello JC, Casal M. XXXV Jornadas Portuguesas de Genética, 31 May – 2 June 2010, Braga, Portugal
  10. A colecção de estirpes autóctones de Saccharomyces cerevisiae das principais regiões vitivinícolas portuguesas
    Vieira E, Drumonde-Neves J, Machado R, Silva P, Gomes AC, Sousa S, Ramos PT, Alemão F, Lima MT, Araújo I, Duarte FL, Santos MA, Casal M, Schuler D. 8º Simpósio de Vitivinicultura do Alentejo, 5-7 May 2010, Évora, Portugal
  11. Thermoresponsive self-assembled elastin-based nanoparticles for delivery of BMPs
    Machado R, Bessa PC, Nürnberger S, Dopler D, Banerjee A, Cunha AM, Rodríguez-Cabello JC, Redl H, van Griensven M, Reis RL, Casal M. 239th ACS National Meeting, 21-25 March 2010, San Francisco, USA
  12. Recombinant elastin-like polymers: from the design towards application
    Machado R, Araújo R, Silva C, Bessa PC, Cunha AM, Teixeira JA, Cavaco-Paulo A, Arias FJ, Reis RL, Rodríguez-Cabello JC, Casal M. MICROBIOTEC '09, 28-30 November 2009, Vilamoura, Portugal
  13. A New recombinant high molecular weight subtilisin for wool finishing applications
    Araújo R, Silva C, Machado R, Rodríguez-Cabello JC, Casal M, Cavaco-Paulo A. COST Action 868, Biotechnical Functionalisation of Renewable Polymeric Materials, 18-19 September 2008, Varna, Bulgária
  14. Construction and bioproduction of a “green” synthetic protein-based polymer exhibiting a smart behaviour
    Machado R, Padrão J, Nobre A, Teixeira J, Cunha AM, Arias FJ, Rodríguez-Cabello JC, Casal M. 10th International Chemical and Biological Engineering Conference, CHEMPOR 2008, 4-6 September 2008, Braga, Portugal
  15. Construction and production of a new “nature friendly” thermoplastic polymer based on the sequence of elastin (VPAVG)220
    Machado R, Ribeiro AJ, Arias FJ, Cunha AM, Rodríguez-Cabello JC, Casal M. 2nd Technology Open Day Materials at Uminho, 15 June 2007, Universidade do Minho, Guimarães, Portugal
  16. Construction and production of a new thermoplastic polymer based on the sequence of elastin (VPAVG)n
    Machado R, Ribeiro AJ, Arias FJ, Cunha AM, Rodríguez-Cabello JC, Casal M. COST Action 868, Biotechnical Functionalisation of Renewable Polymeric Materials, 16-17 April 2007, Sitges, Espanha
  17. Genetic polymorphisms and expression of CDR1, MDR1 and ERG11 genes in fluconazole resistant and sensitive Candida albicans strains
    Machado R, Sampaio P, Correia A, Pais C. Congresso Nacional de Microbiologia, MICRO’2003 Tomar, Portugal (2003)
  • Posters Communications
  1. Fabrication of SELP/Ag nanocomposite materials with antimicrobial properties by electrospinning and solvent casting
    Silva AM, da Costa A, Chitas R, Collins T, Gomes A, Rodríguez-Cabello JC, Lanceros-Méndez S, Sencadas V, Casal M, Machado R. International Conference on Antimicrobial Research (ICAR 2014), 1-3 October 2014, Madrid, Spain
  2. Antibacterial performance of bovine lactoferrin-fish gelatine electrospun nanocomposites
    Padrão J, Machado R, Casal M, Rodrigues LR, Dourado F, Lanceros-Méndez S, Sencadas V. International Conference on Antimicrobial Research (ICAR 2014), 1-3 October 2014, Madrid, Spain
  3. Electrospun silk-elastin-like fiber mats for tissue engineering applications
    Machado R    , da Costa A, Sencadas V, Gomes A, Lanceros-Mendez S, Rodríguez-Cabello JC, Casal M. MICROBIOTEC ’13, 6-8 December 2013, Aveiro, Portugal
  4. Electrospun silk-elastin-like fiber mats with potential application for tissue engineering
    Machado R    , da Costa A, Sencadas V, Gomes A, Lanceros-Mendez S, Rodríguez-Cabello JC, Casal M. Electrospinning, Principles, Possibilities and Practice 2013, 5-6 December 2013, Institute of Physics, London, UK
  5. Fabrication of recombinant silk-elastin-like fiber mats for tissue engineering applications
    Machado R    , da Costa A, Sencadas V, Gomes A, Lanceros-Mendez S, Rodríguez-Cabello JC, Casal M. Materials Today Virtual Conference: Biomaterials, 19-21 November 2013, online conference. http://www.materials.today.com/virtualconference/materials-today-virtual-conference-biomaterials-2013
  6. Development of functionalized recombinant protein-based polymers exhibiting antimicrobial properties
    da Costa A, Machado R, Ribeiro A, Gomes A, Rodríguez-Cabello JC, Casal M. Materials Today Virtual Conference: Biomaterials, 19-21 November 2013, online conference. http://www.materials.today.com/virtualconference/materials-today-virtual-conference-biomaterials-2013
  7. Development of new chimeric proteins for tissue engineering
    Pereira AM, Bernardo TC, da Costa A, Collins T, Machado R, Leonor IB, Casal M, Reis RL. TERM STEM 2013 (Nanotechnology as a tool for improving tissue engineering and regenerative medicine), 10-12 October 2013, Porto, Portugal
  8. Development of an ex vivo assay for the characterization of a new elastin-like polymer with antimicrobial properties
    da Costa A, Machado R, Ribeiro A, Gomes A, Rodríguez-Cabello JC, Casal M. TERM STEM 2013 (Nanotechnology as a tool for improving tissue engineering and regenerative medicine), 10-12 October 2013, Porto, Portugal
  9. Electrospun silk-elastin-like fibre matrices for tissue engineering applications
    Machado R, da Costa A, Sencadas V, Gomes A, Lanceros-Mendez S, Rodríguez-Cabello JC, Casal M. European Symposium on Biopolymers (ESBP 2013), 7-9 October 2013, Lisboa, Portugal
  10. Novel nanocellulose reinforced recombinant protein based polymer composites
    Campos AR, Oliveira B, Machado R, Collins T, Azevedo-Silva J, da Costa A, Immonen K, Wikström L, Casal M. 1st Internacional Conference on Natural Fibres (ICNF2013), 9-11 June 2013, Guimarães, Portugal
  11. Key stress factors and parameters for production optimisation of silk-elastin-like proteins in E. coli
    Collins T, Azevedo-Silva J, da Costa A, Branca F, Machado R, Casal M. 7th Conference on Recombinant Protein Production (RPP7), 6-8 March 2013, Laupheim, Germany
  12. Tunable morphology and structural properties of recombinant silk-elastin biopolymers by electrospinning
    Machado R, García-Arévalo C, Arias FJ, Rodríguez-Cabello JC, Casal M. MICROBIOTEC '11, 1-3 December 2011, Braga, Portugal
  13. High level expression and facile purification of recombinant silk-elastin-like polymers in auto-induction shake flask cultures
    Machado R, Arias FJ, Rodríguez-Cabello JC, Casal M. MicroBiotec11, 1-3 December 2011, Braga, Portugal
  14. Key stress factors and parameters for batch production optimisation of silk-elastin-like proteins in E. coli
    Collins T, Azevedo-Silva J, da Costa A, Machado R, Casal M. MicroBiotec11, 1-3 December 2011, Braga, Portugal
  15. Designing a cost-effective protein purification protocol using elastin like polymer (VPAVG)220 fusion tag
    Azevedo-Silva J, da Costa A, Machado R, Rodriguez-Cabello J C, Casal M. XXXVI Jornadas Portuguesas de Genética, 30 May – 1 June 2011, Coimbra, Portugal
  16. Use of elastin like polymer (VPAVG)220 fusion tags for recombinant protein production and purification
    Azevedo-Silva J, da Costa A, Machado R, Rodriguez-Cabello J C, Casal M. Congresso Nacional de Bioquímica, 15-17 December 2010, Porto, Portugal
  17. Electrospinning of recombinant silk and elastin copolymers
    Machado R    , García-Arévalo C, Arias FJ, Rodríguez-Cabello JC, Casal M. XVII Congresso Nacional de Bioquímica, 15-17 December 2010, Porto, Portugal
  18. Engineering of recombinant silk and elastin copolymers
    Machado R    , Arias FJ, Rodríguez-Cabello JC, Casal M. 4th International meeting on developments in materials, processes and applications of emerging technologies – MPA 2010 – Nanobiotech Conference, 28-30 July 2010, Braga, Portugal
  19. Production and purification of recombinant antimicrobial peptides by exploiting the thermoresponsiveness of the elastin-like polymer based on the VPAVG sequence
    da Costa A, Machado R, Matamá T, Rodríguez-Cabello JC, Cavaco-Paulo A, Casal M. XXXV Jornadas Portuguesas de Genética, 31 May – 2 June 2010, Braga, Portugal
  20. Creating new functional biomaterials: construction and production of bone morphogenetic protein 2-ELP hybrid protein
    Azevedo-Silva J, Machado R, Reis RL, Rodríguez-Cabello JC, Casal M. XXXV Jornadas Portuguesas de Genética, 31 May – 2 June 2010, Braga, Portugal
  21. Thermoresponsive self-assembled elastin-based nanoparticles for delivery of BMPs
    Machado R    , Bessa PC, Nürnberger S, Dopler D, Banerjee A, Cunha AM, Rodríguez-Cabello JC, Redl H, van Griensven M, Reis RL, Casal M. 5th European Symposium in Biopolymers, ESBP 2009, 18-20 November 2009, Funchal, Portugal
  22. Construction, bioproduction and characterization of protein based polymers: exploiting the sequence of naturally occurring elastin and silk fibroin
    Machado R    , Correia C, Padrão J, Nobre A, Cunha AM, Rodríguez-Cabello JC, Casal M. Congresso Nacional MICRO'07 BIOTEC'07-XXXIII JPG, 30 November – 2 December 2007, Lisboa, Portugal
  23. Response surface optimization of the culture medium for production of elastin-like polymers by Escherichia coli
    Silva D, Padrão J, Machado R, Teixeira J, Casal M, Nobre A. Congresso Nacional MICRO'07 BIOTEC'07-XXXIII JPG, 30 November – 2 December 2007, Lisboa, Portugal
  24. Elastin-like protein production kinetics at different environmental conditions in Escherichia coli
    Padrão J, Silva D, Machado R, Nobre A, Casal M. Congresso Nacional MICRO'07 BIOTEC'07-XXXIII JPG, 30 November – 2 December 2007, Lisboa, Portugal
  25. Physical and chemical optimization of a synthetic medium for Pichia pastoris growth
    Padrão J, Machado R, Nobre A, Casal M. XVth National Congress of Biochemistry, 8-10 December 2006, Aveiro, Portugal
  26. Genetic polymorphisms and expression of CDR1, MDR1 and ERG11 genes in fluconazole resistant and sensitive Candida albicans strains
    Sampaio P, Machado R, Correia A, Pina-Vaz C, Costa-de-Oliveira S, Cachapuz I, Gonçalves Rodrigues A, Pais C. 7th ASM Conferences on Candida and candidiasis 18-22 March 2004, Texas, USA
  27. Genetic polymorphisms and expression of CDR1, MDR1 and ERG11 genes in fluconazole resistant and sensitive Candida albicans strains
    Machado R    , Sampaio P, Correia A, Pais C. Congresso Nacional de Microbiologia, MICRO’2003, 29 November – 1 December 2003, Tomar, Portugal

Post-Docs & Students

  • MSc Students

Andreia Maria Azevedo da Silva. Produção de fibras com propriedades antimicrobianas com recurso à técnica de electrofiação. University of Minho, co-supervision of Prof. Dr. Margarida Casal (CBMA) and Dr. Vitor Sencadas (CFUM). Master in Applied Biochemistry, ongoing.

Rute Malarranha Chitas. A molecular biotechnology approach for the development of Silk-Elastin-Like Polymers functionalized with Leukemia Inhibitory Factor. University of Minho, co-supervision of Prof. Dr. Margarida Casal (CBMA) and Prof. Dr. Andreia Gomes (CBMA). Master in Molecular Genetics, ongoing.
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